Aarhus University Seal / Aarhus Universitets segl

Lars Ditlev Mørck Ottosen

Konstitueret institutleder


Assistant Professor, Aarhus University

Ph.D. Microbial Ecology, Bioscience, Aarhus University.

Exam Oecon, Business Economy, Aarhus University (Institute of Economics)

MSc. Biology, Aarhus University


Associate Professor, Head of Biological and Chemical Engineering Section, Department of Engineering, Aarhus University

Head of Section Environmental Biotechnology, Danish Technological Institute

Consultant R&D, Danish Technological Institute

Assistant Professor, Aarhus University

2004- 2007/3
Clinical Embryologist and Project Scientist, Aarhus University Hospital

Manager Business development, Unisense A/S

Ph.D Scholar, Biological Institute, Microbial Ecology, Aarhus University

Teaching assistant, Aarhus University

Technical assistant (plant genetics and breeding) Nordic Seed

Academic research

Post Doctoral Research at AU, Department of microbiology. Applied research on biological airfilters and animal slurry chemistry and microbiology.

2,5 years as part time (approx. 50%) clinical researcher at Aarhus University Hospital, Fertility clinic and Clinical Institute. Research area: preimplantation embryo physiology including method and technology development, clinical epidemiology and related research.

Ph.D. research (4+4 model). Microbial Ecology, nitrogen transformation in marine and aquatic systems.

Industrial research

Industrial research and development at Danish Technological Institute. Research, development and documentation within environmental biotechnology and technology including water treatment technologies, anaerobic digestion and substrate pre-treatment, biorefining, various aspects of environmental technology.

Technology development for Unisense A/S (techniques for measuring embryo oxygen consumption). Collaborative research projects (clinical epidemiology) with pharmaceutical industry

Industrial research at Unisense A/S. Applied research on microsensor development and applications, Unisense A/S contribution in a large EU projects (ICON), research on bovine embryos at former Danmarks Jordbrugsforskning.


Appointed Head of Section, Environmental Technology at Life Science division DTI. The job responsibility includes line management, section economy, budgeting and recruiting academic staff.

Selected by board of directors at Danish Technological Institute for “Talentprogram” leadership and business development training. (ECTS points ?)


4 academic positions filled 2 ongoing recruitments. Course in job analysis prior to recruiting, course in interview technique related to recruiting.

Teaching and supervision

2014 -
Teaching Waste Water Treatment in the 5 ECTS course "Applied Microbiology"
Teaching the 5 ETCS course "Industrial Fermentation"
Teaching in the 5 ECTS course "Air Quality Engineering"

External supervisor at Aarhus School of Engineering (supervision of a total of 6 diploma engineering students, all graduated with note 12)

Completed “Kursus i Universitetspædagogik for adjunkter”

Geomicrobiology (10 ECTS) 2008 and Biotechnique (5 ECTS) 2009, both with excellent student rating.
Co-supervision of master students, Ph.D. students.

Clinical embryology at the Organon Fertility School (2006)

Lectures, laboratory courses, and excursions in General Microbiology, Microbial Ecology, Microbial Physiology and Aquatic Microbiology at the University of Aarhus, Denmark (During Ph.D scholarship 1996-2000)

Related Professional Experience, Activities

Reviewer for MEPS (1998-2009) and Human Reproduction (since 2005-2010)

Scientific expert, Danish Environmental Technology Verification (DAN-ETV) 2009.

Co-author of Medical Technology Verification (MTV) reports for the Danish National
Board of Health (2005-2007).

Responsible for patenting and IPR at Unisense A/S (2000-2004) and completed Patent Course (Patentkursus) (2001) AU og Østjysk Innovation. Extended experience with the patenting process.

Board member Unisense A/S (2002) and Alpha Biobank Aps (2005)

Co-founder owner and board member at start up biotech company Alpha Biobank Aps (2005). The company was successfully taken over by another biotech company in 2006.

Task force member for certification (DS ISO) of clinical procedures at the Fertility Clinic, Aarhus University hospital. (2003-2004)

Awarded for best article series of the year by Danish technical periodical “Water and Soil” (1998)

Research interest and expertise

Microbial ecology (analysis of microbial activity, metabolic pathways, inhibition). Microbial nitrogen, carbon and sulfur cycling in natural system and industrial and agricultural systems and installations. Microbial production of green house gasses.
Microphysiology and microscale chemical gradients.

Industrial biotechnology:
Biological and chemical waste water treatment. Biogas process and technology (mechanics, chemistry and microbiology of the process).

