Institut for Biomedicin

Marco Capogna

Properties of spontaneous miniature GABAA receptor mediated synaptic currents in area CA3 of rat hippocampal slice cultures

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

  • S M Thompson, Brain Research Institute, University of Zurich, CH-8029 Zurich, Switzerland.
  • ,
  • J C Poncer
  • ,
  • M Capogna
  • B H Gähwiler

Miniature, gamma-aminobutyric acid A receptor mediated inhibitory postsynaptic currents (mIPSCs) were recorded from CA3 pyramidal cells in hippocampal slice cultures using whole-cell techniques in the presence of tetrodotoxin. The kinetics and amplitudes of the mIPSCs were analyzed with the aim of determining whether subclasses of events arising from distinct populations of presynaptic interneurons could be distinguished. Histograms of mIPSC amplitude, rise time constant, and decay time constant were all positively skewed, but discrete subsets of events could not be distinguished. The positive skew did not appear to result from electrotonic filtering of distal synaptic currents because there was no correlation among mIPSC amplitudes and the kinetic parameters. Analysis of the intervals between mIPSCs indicated that each event occurred independently. The analysis of spontaneous mIPSCs does not provide evidence of the innervation of pyramidal cells by heterogeneous interneurons.

OriginalsprogEngelsk
TidsskriftCanadian Journal of Physiology and Pharmacology
Vol/bind75
Nummer5
Sider (fra-til)495-9
Antal sider5
ISSN0008-4212
StatusUdgivet - maj 1997

Se relationer på Aarhus Universitet Citationsformater

ID: 141167654