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Manfred Schmid

Rrp6p controls mRNA poly(a) tail length and its decoration with poly(a) binding proteins

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  • Manfred Schmid
  • Mathias Bach Poulsen, Danmark
  • Pawel Olszewski, Danmark
  • Vincent Pelecano, Genome Biology Unit, EMBL Heidelberg, Tyskland
  • Cyril Saguez, Danmark
  • Ishaan Gupta, Genome Biology Unit, EMBL Heidelberg, Tyskland
  • Lars M Steinmetz, Genome Biology Unit, EMBL Heidelberg, Tyskland
  • Claire Moore, Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, USA
  • Torben Heick Jensen
Poly(A) (pA) tail binding proteins (PABPs) control mRNA polyadenylation, stability, and translation. In a purified system, S. cerevisiae PABPs, Pab1p and Nab2p, are individually sufficient to provide normal pA tail length. However, it is unknown how this occurs in more complex environments. Here we find that the nuclear exosome subunit Rrp6p counteracts the in vitro and in vivo extension of mature pA tails by the noncanonical pA polymerase Trf4p. Moreover, PABP loading onto nascent pA tails is controlled by Rrp6p; while Pab1p is the major PABP, Nab2p only associates in the absence of Rrp6p. This is because Rrp6p can interact with Nab2p and displace it from pA tails, potentially leading to RNA turnover, as evidenced for certain pre-mRNAs. We suggest that a nuclear mRNP surveillance step involves targeting of Rrp6p by Nab2p-bound pA-tailed RNPs and that pre-mRNA abundance is regulated at this level
OriginalsprogEngelsk
TidsskriftMolecular Cell
Vol/bind47
Nummer2
Sider (fra-til)267-280
Antal sider13
ISSN1097-2765
StatusUdgivet - 27 jul. 2012

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