Institut for Biomedicin

Lars Bolund

Systematic in vitro and in vivo characterization of Leukemia-inhibiting factor- and Fibroblast growth factor-derived porcine induced pluripotent stem cells

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Systematic in vitro and in vivo characterization of Leukemia-inhibiting factor- and Fibroblast growth factor-derived porcine induced pluripotent stem cells. / Secher, Jan O; Ceylan, Ahmet; Mazzoni, Gianluca; Mashayekhi, Kaveh; Li, Tong; Muenthaisong, Suchitra; Nielsen, Troels T; Li, Dong; Li, Shengting; Petkov, Stoyan; Cirera, Susanna; Luo, Yonglun; Thombs, Lori; Kadarmideen, Haja N; Dinnyes, Andras; Bolund, Lars; Roelen, Bernard A J; Schmidt, Mette; Callesen, Henrik; Hyttel, Poul; Freude, Kristine K.

I: Molecular Reproduction and Development, Bind 84, Nr. 3, 2017, s. 229-245.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Secher, JO, Ceylan, A, Mazzoni, G, Mashayekhi, K, Li, T, Muenthaisong, S, Nielsen, TT, Li, D, Li, S, Petkov, S, Cirera, S, Luo, Y, Thombs, L, Kadarmideen, HN, Dinnyes, A, Bolund, L, Roelen, BAJ, Schmidt, M, Callesen, H, Hyttel, P & Freude, KK 2017, 'Systematic in vitro and in vivo characterization of Leukemia-inhibiting factor- and Fibroblast growth factor-derived porcine induced pluripotent stem cells', Molecular Reproduction and Development, bind 84, nr. 3, s. 229-245. https://doi.org/10.1002/mrd.22771

APA

Secher, J. O., Ceylan, A., Mazzoni, G., Mashayekhi, K., Li, T., Muenthaisong, S., Nielsen, T. T., Li, D., Li, S., Petkov, S., Cirera, S., Luo, Y., Thombs, L., Kadarmideen, H. N., Dinnyes, A., Bolund, L., Roelen, B. A. J., Schmidt, M., Callesen, H., ... Freude, K. K. (2017). Systematic in vitro and in vivo characterization of Leukemia-inhibiting factor- and Fibroblast growth factor-derived porcine induced pluripotent stem cells. Molecular Reproduction and Development, 84(3), 229-245. https://doi.org/10.1002/mrd.22771

CBE

Secher JO, Ceylan A, Mazzoni G, Mashayekhi K, Li T, Muenthaisong S, Nielsen TT, Li D, Li S, Petkov S, Cirera S, Luo Y, Thombs L, Kadarmideen HN, Dinnyes A, Bolund L, Roelen BAJ, Schmidt M, Callesen H, Hyttel P, Freude KK. 2017. Systematic in vitro and in vivo characterization of Leukemia-inhibiting factor- and Fibroblast growth factor-derived porcine induced pluripotent stem cells. Molecular Reproduction and Development. 84(3):229-245. https://doi.org/10.1002/mrd.22771

MLA

Vancouver

Secher JO, Ceylan A, Mazzoni G, Mashayekhi K, Li T, Muenthaisong S o.a. Systematic in vitro and in vivo characterization of Leukemia-inhibiting factor- and Fibroblast growth factor-derived porcine induced pluripotent stem cells. Molecular Reproduction and Development. 2017;84(3):229-245. https://doi.org/10.1002/mrd.22771

Author

Secher, Jan O ; Ceylan, Ahmet ; Mazzoni, Gianluca ; Mashayekhi, Kaveh ; Li, Tong ; Muenthaisong, Suchitra ; Nielsen, Troels T ; Li, Dong ; Li, Shengting ; Petkov, Stoyan ; Cirera, Susanna ; Luo, Yonglun ; Thombs, Lori ; Kadarmideen, Haja N ; Dinnyes, Andras ; Bolund, Lars ; Roelen, Bernard A J ; Schmidt, Mette ; Callesen, Henrik ; Hyttel, Poul ; Freude, Kristine K. / Systematic in vitro and in vivo characterization of Leukemia-inhibiting factor- and Fibroblast growth factor-derived porcine induced pluripotent stem cells. I: Molecular Reproduction and Development. 2017 ; Bind 84, Nr. 3. s. 229-245.

Bibtex

@article{50dd9cac7920464c9eba4ceb48995fc2,
title = "Systematic in vitro and in vivo characterization of Leukemia-inhibiting factor- and Fibroblast growth factor-derived porcine induced pluripotent stem cells",
abstract = "Derivation and stable maintenance of porcine induced pluripotent stem cells (piPSCs) is challenging. We herein systematically analyzed two piPSC lines, derived by lentiviral transduction and cultured under either leukemia inhibitory factor (LIF) or fibroblast growth factor (FGF) conditions, to shed more light on the underlying biological mechanisms of porcine pluripotency. LIF-derived piPSCs were more successful than their FGF-derived counterparts in the generation of in vitro chimeras and in teratoma formation. When LIF piPSCs chimeras were transferred into surrogate sows and allowed to develop, only their prescence within the embryonic membranes could be detected. Whole transcriptome analysis of the piPSCs and porcine neonatal fibroblasts showed that they clustered together, but apart from the two pluripotent cell populations of early porcine embryos, indicating incomplete reprogramming. Indeed, bioinformatic analysis of the pluripotency-related gene network of the LIF- versus FGF-derived piPSCs revealed that ZFP42 (REX1) expression was absent in both piPSC-like cells, whereas it was expressed in the porcine inner cell mass at Day 7/8. A second striking difference was the expression of ATOH1 in piPSC-like cells, which was absent in the inner cell mass. Moreover, our gene expression analyses plus correlation analyses of known pluripotency genes identified unique relationships between pluripotency genes in the inner cell mass, which are to some extend, in the piPSC-like cells. This deficiency in downstream gene activation and divergent gene expression may be underlie the inability to derive germ line-transmitting piPSCs, and provides unique insight into which genes are necessary to achieve fully reprogrammed piPSCs. This article is protected by copyright. All rights reserved.",
author = "Secher, {Jan O} and Ahmet Ceylan and Gianluca Mazzoni and Kaveh Mashayekhi and Tong Li and Suchitra Muenthaisong and Nielsen, {Troels T} and Dong Li and Shengting Li and Stoyan Petkov and Susanna Cirera and Yonglun Luo and Lori Thombs and Kadarmideen, {Haja N} and Andras Dinnyes and Lars Bolund and Roelen, {Bernard A J} and Mette Schmidt and Henrik Callesen and Poul Hyttel and Freude, {Kristine K}",
note = "This article is protected by copyright. All rights reserved.",
year = "2017",
doi = "10.1002/mrd.22771",
language = "English",
volume = "84",
pages = "229--245",
journal = "Molecular Reproduction and Development",
issn = "1040-452X",
publisher = "JohnWiley & Sons, Inc.",
number = "3",

