Martin Hansen

Isolation and characterisation of novel phages infecting Lactobacillus plantarum and proposal of a new genus, “Silenusvirus”

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

DOI

  • Ifigeneia Kyrkou, Rigshospitalet
  • ,
  • Alexander Byth Carstens, Københavns Universitet
  • ,
  • Lea Ellegaard-Jensen
  • Witold Kot, Københavns Universitet
  • ,
  • Athanasios Zervas
  • Amaru Miranda Djurhuus, Københavns Universitet
  • ,
  • Horst Neve, Max Rubner Institut
  • ,
  • Charles M.A.P. Franz, Max Rubner Institut
  • ,
  • Martin Hansen
  • Lars Hestbjerg Hansen, Københavns Universitet

Bacteria of Lactobacillus sp. are very useful to humans. However, the biology and genomic diversity of their (bacterio)phage enemies remains understudied. Knowledge on Lactobacillus phage diversity should broaden to develop efficient phage control strategies. To this end, organic waste samples were screened for phages against two wine-related Lactobacillus plantarum strains. Isolates were shotgun sequenced and compared against the phage database and each other by phylogenetics and comparative genomics. The new isolates had only three distant relatives from the database, but displayed a high overall degree of genomic similarity amongst them. The latter allowed for the use of one isolate as a representative to conduct transmission electron microscopy and structural protein sequencing, and to study phage adsorption and growth kinetics. The microscopy and proteomics tests confirmed the observed diversity of the new isolates and supported their classification to the family Siphoviridae and the proposal of the new phage genus “Silenusvirus”.

OriginalsprogEngelsk
Artikelnummer8763
TidsskriftScientific Reports
Vol/bind10
Nummer1
Antal sider10
ISSN2045-2322
DOI
StatusUdgivet - 2020

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