Institut for Biomedicin

Lars Bolund

Single-Cell RNA Sequencing Maps Endothelial Metabolic Plasticity in Pathological Angiogenesis

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Single-Cell RNA Sequencing Maps Endothelial Metabolic Plasticity in Pathological Angiogenesis. / Rohlenova, Katerina; Goveia, Jermaine; García-Caballero, Melissa; Subramanian, Abhishek; Kalucka, Joanna; Treps, Lucas; Falkenberg, Kim D; de Rooij, Laura P M H; Zheng, Yingfeng; Lin, Lin; Sokol, Liliana; Teuwen, Laure-Anne; Geldhof, Vincent; Taverna, Federico; Pircher, Andreas; Conradi, Lena-Christin; Khan, Shawez; Stegen, Steve; Panovska, Dena; De Smet, Frederik; Staal, Frank J T; Mclaughlin, Rene J; Vinckier, Stefan; Van Bergen, Tine; Ectors, Nadine; De Haes, Patrik; Wang, Jian; Bolund, Lars; Schoonjans, Luc; Karakach, Tobias K; Yang, Huanming; Carmeliet, Geert; Liu, Yizhi; Thienpont, Bernard; Dewerchin, Mieke; Eelen, Guy; Li, Xuri; Luo, Yonglun; Carmeliet, Peter.

I: Cell Metabolism, Bind 31, Nr. 4, 04.2020, s. 862-877.e14.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Rohlenova, K, Goveia, J, García-Caballero, M, Subramanian, A, Kalucka, J, Treps, L, Falkenberg, KD, de Rooij, LPMH, Zheng, Y, Lin, L, Sokol, L, Teuwen, L-A, Geldhof, V, Taverna, F, Pircher, A, Conradi, L-C, Khan, S, Stegen, S, Panovska, D, De Smet, F, Staal, FJT, Mclaughlin, RJ, Vinckier, S, Van Bergen, T, Ectors, N, De Haes, P, Wang, J, Bolund, L, Schoonjans, L, Karakach, TK, Yang, H, Carmeliet, G, Liu, Y, Thienpont, B, Dewerchin, M, Eelen, G, Li, X, Luo, Y & Carmeliet, P 2020, 'Single-Cell RNA Sequencing Maps Endothelial Metabolic Plasticity in Pathological Angiogenesis', Cell Metabolism, bind 31, nr. 4, s. 862-877.e14. https://doi.org/10.1016/j.cmet.2020.03.009

APA

Rohlenova, K., Goveia, J., García-Caballero, M., Subramanian, A., Kalucka, J., Treps, L., Falkenberg, K. D., de Rooij, L. P. M. H., Zheng, Y., Lin, L., Sokol, L., Teuwen, L-A., Geldhof, V., Taverna, F., Pircher, A., Conradi, L-C., Khan, S., Stegen, S., Panovska, D., ... Carmeliet, P. (2020). Single-Cell RNA Sequencing Maps Endothelial Metabolic Plasticity in Pathological Angiogenesis. Cell Metabolism, 31(4), 862-877.e14. https://doi.org/10.1016/j.cmet.2020.03.009

CBE

Rohlenova K, Goveia J, García-Caballero M, Subramanian A, Kalucka J, Treps L, Falkenberg KD, de Rooij LPMH, Zheng Y, Lin L, Sokol L, Teuwen L-A, Geldhof V, Taverna F, Pircher A, Conradi L-C, Khan S, Stegen S, Panovska D, De Smet F, Staal FJT, Mclaughlin RJ, Vinckier S, Van Bergen T, Ectors N, De Haes P, Wang J, Bolund L, Schoonjans L, Karakach TK, Yang H, Carmeliet G, Liu Y, Thienpont B, Dewerchin M, Eelen G, Li X, Luo Y, Carmeliet P. 2020. Single-Cell RNA Sequencing Maps Endothelial Metabolic Plasticity in Pathological Angiogenesis. Cell Metabolism. 31(4):862-877.e14. https://doi.org/10.1016/j.cmet.2020.03.009