}

RIS

TY - JOUR

T1 - Systematic in vitro and in vivo characterization of Leukemia-inhibiting factor- and Fibroblast growth factor-derived porcine induced pluripotent stem cells

AU - Secher, Jan O

AU - Ceylan, Ahmet

AU - Mazzoni, Gianluca

AU - Mashayekhi, Kaveh

AU - Li, Tong

AU - Muenthaisong, Suchitra

AU - Nielsen, Troels T

AU - Li, Dong

AU - Li, Shengting

AU - Petkov, Stoyan

AU - Cirera, Susanna

AU - Luo, Yonglun

AU - Thombs, Lori

AU - Kadarmideen, Haja N

AU - Dinnyes, Andras

AU - Bolund, Lars

AU - Roelen, Bernard A J

AU - Schmidt, Mette

AU - Callesen, Henrik

AU - Hyttel, Poul

AU - Freude, Kristine K

N1 - This article is protected by copyright. All rights reserved.

PY - 2017

Y1 - 2017

N2 - Derivation and stable maintenance of porcine induced pluripotent stem cells (piPSCs) is challenging. We herein systematically analyzed two piPSC lines, derived by lentiviral transduction and cultured under either leukemia inhibitory factor (LIF) or fibroblast growth factor (FGF) conditions, to shed more light on the underlying biological mechanisms of porcine pluripotency. LIF-derived piPSCs were more successful than their FGF-derived counterparts in the generation of in vitro chimeras and in teratoma formation. When LIF piPSCs chimeras were transferred into surrogate sows and allowed to develop, only their prescence within the embryonic membranes could be detected. Whole transcriptome analysis of the piPSCs and porcine neonatal fibroblasts showed that they clustered together, but apart from the two pluripotent cell populations of early porcine embryos, indicating incomplete reprogramming. Indeed, bioinformatic analysis of the pluripotency-related gene network of the LIF- versus FGF-derived piPSCs revealed that ZFP42 (REX1) expression was absent in both piPSC-like cells, whereas it was expressed in the porcine inner cell mass at Day 7/8. A second striking difference was the expression of ATOH1 in piPSC-like cells, which was absent in the inner cell mass. Moreover, our gene expression analyses plus correlation analyses of known pluripotency genes identified unique relationships between pluripotency genes in the inner cell mass, which are to some extend, in the piPSC-like cells. This deficiency in downstream gene activation and divergent gene expression may be underlie the inability to derive germ line-transmitting piPSCs, and provides unique insight into which genes are necessary to achieve fully reprogrammed piPSCs. This article is protected by copyright. All rights reserved.

AB - Derivation and stable maintenance of porcine induced pluripotent stem cells (piPSCs) is challenging. We herein systematically analyzed two piPSC lines, derived by lentiviral transduction and cultured under either leukemia inhibitory factor (LIF) or fibroblast growth factor (FGF) conditions, to shed more light on the underlying biological mechanisms of porcine pluripotency. LIF-derived piPSCs were more successful than their FGF-derived counterparts in the generation of in vitro chimeras and in teratoma formation. When LIF piPSCs chimeras were transferred into surrogate sows and allowed to develop, only their prescence within the embryonic membranes could be detected. Whole transcriptome analysis of the piPSCs and porcine neonatal fibroblasts showed that they clustered together, but apart from the two pluripotent cell populations of early porcine embryos, indicating incomplete reprogramming. Indeed, bioinformatic analysis of the pluripotency-related gene network of the LIF- versus FGF-derived piPSCs revealed that ZFP42 (REX1) expression was absent in both piPSC-like cells, whereas it was expressed in the porcine inner cell mass at Day 7/8. A second striking difference was the expression of ATOH1 in piPSC-like cells, which was absent in the inner cell mass. Moreover, our gene expression analyses plus correlation analyses of known pluripotency genes identified unique relationships between pluripotency genes in the inner cell mass, which are to some extend, in the piPSC-like cells. This deficiency in downstream gene activation and divergent gene expression may be underlie the inability to derive germ line-transmitting piPSCs, and provides unique insight into which genes are necessary to achieve fully reprogrammed piPSCs. This article is protected by copyright. All rights reserved.

U2 - 10.1002/mrd.22771

DO - 10.1002/mrd.22771

M3 - Journal article

C2 - 28044390

VL - 84

SP - 229

EP - 245

JO - Molecular Reproduction and Development

JF - Molecular Reproduction and Development

SN - 1040-452X

IS - 3

ER -