Marco Eijken

Two-day-treatment of Activin-A leads to transient change in SV-HFO osteoblast gene expression and reduction in matrix mineralization

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review


  • Marta Baroncelli, University Medical Center Rotterdam
  • ,
  • Ksenija Drabek, University Medical Center Rotterdam
  • ,
  • Marco Eijken
  • Bram C J van der Eerden, University Medical Center Rotterdam
  • ,
  • Jeroen van de Peppel, University Medical Center Rotterdam
  • ,
  • Johannes P T M van Leeuwen, University Medical Center Rotterdam

Activins regulate bone formation by controlling osteoclasts and osteoblasts. We investigated Activin-A mechanism of action on human osteoblast mineralization, RNA and microRNA (miRNA) expression profile. A single 2-day treatment of Activin-A at Day 5 of osteoblast differentiation significantly reduced matrix mineralization. Activin A-treated osteoblasts responded with transient change in gene expression, in a 2-wave-fashion. The 38 genes differentially regulated during the first wave (within 8 hr after Activin A start) were involved in transcription regulation. In the second wave (1-2 days after Activin A start), 65 genes were differentially regulated and related to extracellular matrix. Differentially expressed genes in both waves were associated to transforming growth factor beta signaling. We identified which microRNAs modulating osteoblast differentiation were regulated by Activin-A. In summary, 2-day treatment with Activin-A in premineralization period of osteoblast cultures influenced miRNAs, gene transcription, and reduced matrix mineralization. Modulation of Activin A signaling might be useful to control bone quality for therapeutic purposes.

TidsskriftJournal of Cellular Physiology
Sider (fra-til)4865-4877
Antal sider13
StatusUdgivet - maj 2020
Eksternt udgivetJa

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