MLA

Vancouver

Rohlenova K, Goveia J, García-Caballero M, Subramanian A, Kalucka J, Treps L o.a. Single-Cell RNA Sequencing Maps Endothelial Metabolic Plasticity in Pathological Angiogenesis. Cell Metabolism. 2020 apr;31(4):862-877.e14. https://doi.org/10.1016/j.cmet.2020.03.009

Author

Rohlenova, Katerina ; Goveia, Jermaine ; García-Caballero, Melissa ; Subramanian, Abhishek ; Kalucka, Joanna ; Treps, Lucas ; Falkenberg, Kim D ; de Rooij, Laura P M H ; Zheng, Yingfeng ; Lin, Lin ; Sokol, Liliana ; Teuwen, Laure-Anne ; Geldhof, Vincent ; Taverna, Federico ; Pircher, Andreas ; Conradi, Lena-Christin ; Khan, Shawez ; Stegen, Steve ; Panovska, Dena ; De Smet, Frederik ; Staal, Frank J T ; Mclaughlin, Rene J ; Vinckier, Stefan ; Van Bergen, Tine ; Ectors, Nadine ; De Haes, Patrik ; Wang, Jian ; Bolund, Lars ; Schoonjans, Luc ; Karakach, Tobias K ; Yang, Huanming ; Carmeliet, Geert ; Liu, Yizhi ; Thienpont, Bernard ; Dewerchin, Mieke ; Eelen, Guy ; Li, Xuri ; Luo, Yonglun ; Carmeliet, Peter. / Single-Cell RNA Sequencing Maps Endothelial Metabolic Plasticity in Pathological Angiogenesis. I: Cell Metabolism. 2020 ; Bind 31, Nr. 4. s. 862-877.e14.

Bibtex

@article{97efb1dded1340afb2193469074cf9b9,
title = "Single-Cell RNA Sequencing Maps Endothelial Metabolic Plasticity in Pathological Angiogenesis",
abstract = "Endothelial cell (EC) metabolism is an emerging target for anti-angiogenic therapy in tumor angiogenesis and choroidal neovascularization (CNV), but little is known about individual EC metabolic transcriptomes. By single-cell RNA sequencing 28,337 murine choroidal ECs (CECs) and sprouting CNV-ECs, we constructed a taxonomy to characterize their heterogeneity. Comparison with murine lung tumor ECs (TECs) revealed congruent marker gene expression by distinct EC phenotypes across tissues and diseases, suggesting similar angiogenic mechanisms. Trajectory inference predicted that differentiation of venous to angiogenic ECs was accompanied by metabolic transcriptome plasticity. ECs displayed metabolic transcriptome heterogeneity during cell-cycle progression and in quiescence. Hypothesizing that conserved genes are important, we used an integrated analysis, based on congruent transcriptome analysis, CEC-tailored genome-scale metabolic modeling, and gene expression meta-analysis in cross-species datasets, followed by in vitro and in vivo validation, to identify SQLE and ALDH18A1 as previously unknown metabolic angiogenic targets.",
author = "Katerina Rohlenova and Jermaine Goveia and Melissa Garc{\'i}a-Caballero and Abhishek Subramanian and Joanna Kalucka and Lucas Treps and Falkenberg, {Kim D} and {de Rooij}, {Laura P M H} and Yingfeng Zheng and Lin Lin and Liliana Sokol and Laure-Anne Teuwen and Vincent Geldhof and Federico Taverna and Andreas Pircher and Lena-Christin Conradi and Shawez Khan and Steve Stegen and Dena Panovska and {De Smet}, Frederik and Staal, {Frank J T} and Mclaughlin, {Rene J} and Stefan Vinckier and {Van Bergen}, Tine and Nadine Ectors and {De Haes}, Patrik and Jian Wang and Lars Bolund and Luc Schoonjans and Karakach, {Tobias K} and Huanming Yang and Geert Carmeliet and Yizhi Liu and Bernard Thienpont and Mieke Dewerchin and Guy Eelen and Xuri Li and Yonglun Luo and Peter Carmeliet",
note = "Copyright {\textcopyright} 2020 Elsevier Inc. All rights reserved.",
year = "2020",
month = apr,
doi = "10.1016/j.cmet.2020.03.009",
language = "English",
volume = "31",
pages = "862--877.e14",
journal = "Cell Metabolism",
issn = "1550-4131",
publisher = "Cell Press",
number = "4",