Medical biotechnology:
Physiology of pre implantation embryos and clinical embryology and epidemiology. Experimental design of trials.

Selected courses

Adjunkt kursus i universitetspædagogik

Patentkursus AU og Østjysk Innovation

Recent large grants obtained

Obtained > 1 million DKK, 2010-2012, personally and under my supervision:

BIOMAN (Research for the benefit of SME EU FP7) > 5 million DKK.

Technology for hospital waste water treatment (MUDP) 4,6 million DKK

BIOCONVAL (Grønt Udvikling og Demonstrations Program GUDP) 6,9 million DKK

Lavenergivask, rent og sikkert (Fornyelsesfonden) 7,3 million DKK

Selective hydrolysis for waste water sludge (VTU) 1 mio DKK

Examples of funding sources where I have extended experience:
-Det Strategiske Forskningsråd (National Strategic Reseach Council)
-VTU fonden
-EcoInnovation (Miljøstyrelsen)
-Fornyelsesfonden grønne løsninger
-Videnkuponer (store og små)

-FP 7 EU programmes (eg KBBE)



Ottosen LDM, Susanne Juhler, Lise Bonne Guldberg, Anders Feilberg, Niels Peter Revsbech, Lars Peter Nielsen. Regulation of Ammonia Oxidation in Biotrickling Airfilters with high Ammonium load. 2011, Chemical Engineering 167: 198–205

Ottosen LDM, Poulsen HV, Aagreen D, Finster K, Nielsen LPN, Revsbech NP. 2008 Observations on Microbial activity in acidified pig slurry. Biosystems Engineering (2009) doi:10.101

Susanne Juhler, Niels Peter Revsbech, Andreas Schramm, Martina Herrmann, Lars DM Ottosen, Lars Peter Nielsen (2009) Distribution and activity of microbial processes in ammonia-loaded air filter biofilm. Applied Environmetal Microbiology 75(11) 3705-3713

Ottosen LDM. Hindkjær, Ingerslev J (2007) Murine pre-embryo oxygen consumption and developmental competence. J Assist Reprod Genet. 24(8):359-65 PMID: 17636441

Ottosen LDM, Hindkjær J, Husth M, Petersen DE, Kirk J, Ingerslev HJ (2006) Observations on intrauterine oxygen tension measured by fibre-optic microsensors. Reproductive BioMedicine Online 13 (3) 380-85

Ottosen LDM, Damgaard LR, Ramsing NB, Gundersen JK (2003) Device and method for noninvasive measurement of the individual metabolic rate of a substantially spherical metabolizing particle. Published Patent application WO2004056265

Ottosen LDM, Risgaard-Petersen N, Nielsen LP, Dalsgaard T (2001) Denitrification in exposed intertidal sediments. Mar Ecol Prog Ser 209: 35-42.

Ottosen LDM, Risgaard-Petersen N, Nielsen LP (1999) Direct and indirect measurements of nitrification and denitrification in the rhizosphere of aquatic macrophytes. Aquat Microb Ecol 19: 81-91

Ottosen LDM. Hindkjær J, Ingerslev J, Kesmodel U (2007) Pregnancy prediction models and simple inclusion criteria for allocating IVF/ICSI patients to eSET, do we have the right information? J Assist Reprod Genet. 24(1):29-36 PMID: 17165151

Ottosen LDM, Hindkjær J, Ingerslev J (2007) Light exposure of the ovum and preimplantation embryoduring ART procedures. J Assist Reprod Genet. 24(2-3):99-103. PMID: 17216346

Højgaard A, Ottosen LD, Kesmodel U, Ingerslev HJ (2007) Patient attitudes towards twin pregnancies and single embryo transfer - A questionnaire study. Hum Reprod. 22(10):2673-8 PMID: 17766921

Ebbesen SMS, R Zachariae, MY Mehlsen, D Thomsen, A Højgaard, L Ottosen, T Petersen, HJ Ingerslev (2009). Stressful life events are associated with a poor IVF-outcome: Results from a prospective quantitative study. Human Reproduction 2009. DOI 10.1093/humrep/dep 186

Anne Sørensen, Michael Pedersen, Anna Tietze, Lars DM Ottosen, Liv Duus, Niels Uldbjerg (2008) BOLD MRI: a non invasive measurement of changes in tissue oxygenation in fetal sheep. Ultrasound in Obstetrics and Gynecology. 2009

Markfoged R, Nyord T, Revsbech NP, Ottosen LDM, Nielsen LPN 2010. Transient N2O accumulation and emission caused by O2 depletion in soil after liquid manure injection. European Journal of Soil Science. DOI: 10.1111/j.1365-2389.2011.01369.

