Institut for Biomedicin

Lars Bolund

Single-cell exome sequencing and monoclonal evolution of a JAK2-negative myeloproliferative neoplasm

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Standard

Single-cell exome sequencing and monoclonal evolution of a JAK2-negative myeloproliferative neoplasm. / Hou, Yong; Song, Luting; Zhu, Ping; Zhang, Bo; Tao, Ye; Xu, Xun; Li, Fuqiang; Wu, Kui; Liang, Jie; Shao, Di; Wu, Hanjie; Ye, Xiaofei; Ye, Chen; Wu, Renhua; Jian, Min; Chen, Yan; Xie, Wei; Zhang, Ruren; Chen, Lei; Liu, Xin; Yao, Xiaotian; Zheng, Hancheng; Yu, Chang; Li, Qibin; Gong, Zhuolin; Mao, Mao; Yang, Xu; Yang, Lin; Li, Jingxiang; Wang, Wen; Lu, Zuhong; Gu, Ning; Laurie, Goodman; Bolund, Lars; Kristiansen, Karsten; Wang, Jian; Yang, Huanming; Li, Yingrui; Zhang, Xiuqing; Wang, Jun.

I: Cell, Bind 148, Nr. 5, 2012, s. 873-85.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Hou, Y, Song, L, Zhu, P, Zhang, B, Tao, Y, Xu, X, Li, F, Wu, K, Liang, J, Shao, D, Wu, H, Ye, X, Ye, C, Wu, R, Jian, M, Chen, Y, Xie, W, Zhang, R, Chen, L, Liu, X, Yao, X, Zheng, H, Yu, C, Li, Q, Gong, Z, Mao, M, Yang, X, Yang, L, Li, J, Wang, W, Lu, Z, Gu, N, Laurie, G, Bolund, L, Kristiansen, K, Wang, J, Yang, H, Li, Y, Zhang, X & Wang, J 2012, 'Single-cell exome sequencing and monoclonal evolution of a JAK2-negative myeloproliferative neoplasm', Cell, bind 148, nr. 5, s. 873-85. https://doi.org/10.1016/j.cell.2012.02.028

APA

Hou, Y., Song, L., Zhu, P., Zhang, B., Tao, Y., Xu, X., ... Wang, J. (2012). Single-cell exome sequencing and monoclonal evolution of a JAK2-negative myeloproliferative neoplasm. Cell, 148(5), 873-85. https://doi.org/10.1016/j.cell.2012.02.028

CBE

Hou Y, Song L, Zhu P, Zhang B, Tao Y, Xu X, Li F, Wu K, Liang J, Shao D, Wu H, Ye X, Ye C, Wu R, Jian M, Chen Y, Xie W, Zhang R, Chen L, Liu X, Yao X, Zheng H, Yu C, Li Q, Gong Z, Mao M, Yang X, Yang L, Li J, Wang W, Lu Z, Gu N, Laurie G, Bolund L, Kristiansen K, Wang J, Yang H, Li Y, Zhang X, Wang J. 2012. Single-cell exome sequencing and monoclonal evolution of a JAK2-negative myeloproliferative neoplasm. Cell. 148(5):873-85. https://doi.org/10.1016/j.cell.2012.02.028

MLA

Vancouver

Author

Hou, Yong ; Song, Luting ; Zhu, Ping ; Zhang, Bo ; Tao, Ye ; Xu, Xun ; Li, Fuqiang ; Wu, Kui ; Liang, Jie ; Shao, Di ; Wu, Hanjie ; Ye, Xiaofei ; Ye, Chen ; Wu, Renhua ; Jian, Min ; Chen, Yan ; Xie, Wei ; Zhang, Ruren ; Chen, Lei ; Liu, Xin ; Yao, Xiaotian ; Zheng, Hancheng ; Yu, Chang ; Li, Qibin ; Gong, Zhuolin ; Mao, Mao ; Yang, Xu ; Yang, Lin ; Li, Jingxiang ; Wang, Wen ; Lu, Zuhong ; Gu, Ning ; Laurie, Goodman ; Bolund, Lars ; Kristiansen, Karsten ; Wang, Jian ; Yang, Huanming ; Li, Yingrui ; Zhang, Xiuqing ; Wang, Jun. / Single-cell exome sequencing and monoclonal evolution of a JAK2-negative myeloproliferative neoplasm. I: Cell. 2012 ; Bind 148, Nr. 5. s. 873-85.