}

RIS

TY - JOUR

T1 - Single-Cell RNA Sequencing Maps Endothelial Metabolic Plasticity in Pathological Angiogenesis

AU - Rohlenova, Katerina

AU - Goveia, Jermaine

AU - García-Caballero, Melissa

AU - Subramanian, Abhishek

AU - Kalucka, Joanna

AU - Treps, Lucas

AU - Falkenberg, Kim D

AU - de Rooij, Laura P M H

AU - Zheng, Yingfeng

AU - Lin, Lin

AU - Sokol, Liliana

AU - Teuwen, Laure-Anne

AU - Geldhof, Vincent

AU - Taverna, Federico

AU - Pircher, Andreas

AU - Conradi, Lena-Christin

AU - Khan, Shawez

AU - Stegen, Steve

AU - Panovska, Dena

AU - De Smet, Frederik

AU - Staal, Frank J T

AU - Mclaughlin, Rene J

AU - Vinckier, Stefan

AU - Van Bergen, Tine

AU - Ectors, Nadine

AU - De Haes, Patrik

AU - Wang, Jian

AU - Bolund, Lars

AU - Schoonjans, Luc

AU - Karakach, Tobias K

AU - Yang, Huanming

AU - Carmeliet, Geert

AU - Liu, Yizhi

AU - Thienpont, Bernard

AU - Dewerchin, Mieke

AU - Eelen, Guy

AU - Li, Xuri

AU - Luo, Yonglun

AU - Carmeliet, Peter

N1 - Copyright © 2020 Elsevier Inc. All rights reserved.

PY - 2020/4

Y1 - 2020/4

N2 - Endothelial cell (EC) metabolism is an emerging target for anti-angiogenic therapy in tumor angiogenesis and choroidal neovascularization (CNV), but little is known about individual EC metabolic transcriptomes. By single-cell RNA sequencing 28,337 murine choroidal ECs (CECs) and sprouting CNV-ECs, we constructed a taxonomy to characterize their heterogeneity. Comparison with murine lung tumor ECs (TECs) revealed congruent marker gene expression by distinct EC phenotypes across tissues and diseases, suggesting similar angiogenic mechanisms. Trajectory inference predicted that differentiation of venous to angiogenic ECs was accompanied by metabolic transcriptome plasticity. ECs displayed metabolic transcriptome heterogeneity during cell-cycle progression and in quiescence. Hypothesizing that conserved genes are important, we used an integrated analysis, based on congruent transcriptome analysis, CEC-tailored genome-scale metabolic modeling, and gene expression meta-analysis in cross-species datasets, followed by in vitro and in vivo validation, to identify SQLE and ALDH18A1 as previously unknown metabolic angiogenic targets.

AB - Endothelial cell (EC) metabolism is an emerging target for anti-angiogenic therapy in tumor angiogenesis and choroidal neovascularization (CNV), but little is known about individual EC metabolic transcriptomes. By single-cell RNA sequencing 28,337 murine choroidal ECs (CECs) and sprouting CNV-ECs, we constructed a taxonomy to characterize their heterogeneity. Comparison with murine lung tumor ECs (TECs) revealed congruent marker gene expression by distinct EC phenotypes across tissues and diseases, suggesting similar angiogenic mechanisms. Trajectory inference predicted that differentiation of venous to angiogenic ECs was accompanied by metabolic transcriptome plasticity. ECs displayed metabolic transcriptome heterogeneity during cell-cycle progression and in quiescence. Hypothesizing that conserved genes are important, we used an integrated analysis, based on congruent transcriptome analysis, CEC-tailored genome-scale metabolic modeling, and gene expression meta-analysis in cross-species datasets, followed by in vitro and in vivo validation, to identify SQLE and ALDH18A1 as previously unknown metabolic angiogenic targets.

U2 - 10.1016/j.cmet.2020.03.009

DO - 10.1016/j.cmet.2020.03.009

M3 - Journal article

C2 - 32268117

VL - 31

SP - 862-877.e14

JO - Cell Metabolism

JF - Cell Metabolism

SN - 1550-4131

IS - 4

ER -