Institut for Biologi

Aarhus Universitets segl

Mette Marie Busck

Temporal and spatial microbiome dynamics across natural populations of the social spider Stegodyphus dumicola

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Temporal and spatial microbiome dynamics across natural populations of the social spider Stegodyphus dumicola. / Busck, Mette M.; Lund, Marie B.; Bird, Tharina L. et al.

I: FEMS Microbiology Ecology, Bind 98, Nr. 2, 03.2022.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

APA

CBE

MLA

Vancouver

Author

Bibtex

@article{b2d9f948889e4779a3eef6ee1a892e17,
title = "Temporal and spatial microbiome dynamics across natural populations of the social spider Stegodyphus dumicola",
abstract = "Host-symbiont interactions may form obligatory or facultative associations that are context dependent. Long-term studies on microbiome composition from wild populations should assess the temporal and spatial dynamics of host-microbe associations. We characterized the temporal and spatial variation in the bacterial microbiome composition in six populations of the social spider Stegodyphus dumicola for 2.5 years, using 16S rRNA gene amplicon sequencing of whole spiders. Individuals within a nest exhibit highly similar microbiomes, which remain stable over several generations and are not predictably affected by seasonal variation in temperature or humidity. This stability in nest microbiome is likely due to social transmission, whereas drift-like processes during new nest foundations explain variation in host microbiomes between nests. This is supported by the lack of obligate symbionts (i.e. no symbionts are present in all spider individuals). Quantitative PCR analyses showed that the bacterial load of individual spiders is stable in healthy nests but can increase dramatically in perishing nests. These increases are not driven by specific bacterial taxa but likely caused by loss of host immune control under deteriorating conditions. Spider nests show an annual survival rate of approximately 45%, but nest death is not correlated to microbiome composition, and the bacteria found in S. dumicola are not considered to be high virulence pathogens.",
keywords = "16S amplicon, bacterial load, Kaplan–Meier, nest survival, symbiont, time series",
author = "Busck, {Mette M.} and Lund, {Marie B.} and Bird, {Tharina L.} and Bechsgaard, {Jesper S.} and Trine Bilde and Andreas Schramm",
note = "Publisher Copyright: {\textcopyright} The Author(s) 2022. Published by Oxford University Press on behalf of FEMS.",
year = "2022",
month = mar,
doi = "10.1093/femsec/fiac015",
language = "English",
volume = "98",
journal = "F E M S Microbiology Ecology",
issn = "0168-6496",
publisher = "Oxford University Press",
number = "2",

}

RIS

TY - JOUR

T1 - Temporal and spatial microbiome dynamics across natural populations of the social spider Stegodyphus dumicola

AU - Busck, Mette M.

AU - Lund, Marie B.

AU - Bird, Tharina L.

AU - Bechsgaard, Jesper S.

AU - Bilde, Trine

AU - Schramm, Andreas

N1 - Publisher Copyright: © The Author(s) 2022. Published by Oxford University Press on behalf of FEMS.

PY - 2022/3

Y1 - 2022/3

N2 - Host-symbiont interactions may form obligatory or facultative associations that are context dependent. Long-term studies on microbiome composition from wild populations should assess the temporal and spatial dynamics of host-microbe associations. We characterized the temporal and spatial variation in the bacterial microbiome composition in six populations of the social spider Stegodyphus dumicola for 2.5 years, using 16S rRNA gene amplicon sequencing of whole spiders. Individuals within a nest exhibit highly similar microbiomes, which remain stable over several generations and are not predictably affected by seasonal variation in temperature or humidity. This stability in nest microbiome is likely due to social transmission, whereas drift-like processes during new nest foundations explain variation in host microbiomes between nests. This is supported by the lack of obligate symbionts (i.e. no symbionts are present in all spider individuals). Quantitative PCR analyses showed that the bacterial load of individual spiders is stable in healthy nests but can increase dramatically in perishing nests. These increases are not driven by specific bacterial taxa but likely caused by loss of host immune control under deteriorating conditions. Spider nests show an annual survival rate of approximately 45%, but nest death is not correlated to microbiome composition, and the bacteria found in S. dumicola are not considered to be high virulence pathogens.

AB - Host-symbiont interactions may form obligatory or facultative associations that are context dependent. Long-term studies on microbiome composition from wild populations should assess the temporal and spatial dynamics of host-microbe associations. We characterized the temporal and spatial variation in the bacterial microbiome composition in six populations of the social spider Stegodyphus dumicola for 2.5 years, using 16S rRNA gene amplicon sequencing of whole spiders. Individuals within a nest exhibit highly similar microbiomes, which remain stable over several generations and are not predictably affected by seasonal variation in temperature or humidity. This stability in nest microbiome is likely due to social transmission, whereas drift-like processes during new nest foundations explain variation in host microbiomes between nests. This is supported by the lack of obligate symbionts (i.e. no symbionts are present in all spider individuals). Quantitative PCR analyses showed that the bacterial load of individual spiders is stable in healthy nests but can increase dramatically in perishing nests. These increases are not driven by specific bacterial taxa but likely caused by loss of host immune control under deteriorating conditions. Spider nests show an annual survival rate of approximately 45%, but nest death is not correlated to microbiome composition, and the bacteria found in S. dumicola are not considered to be high virulence pathogens.

KW - 16S amplicon

KW - bacterial load

KW - Kaplan–Meier

KW - nest survival

KW - symbiont

KW - time series

UR - http://www.scopus.com/inward/record.url?scp=85126152512&partnerID=8YFLogxK

U2 - 10.1093/femsec/fiac015

DO - 10.1093/femsec/fiac015

M3 - Journal article

C2 - 35147190

AN - SCOPUS:85126152512

VL - 98

JO - F E M S Microbiology Ecology

JF - F E M S Microbiology Ecology

SN - 0168-6496

IS - 2

ER -