Risgaard-Petersen N, Ottosen LDM (2000) Nitrogen cycling in two temperate Zostera marina bed: seasonal variation. Mar Ecol Prog Ser 198: 93-107

Jetten M, Schmid M, Schmidt I, Ottosen LDM, Wagner M, Kuenen G et al. (2002) Improved Nitrogen removal by application of a new nitrogen-cycle bacteria. Reviews in Environmental Science and Biotechnology 1: 51-63.

Brotas V, Risgaard-Petersen N, Serôdio J, Ottosen LDM, Dalsgaard T, Ribeiro L (2003). In situ measurement of photosynthetic activity and respiration of intertidal benthic microalgal communities undergoing vertical migration Ophelia 57: 13-26

Nielsen TG, Ottosen LDM, Hansen BW (2007) Structure and function of the pelagic ecosystem in Young Sound, NE Greenland. In: Rysgaard S & Glud R.N (eds), Carbon cycling in Arctic Marine Ecosystems : Case study Young Sound. Meddr. Grønland, Bioscience 58:88-107.


Markfoged R, Nyord T, Revsbech NP, Ottosen LDM, Nielsen LPN (in Prep) Temporal N2O events and spatial N2O distribution in soils with surface applied and injected liquid pig manure as the consequence of the anoxia evolution.

Nielsen M, Ottosen LDM, Damgaard LR and Revsbech NP. (In Prep) A H2S insensitive H2 microsensor.


Ingerslev HJ, Poulsen PB, Kesmodel U, Højgaard A, Pindborg A, Henriksen TB, Seeberg J, Ottosen LDM. Should one or two embryos be transferred in IVF? A health technology assessment Danish Health technology assessment 2005; 7(2) Copenhagen: National board of health, Danish Centre for Evaluation and Health Technology Assessment, 2005.

Ottosen, L.D.M., Fossing, H. & Rysgaard, S. (2000): Mineralization in the Water Column and Sea Ice. In: Caning, K. & Rasch, M. (eds.): Zackenberg Ecological Research Operations, ZERO, 5th Annual Report 1999. Danish Polar Center. pp. 67-68.

Thamdrup, B., Fossing, H., Hansen, J.W., Ottosen, L.D.M. & Rysgaard, S. (2000): Sedimentation and Mineralization in the Sediment. In: Caning, K. & Rasch, M. (eds.): Zackenberg Ecological Research Operations, ZERO, 5th Annual Report 1999. Danish Polar Center. pp. 68-69.

Dalsgaard TD (ed.), Nielsen LP, Brotas V, Ottosen LDM et al. (2000): Protocol handbook for
NICE- Nitrogen Cycling In Estuaries: a project under the EU research programme: Marine Science and Technology (MAST III). National Environmental Research Institute, Silkeborg, Denmark. 62pp.

Ottosen LDM (1997) Pricing Nature (In Danish, “Sæt pris på miljøet”). Vand og Jord 1(4): 21-23

Ottosen LDM (1997) Valuation of Environmental goods (In Danish, “Værdisætning af miljøgoder”. Vand og Jord 2(4): 85-87)

Ottosen LDM (1998) Transplantation of eelgrass (In Danish, “Udplantning af ålegræs, kan det betale sig?”) Vand og Jord 1(5): 36-39


Ottosen LDM, Nielsen LP, Juhler S et al. Function of biological airfilters for pig production.


C. Kragelund, K. Litty1, Ravi Chhetri, Lars Ottosen, C. Sund, H-U. Rasmussen, M. Christensson and H.R. Andersen (2013) Energy-efficient treatment of hospital wastewater. 13th Nordic Wastewater Conference. October 8–10, 2013 in Malmö, Sweden.

