Martin Hansen

H295R cells as a model for steroidogenic disruption: a broader perspective using simultaneous chemical analysis of 7 key steroid hormones.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

H295R cells as a model for steroidogenic disruption: a broader perspective using simultaneous chemical analysis of 7 key steroid hormones. / Nielsen, Frederik Knud; Hansen, Cecilie Hurup; Fey, Jennifer Anna; Hansen, Martin; Jacobsen, Naja Wessel; Halling-Sørensen, Bent; Björklund, Erland; Styrishave, Bjarne.

I: Toxicology in vitro : an international journal published in association with BIBRA, Bind 26, Nr. 2, 01.03.2012, s. 343-350.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Nielsen, FK, Hansen, CH, Fey, JA, Hansen, M, Jacobsen, NW, Halling-Sørensen, B, Björklund, E & Styrishave, B 2012, 'H295R cells as a model for steroidogenic disruption: a broader perspective using simultaneous chemical analysis of 7 key steroid hormones.', Toxicology in vitro : an international journal published in association with BIBRA, bind 26, nr. 2, s. 343-350. <http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=22198065&retmode=ref&cmd=prlinks>

APA

Nielsen, F. K., Hansen, C. H., Fey, J. A., Hansen, M., Jacobsen, N. W., Halling-Sørensen, B., Björklund, E., & Styrishave, B. (2012). H295R cells as a model for steroidogenic disruption: a broader perspective using simultaneous chemical analysis of 7 key steroid hormones. Toxicology in vitro : an international journal published in association with BIBRA, 26(2), 343-350. http://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=22198065&retmode=ref&cmd=prlinks

CBE

Nielsen FK, Hansen CH, Fey JA, Hansen M, Jacobsen NW, Halling-Sørensen B, Björklund E, Styrishave B. 2012. H295R cells as a model for steroidogenic disruption: a broader perspective using simultaneous chemical analysis of 7 key steroid hormones. Toxicology in vitro : an international journal published in association with BIBRA. 26(2):343-350.

MLA

Nielsen, Frederik Knud o.a.. "H295R cells as a model for steroidogenic disruption: a broader perspective using simultaneous chemical analysis of 7 key steroid hormones.". Toxicology in vitro : an international journal published in association with BIBRA. 2012, 26(2). 343-350.

Vancouver

Nielsen FK, Hansen CH, Fey JA, Hansen M, Jacobsen NW, Halling-Sørensen B o.a. H295R cells as a model for steroidogenic disruption: a broader perspective using simultaneous chemical analysis of 7 key steroid hormones. Toxicology in vitro : an international journal published in association with BIBRA. 2012 mar 1;26(2):343-350.

Author

Nielsen, Frederik Knud ; Hansen, Cecilie Hurup ; Fey, Jennifer Anna ; Hansen, Martin ; Jacobsen, Naja Wessel ; Halling-Sørensen, Bent ; Björklund, Erland ; Styrishave, Bjarne. / H295R cells as a model for steroidogenic disruption: a broader perspective using simultaneous chemical analysis of 7 key steroid hormones. I: Toxicology in vitro : an international journal published in association with BIBRA. 2012 ; Bind 26, Nr. 2. s. 343-350.

Bibtex

@article{8ab6c1c49a3147868656540fcf5491c7,
title = "H295R cells as a model for steroidogenic disruption: a broader perspective using simultaneous chemical analysis of 7 key steroid hormones.",
abstract = "The effects of three model endocrine disruptors, prochloraz, ketoconazole and genistein on steroidogenesis were tested in the adrenocortical H295R cell line to demonstrate that a broader mechanistic understanding can be achieved in one assay by applying chemical analysis to the H295R assay. Seven key steroid hormones (pregnenolone, progesterone, dehydroepiandrosterone, androstenedione, testosterone, estrone and 17β-estradiol) were analyzed using a novel and thoroughly validated GC-MS/MS method. In addition to the simultaneous quantification of 7 steroid hormones, the present method also negates the potential problems of cross-reactivity that can be encountered in some immunoassays. Although all 3 test compounds decrease the concentrations of the main sex steroids, the chemicals exerted different effects upstream in the pathway. Exposure to prochloraz resulted in increased hormone levels upstream of steroid 17 alpha-hydroxylase/17,20 lyase (P450c17) and decreases downstream. Ketoconazole inhibited the entire pathway, while exposure to genistein resulted in increased hormone levels upstream of 3-β-hydroxysteroid dehydrogenase (3β-HSD) and decreases downstream. The results demonstrate that chemical analysis combined with the H295R cell assay is an useful tool for studying the mechanisms by which endocrine disruptors interfere with the steroidogenic pathway.",
author = "Nielsen, {Frederik Knud} and Hansen, {Cecilie Hurup} and Fey, {Jennifer Anna} and Martin Hansen and Jacobsen, {Naja Wessel} and Bent Halling-S{\o}rensen and Erland Bj{\"o}rklund and Bjarne Styrishave",
year = "2012",
month = mar,
day = "1",
language = "English",
volume = "26",
pages = "343--350",
journal = "Toxicology in vitro : an international journal published in association with BIBRA",
number = "2",

