Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review
Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Effects of dexamethasone-loaded PLGA microspheres on human fetal osteoblasts
AU - Dawes, G J S
AU - Fratila-Apachitei, L E
AU - Necula, B S
AU - Apachitei, I
AU - van Leeuwen, J P T M
AU - Duszczyk, J
AU - Eijken, M
PY - 2012/11
Y1 - 2012/11
N2 - Integration of a drug delivery function into implantable medical devices enables local release of specific bioactives to control cells-surface interactions. One alternative to achieve this biofunctionality for bone implants is to incorporate particulate drug delivery systems (DDSs) into the rough or porous implant surfaces. The scope of this study was to assess the effects of a model DDS consisting of poly(D,L-lactide-co-glycolide) (PLGA) microspheres loaded with an anti-inflammatory drug, dexamethasone (DXM), on the response of Simian Virus-immortalized Human Fetal Osteoblast (SV-HFO) cells. The microspheres were prepared by the oil-in-water emulsion/solvent evaporation method, whereas cells response was investigated by Alamar Blue test for viability, alkaline phosphatase (ALP) activity for differentiation, and Alizarin Red staining for matrix mineralization. Cell viability was not affected by the presence of increased concentrations of polymeric microspheres in the culture media. Furthermore, in the cultures with DXM-loaded microspheres, ALP activity was expressed at levels similar with those obtained under osteogenic conditions, indicating that DXM released from the microsphere-stimulated cell differentiation. Matrix mineralization occurred preferentially around the DXM-loaded microspheres confirming that the released DXM could act as osteogenic supplement for the cells. These in vitro findings suggest that a particulate PLGA-DXM DDS may actually provide dual, anti-inflammatory and osteogenic functions when incorporated on the surface of bone implants.
AB - Integration of a drug delivery function into implantable medical devices enables local release of specific bioactives to control cells-surface interactions. One alternative to achieve this biofunctionality for bone implants is to incorporate particulate drug delivery systems (DDSs) into the rough or porous implant surfaces. The scope of this study was to assess the effects of a model DDS consisting of poly(D,L-lactide-co-glycolide) (PLGA) microspheres loaded with an anti-inflammatory drug, dexamethasone (DXM), on the response of Simian Virus-immortalized Human Fetal Osteoblast (SV-HFO) cells. The microspheres were prepared by the oil-in-water emulsion/solvent evaporation method, whereas cells response was investigated by Alamar Blue test for viability, alkaline phosphatase (ALP) activity for differentiation, and Alizarin Red staining for matrix mineralization. Cell viability was not affected by the presence of increased concentrations of polymeric microspheres in the culture media. Furthermore, in the cultures with DXM-loaded microspheres, ALP activity was expressed at levels similar with those obtained under osteogenic conditions, indicating that DXM released from the microsphere-stimulated cell differentiation. Matrix mineralization occurred preferentially around the DXM-loaded microspheres confirming that the released DXM could act as osteogenic supplement for the cells. These in vitro findings suggest that a particulate PLGA-DXM DDS may actually provide dual, anti-inflammatory and osteogenic functions when incorporated on the surface of bone implants.
KW - Alkaline Phosphatase/metabolism
KW - Anti-Inflammatory Agents/administration & dosage
KW - Cell Differentiation/drug effects
KW - Cell Line
KW - Cell Survival/drug effects
KW - Coated Materials, Biocompatible/chemistry
KW - Dexamethasone/administration & dosage
KW - Drug Delivery Systems
KW - Humans
KW - Lactic Acid/chemistry
KW - Microspheres
KW - Osteoblasts/cytology
KW - Polyglycolic Acid/chemistry
KW - Polylactic Acid-Polyglycolic Acid Copolymer
KW - Prostheses and Implants
U2 - 10.1177/0885328211412634
DO - 10.1177/0885328211412634
M3 - Journal article
C2 - 21862514
VL - 27
SP - 477
EP - 483
JO - Journal of Biomedical Materials Research. Part B: Applied Biomaterials
JF - Journal of Biomedical Materials Research. Part B: Applied Biomaterials
SN - 1552-4973
IS - 4
ER -