C. Kragelund1*, K. Litty1, Ravi Chhetri2, Lars Ottosen1, C. Sund3, H-U. Rasmussen4, M. Christensson5 and H.R. Andersen2 Energy-efficient treatment of hospital wastewater. 2013 Congress of The Danish Microbiological Society, November 18th, 2013 in Copenhagen, Denmark

H. Uellendahl, S.I. Njoku, C. Kragelund, L. Ottosen, B. Ruiz (2013). Boosting the economy of manure based biogas plants. Proceedings of the International Anaerobic Digestion Symposium at BiogasWorld 2013, April 23-25, Berlin. Oral presentation

H. Uellendahl, L. Ottosen, B. Ruiz, B. Malmgren-Hansen, L. Bailón Allegue, J. Hinge, S.I. Njoku, and C. Kragelund (2013) BIOMAN. `Economically efficient biogas production from manure fibres and straw´.EU BC&E- 21st European Biomass Conference and Exhibition. 3-7 June, Copenhagen, Denmark. Poster presentation

C. Kragelund, M.V.W. Kofoed, L. Bjerrum and L.D.M. Ottosen (2012). Configuration consequences in waste water treatment plants on the nitrifying community. Poster presentation at Danish Society of Microbiology (DMS 2012), Copenhagen, November 2012.

Lars DM Ottosen, Anne-Belinda Bjerre, Jørgen Hinge and Caroline Kragelund (2012). BIOMAN
`Economically efficient biogas production from manure fibres and straw´. Short oral presentation at Danish Society of Microbiology (DMS 2012), Copenhagen, November 2012.

Kragelund, C., Kofoed, M.V.W., Bjerrum, L., Ottosen, L.D.M., Saunders, A.M. (2012). Comparison of conventional wastewater treatment plant and a MBR plant by nitrification rates, qPCR and pyrosequencing. Oral presentation at the Danish Water Research Platform (DWRP12), Copenhagen, Denmark, January 2012.

Sorensen; M. Pedersen; A. Tietze; L. Ottosen; L. Duus; N. Uldbjerg (2008) BOLD MRI: a reliable non-invasive method for measuring changes in fetal tissue oxygenation
Top 5 Abstract . 18th World Congress on Ultrasound in Obstetrics and Gynecolegy Chicago, USA. August 24 -28, 2008

Lopes A, Ottosen LDM, Greve T, Callesen H (2003) Oxygen indicators of culture conditions and embryo viability. Conference abstract and Poster presentation for International Embryo Transfer Society meeting New Zeeland 2004.

Lopes A, Ottosen LDM, Greve T, Callesen H (2002) Microsensor measurements of oxygen conditions around the in vitro developing cattle embryo: preliminary observations. Conference abstract and Poster presentation for International Embryo Transfer Society meeting New Zeeland 2003.

Poulsen PB, Ingerslev HJ, Kesmodel U, Højgaard , A, Pinborg A, Henriksen TB, Ottosen LD, Seeberg (2006) Cost effectiveness of single embryo transfer (SET) versus double embryo transfer (DET) strategies in in-vitro fertilisation. Poster presentation at the ISPOR 9th Annual European Congress, Copenhagen, Denmark, October 28-31, 2006.

Ottosen, L., Fossing, H. & Rysgaard, S.: Pelagic N-Mineralization under Arctic Ice Cover.
ASLO 2000 Aquatic Sciences Meeting, Research Across Boundaries, Copenhagen, Denmark, June 5-9, 2000.

Ottosen, L et al. 2007. Oxygen consumption and developmental potential of murine pre implantation embryos. ESHRE conference July 6-9 2007, Lyon, France

Ottosen LDM. Biofilm formation, characterization, bad guys and good guys. Præsentation på Danske Lægers Sårselskabs årsmøde, Skejby maj 2008.

Ottosen, LDM. Beyond Kyoto: Microbiology and how to manage GHG emission from live stock waste. Oral presentation. March 2009

Juhler S, Ottosen L, Nielsen LP, Schramm A, Revsbech NP 2008. Controlling factors of nitrite oxidation in ammonia removing biological air filters. Poster ISME Australia 2008.

Nielsen LP, Juhler S, Ottosen LDM 2008. Function of biofilters removing ammonia from pig
house ventilation air CIGR - International Conference of Agricultural Engineering

Nødgaard A, Pedersen M, Ottosen LDM, Uldbjerg N, Duus L. A non invasive measurement of changes in tissue oxygenation in fetal sheep.Top 5 Abstract (oral presentation). 18th World Congress on Ultrasound in Obstetrics and Gynecolegy Chicago, USA. August 24 -28, 2008

Nødgaard A, Pedersen M, Ottosen LDM, Uldbjerg N, Duus L. a non invasive measurement of changes in tissue oxygenation in fetal sheep. Abstract (poster) 8.th World Congress of Perinatal Medicine, Florence, Italy. September 9 - 13, 2007

Ottosen, LDM et al. International Society Microbial Ecology (ISME 2010) Ammonia removal limited by inhibition of metabolism and growth.