}

RIS

TY - JOUR

T1 - H295R cells as a model for steroidogenic disruption: a broader perspective using simultaneous chemical analysis of 7 key steroid hormones.

AU - Nielsen, Frederik Knud

AU - Hansen, Cecilie Hurup

AU - Fey, Jennifer Anna

AU - Hansen, Martin

AU - Jacobsen, Naja Wessel

AU - Halling-Sørensen, Bent

AU - Björklund, Erland

AU - Styrishave, Bjarne

PY - 2012/3/1

Y1 - 2012/3/1

N2 - The effects of three model endocrine disruptors, prochloraz, ketoconazole and genistein on steroidogenesis were tested in the adrenocortical H295R cell line to demonstrate that a broader mechanistic understanding can be achieved in one assay by applying chemical analysis to the H295R assay. Seven key steroid hormones (pregnenolone, progesterone, dehydroepiandrosterone, androstenedione, testosterone, estrone and 17β-estradiol) were analyzed using a novel and thoroughly validated GC-MS/MS method. In addition to the simultaneous quantification of 7 steroid hormones, the present method also negates the potential problems of cross-reactivity that can be encountered in some immunoassays. Although all 3 test compounds decrease the concentrations of the main sex steroids, the chemicals exerted different effects upstream in the pathway. Exposure to prochloraz resulted in increased hormone levels upstream of steroid 17 alpha-hydroxylase/17,20 lyase (P450c17) and decreases downstream. Ketoconazole inhibited the entire pathway, while exposure to genistein resulted in increased hormone levels upstream of 3-β-hydroxysteroid dehydrogenase (3β-HSD) and decreases downstream. The results demonstrate that chemical analysis combined with the H295R cell assay is an useful tool for studying the mechanisms by which endocrine disruptors interfere with the steroidogenic pathway.

AB - The effects of three model endocrine disruptors, prochloraz, ketoconazole and genistein on steroidogenesis were tested in the adrenocortical H295R cell line to demonstrate that a broader mechanistic understanding can be achieved in one assay by applying chemical analysis to the H295R assay. Seven key steroid hormones (pregnenolone, progesterone, dehydroepiandrosterone, androstenedione, testosterone, estrone and 17β-estradiol) were analyzed using a novel and thoroughly validated GC-MS/MS method. In addition to the simultaneous quantification of 7 steroid hormones, the present method also negates the potential problems of cross-reactivity that can be encountered in some immunoassays. Although all 3 test compounds decrease the concentrations of the main sex steroids, the chemicals exerted different effects upstream in the pathway. Exposure to prochloraz resulted in increased hormone levels upstream of steroid 17 alpha-hydroxylase/17,20 lyase (P450c17) and decreases downstream. Ketoconazole inhibited the entire pathway, while exposure to genistein resulted in increased hormone levels upstream of 3-β-hydroxysteroid dehydrogenase (3β-HSD) and decreases downstream. The results demonstrate that chemical analysis combined with the H295R cell assay is an useful tool for studying the mechanisms by which endocrine disruptors interfere with the steroidogenic pathway.

M3 - Journal article

VL - 26

SP - 343

EP - 350

JO - Toxicology in vitro : an international journal published in association with BIBRA

JF - Toxicology in vitro : an international journal published in association with BIBRA

IS - 2

ER -