Martin Hansen

Expanding the diversity of Myoviridae phages infecting Lactobacillus plantarum - a novel lineage of Lactobacillus phages comprising five new members

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Expanding the diversity of Myoviridae phages infecting Lactobacillus plantarum - a novel lineage of Lactobacillus phages comprising five new members. / Kyrkou, Ifigeneia; Carstens, Alexander Byth; Ellegaard-Jensen, Lea; Kot, Witold; Zervas, Athanasios; Djurhuus, Amaru Miranda; Neve, Horst; Hansen, Martin; Hansen, Lars Hestbjerg.

I: Viruses, Bind 11, Nr. 7, 611, 2019.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Kyrkou, Ifigeneia ; Carstens, Alexander Byth ; Ellegaard-Jensen, Lea ; Kot, Witold ; Zervas, Athanasios ; Djurhuus, Amaru Miranda ; Neve, Horst ; Hansen, Martin ; Hansen, Lars Hestbjerg. / Expanding the diversity of Myoviridae phages infecting Lactobacillus plantarum - a novel lineage of Lactobacillus phages comprising five new members. I: Viruses. 2019 ; Bind 11, Nr. 7.

Bibtex

@article{d4fe9156f52b49dab08f06a72b8b666a,
title = "Expanding the diversity of Myoviridae phages infecting Lactobacillus plantarum - a novel lineage of Lactobacillus phages comprising five new members",
abstract = "Lactobacillus plantarum is a bacterium with promising applications to the food industry and agriculture and probiotic properties. So far, bacteriophages of this bacterium have been moderately addressed. We examined the morphology and diversity of five new L. plantarum phages via transmission electron microscopy, whole genome shotgun sequencing and in silico protein predictions. Moreover, we looked into their phylogeny and their potential genomic similarities to other complete phage genome records through extensive nucleotide and protein comparisons. The five phages share a high degree of similarity among them and belong to the family Myoviridae. They have a long genome of 137.973-141.344 bp, a G/C content of 36,3-36,6% that is quite distinct from their host{\textquoteright}s, and, surprisingly, seven to 15 tRNAs. Only an average 35/174 of their predicted genes were assigned a function. Overall, the comparative analyses unraveled considerable genetic diversity for our five L. plantarum phages. Hence, the new genus “Semelevirus” was proposed, which comprises exclusively the five phages. This novel lineage of Lactobacillus phages provides further insight into the genetic heterogeneity of phages infecting Lactobacillus sp. Our results on Lactobacillus phages have a potential value for the development of anti-phage strategies as well as for the manipulation of L. plantarum fermentations.",
author = "Ifigeneia Kyrkou and Carstens, {Alexander Byth} and Lea Ellegaard-Jensen and Witold Kot and Athanasios Zervas and Djurhuus, {Amaru Miranda} and Horst Neve and Martin Hansen and Hansen, {Lars Hestbjerg}",
year = "2019",
doi = "10.3390/v11070611",
language = "English",
volume = "11",
journal = "Viruses",
issn = "1999-4915",
publisher = "M D P I AG",
number = "7",

}

RIS

TY - JOUR

T1 - Expanding the diversity of Myoviridae phages infecting Lactobacillus plantarum - a novel lineage of Lactobacillus phages comprising five new members

AU - Kyrkou, Ifigeneia

AU - Carstens, Alexander Byth

AU - Ellegaard-Jensen, Lea

AU - Kot, Witold

AU - Zervas, Athanasios

AU - Djurhuus, Amaru Miranda

AU - Neve, Horst

AU - Hansen, Martin

AU - Hansen, Lars Hestbjerg

PY - 2019

Y1 - 2019

N2 - Lactobacillus plantarum is a bacterium with promising applications to the food industry and agriculture and probiotic properties. So far, bacteriophages of this bacterium have been moderately addressed. We examined the morphology and diversity of five new L. plantarum phages via transmission electron microscopy, whole genome shotgun sequencing and in silico protein predictions. Moreover, we looked into their phylogeny and their potential genomic similarities to other complete phage genome records through extensive nucleotide and protein comparisons. The five phages share a high degree of similarity among them and belong to the family Myoviridae. They have a long genome of 137.973-141.344 bp, a G/C content of 36,3-36,6% that is quite distinct from their host’s, and, surprisingly, seven to 15 tRNAs. Only an average 35/174 of their predicted genes were assigned a function. Overall, the comparative analyses unraveled considerable genetic diversity for our five L. plantarum phages. Hence, the new genus “Semelevirus” was proposed, which comprises exclusively the five phages. This novel lineage of Lactobacillus phages provides further insight into the genetic heterogeneity of phages infecting Lactobacillus sp. Our results on Lactobacillus phages have a potential value for the development of anti-phage strategies as well as for the manipulation of L. plantarum fermentations.

AB - Lactobacillus plantarum is a bacterium with promising applications to the food industry and agriculture and probiotic properties. So far, bacteriophages of this bacterium have been moderately addressed. We examined the morphology and diversity of five new L. plantarum phages via transmission electron microscopy, whole genome shotgun sequencing and in silico protein predictions. Moreover, we looked into their phylogeny and their potential genomic similarities to other complete phage genome records through extensive nucleotide and protein comparisons. The five phages share a high degree of similarity among them and belong to the family Myoviridae. They have a long genome of 137.973-141.344 bp, a G/C content of 36,3-36,6% that is quite distinct from their host’s, and, surprisingly, seven to 15 tRNAs. Only an average 35/174 of their predicted genes were assigned a function. Overall, the comparative analyses unraveled considerable genetic diversity for our five L. plantarum phages. Hence, the new genus “Semelevirus” was proposed, which comprises exclusively the five phages. This novel lineage of Lactobacillus phages provides further insight into the genetic heterogeneity of phages infecting Lactobacillus sp. Our results on Lactobacillus phages have a potential value for the development of anti-phage strategies as well as for the manipulation of L. plantarum fermentations.

U2 - 10.3390/v11070611

DO - 10.3390/v11070611

M3 - Journal article

C2 - 31277436

VL - 11

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 7

M1 - 611

ER -