Lise Wogensen

Functional and morphological effects of interleukin-1 beta on the perfused rat pancreas

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Functional and morphological effects of interleukin-1 beta on the perfused rat pancreas. / Wogensen, L D; Kolb-Bachofen, V; Christensen, P; Dinarello, C A; Mandrup-Poulsen, T; Martin, S; Nerup, J.

I: Diabetologia, Bind 33, Nr. 1, 01.1990, s. 15-23.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Wogensen, LD, Kolb-Bachofen, V, Christensen, P, Dinarello, CA, Mandrup-Poulsen, T, Martin, S & Nerup, J 1990, 'Functional and morphological effects of interleukin-1 beta on the perfused rat pancreas', Diabetologia, bind 33, nr. 1, s. 15-23.

APA

Wogensen, L. D., Kolb-Bachofen, V., Christensen, P., Dinarello, C. A., Mandrup-Poulsen, T., Martin, S., & Nerup, J. (1990). Functional and morphological effects of interleukin-1 beta on the perfused rat pancreas. Diabetologia, 33(1), 15-23.

CBE

Wogensen LD, Kolb-Bachofen V, Christensen P, Dinarello CA, Mandrup-Poulsen T, Martin S, Nerup J. 1990. Functional and morphological effects of interleukin-1 beta on the perfused rat pancreas. Diabetologia. 33(1):15-23.

MLA

Vancouver

Wogensen LD, Kolb-Bachofen V, Christensen P, Dinarello CA, Mandrup-Poulsen T, Martin S o.a. Functional and morphological effects of interleukin-1 beta on the perfused rat pancreas. Diabetologia. 1990 jan;33(1):15-23.

Author

Wogensen, L D ; Kolb-Bachofen, V ; Christensen, P ; Dinarello, C A ; Mandrup-Poulsen, T ; Martin, S ; Nerup, J. / Functional and morphological effects of interleukin-1 beta on the perfused rat pancreas. I: Diabetologia. 1990 ; Bind 33, Nr. 1. s. 15-23.

Bibtex

@article{fae72609616841ad8487bdf234d083da,
title = "Functional and morphological effects of interleukin-1 beta on the perfused rat pancreas",
abstract = "We recently reported a potentiating effect of recombinant human interleukin-1 beta on glucose-stimulated insulin release from the isolated perfused pancreas. With the aim of determining whether the stimulatory effect of recombinant interleukin-1 beta on the B cell in the intact gland was modulated by varying the concentration, time of exposure to recombinant interleukin-1 beta or B-cell activity, and to elucidate a possible mechanism of action, we measured in the perfused rat pancreas the release of insulin, glucagon and/or prostaglandin E2 according to the following three different protocols: (1) perfusion with 20 ng/ml of recombinant interleukin-1 beta for 92 min at 5 and 20 mmol/l D-glucose (2) perfusion with varying concentrations of recombinant interleukin-1 beta ranging from 0.1 x 10(-3) ng/ml to 100 ng/ml at 5 and 20 mmol/l D-glucose (3) perfusion with 20 ng/ml of recombinant interleukin-1 beta at 5, 11 or 20 mmol/l D-glucose. Furthermore, in a separate set of experiments we examined the influence of the cytokine on the morphology of the endocrine pancreas. Interleukin-1 beta stimulated insulin secretion at 11 and 20 mmol/l D-glucose and potentiated first as well as second phase insulin release in a dose-dependent fashion, with decreasing effect at higher concentrations. Glucagon secretion was also stimulated by recombinant interleukin-1 beta, irrespective of increasing glucose (5, 11, 20 mmol/l) and insulin concentrations. The potentiating effect of recombinant interleukin-1 beta on insulin secretion was evident even after discontinued perfusion with the cytokine, suggesting a priming effect on B-cell function.(ABSTRACT TRUNCATED AT 250 WORDS)",
keywords = "Animals, Dinoprostone, Glucagon, Glucose, In Vitro Techniques, Insulin, Interleukin-1, Islets of Langerhans, Kinetics, Male, Microscopy, Electron, Perfusion, Rats, Rats, Inbred Strains, Recombinant Proteins",
author = "Wogensen, {L D} and V Kolb-Bachofen and P Christensen and Dinarello, {C A} and T Mandrup-Poulsen and S Martin and J Nerup",
year = "1990",
month = jan,
language = "English",
volume = "33",
pages = "15--23",
journal = "Diabetologia",
issn = "0012-186X",
publisher = "Springer",
number = "1",

}

RIS

TY - JOUR

T1 - Functional and morphological effects of interleukin-1 beta on the perfused rat pancreas

AU - Wogensen, L D

AU - Kolb-Bachofen, V

AU - Christensen, P

AU - Dinarello, C A

AU - Mandrup-Poulsen, T

AU - Martin, S

AU - Nerup, J

PY - 1990/1

Y1 - 1990/1

N2 - We recently reported a potentiating effect of recombinant human interleukin-1 beta on glucose-stimulated insulin release from the isolated perfused pancreas. With the aim of determining whether the stimulatory effect of recombinant interleukin-1 beta on the B cell in the intact gland was modulated by varying the concentration, time of exposure to recombinant interleukin-1 beta or B-cell activity, and to elucidate a possible mechanism of action, we measured in the perfused rat pancreas the release of insulin, glucagon and/or prostaglandin E2 according to the following three different protocols: (1) perfusion with 20 ng/ml of recombinant interleukin-1 beta for 92 min at 5 and 20 mmol/l D-glucose (2) perfusion with varying concentrations of recombinant interleukin-1 beta ranging from 0.1 x 10(-3) ng/ml to 100 ng/ml at 5 and 20 mmol/l D-glucose (3) perfusion with 20 ng/ml of recombinant interleukin-1 beta at 5, 11 or 20 mmol/l D-glucose. Furthermore, in a separate set of experiments we examined the influence of the cytokine on the morphology of the endocrine pancreas. Interleukin-1 beta stimulated insulin secretion at 11 and 20 mmol/l D-glucose and potentiated first as well as second phase insulin release in a dose-dependent fashion, with decreasing effect at higher concentrations. Glucagon secretion was also stimulated by recombinant interleukin-1 beta, irrespective of increasing glucose (5, 11, 20 mmol/l) and insulin concentrations. The potentiating effect of recombinant interleukin-1 beta on insulin secretion was evident even after discontinued perfusion with the cytokine, suggesting a priming effect on B-cell function.(ABSTRACT TRUNCATED AT 250 WORDS)

AB - We recently reported a potentiating effect of recombinant human interleukin-1 beta on glucose-stimulated insulin release from the isolated perfused pancreas. With the aim of determining whether the stimulatory effect of recombinant interleukin-1 beta on the B cell in the intact gland was modulated by varying the concentration, time of exposure to recombinant interleukin-1 beta or B-cell activity, and to elucidate a possible mechanism of action, we measured in the perfused rat pancreas the release of insulin, glucagon and/or prostaglandin E2 according to the following three different protocols: (1) perfusion with 20 ng/ml of recombinant interleukin-1 beta for 92 min at 5 and 20 mmol/l D-glucose (2) perfusion with varying concentrations of recombinant interleukin-1 beta ranging from 0.1 x 10(-3) ng/ml to 100 ng/ml at 5 and 20 mmol/l D-glucose (3) perfusion with 20 ng/ml of recombinant interleukin-1 beta at 5, 11 or 20 mmol/l D-glucose. Furthermore, in a separate set of experiments we examined the influence of the cytokine on the morphology of the endocrine pancreas. Interleukin-1 beta stimulated insulin secretion at 11 and 20 mmol/l D-glucose and potentiated first as well as second phase insulin release in a dose-dependent fashion, with decreasing effect at higher concentrations. Glucagon secretion was also stimulated by recombinant interleukin-1 beta, irrespective of increasing glucose (5, 11, 20 mmol/l) and insulin concentrations. The potentiating effect of recombinant interleukin-1 beta on insulin secretion was evident even after discontinued perfusion with the cytokine, suggesting a priming effect on B-cell function.(ABSTRACT TRUNCATED AT 250 WORDS)

KW - Animals

KW - Dinoprostone

KW - Glucagon

KW - Glucose

KW - In Vitro Techniques

KW - Insulin

KW - Interleukin-1

KW - Islets of Langerhans

KW - Kinetics

KW - Male

KW - Microscopy, Electron

KW - Perfusion

KW - Rats

KW - Rats, Inbred Strains

KW - Recombinant Proteins

M3 - Journal article

C2 - 2406177

VL - 33

SP - 15

EP - 23

JO - Diabetologia

JF - Diabetologia

SN - 0012-186X

IS - 1

ER -