Bibtex

@article{703776214786419aaef179c0c081ee44,
title = "Single-cell exome sequencing and monoclonal evolution of a JAK2-negative myeloproliferative neoplasm",
abstract = "Tumor heterogeneity presents a challenge for inferring clonal evolution and driver gene identification. Here, we describe a method for analyzing the cancer genome at a single-cell nucleotide level. To perform our analyses, we first devised and validated a high-throughput whole-genome single-cell sequencing method using two lymphoblastoid cell line single cells. We then carried out whole-exome single-cell sequencing of 90 cells from a JAK2-negative myeloproliferative neoplasm patient. The sequencing data from 58 cells passed our quality control criteria, and these data indicated that this neoplasm represented a monoclonal evolution. We further identified essential thrombocythemia (ET)-related candidate mutations such as SESN2 and NTRK1, which may be involved in neoplasm progression. This pilot study allowed the initial characterization of the disease-related genetic architecture at the single-cell nucleotide level. Further, we established a single-cell sequencing method that opens the way for detailed analyses of a variety of tumor types, including those with high genetic complex between patients.",
keywords = "Clonal Evolution, Exome, Gene Expression Profiling, Genome, Human, High-Throughput Nucleotide Sequencing, Humans, Janus Kinase 2, Male, Middle Aged, Mutation, Myeloproliferative Disorders, Single-Cell Analysis, Thrombocythemia, Essential",
author = "Yong Hou and Luting Song and Ping Zhu and Bo Zhang and Ye Tao and Xun Xu and Fuqiang Li and Kui Wu and Jie Liang and Di Shao and Hanjie Wu and Xiaofei Ye and Chen Ye and Renhua Wu and Min Jian and Yan Chen and Wei Xie and Ruren Zhang and Lei Chen and Xin Liu and Xiaotian Yao and Hancheng Zheng and Chang Yu and Qibin Li and Zhuolin Gong and Mao Mao and Xu Yang and Lin Yang and Jingxiang Li and Wen Wang and Zuhong Lu and Ning Gu and Goodman Laurie and Lars Bolund and Karsten Kristiansen and Jian Wang and Huanming Yang and Yingrui Li and Xiuqing Zhang and Jun Wang",
note = "Copyright {\^A}{\circledC} 2012 Elsevier Inc. All rights reserved.",
year = "2012",
doi = "10.1016/j.cell.2012.02.028",
language = "English",
volume = "148",
pages = "873--85",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "5",

}

RIS

TY - JOUR

T1 - Single-cell exome sequencing and monoclonal evolution of a JAK2-negative myeloproliferative neoplasm

AU - Hou, Yong

AU - Song, Luting

AU - Zhu, Ping

AU - Zhang, Bo

AU - Tao, Ye

AU - Xu, Xun

AU - Li, Fuqiang

AU - Wu, Kui

AU - Liang, Jie

AU - Shao, Di

AU - Wu, Hanjie

AU - Ye, Xiaofei

AU - Ye, Chen

AU - Wu, Renhua

AU - Jian, Min

AU - Chen, Yan

AU - Xie, Wei

AU - Zhang, Ruren

AU - Chen, Lei

AU - Liu, Xin

AU - Yao, Xiaotian

AU - Zheng, Hancheng

AU - Yu, Chang

AU - Li, Qibin

AU - Gong, Zhuolin

AU - Mao, Mao

AU - Yang, Xu

AU - Yang, Lin

AU - Li, Jingxiang

AU - Wang, Wen

AU - Lu, Zuhong

AU - Gu, Ning

AU - Laurie, Goodman

AU - Bolund, Lars

AU - Kristiansen, Karsten

AU - Wang, Jian

AU - Yang, Huanming

AU - Li, Yingrui

AU - Zhang, Xiuqing

AU - Wang, Jun

N1 - Copyright © 2012 Elsevier Inc. All rights reserved.

PY - 2012

Y1 - 2012

N2 - Tumor heterogeneity presents a challenge for inferring clonal evolution and driver gene identification. Here, we describe a method for analyzing the cancer genome at a single-cell nucleotide level. To perform our analyses, we first devised and validated a high-throughput whole-genome single-cell sequencing method using two lymphoblastoid cell line single cells. We then carried out whole-exome single-cell sequencing of 90 cells from a JAK2-negative myeloproliferative neoplasm patient. The sequencing data from 58 cells passed our quality control criteria, and these data indicated that this neoplasm represented a monoclonal evolution. We further identified essential thrombocythemia (ET)-related candidate mutations such as SESN2 and NTRK1, which may be involved in neoplasm progression. This pilot study allowed the initial characterization of the disease-related genetic architecture at the single-cell nucleotide level. Further, we established a single-cell sequencing method that opens the way for detailed analyses of a variety of tumor types, including those with high genetic complex between patients.

AB - Tumor heterogeneity presents a challenge for inferring clonal evolution and driver gene identification. Here, we describe a method for analyzing the cancer genome at a single-cell nucleotide level. To perform our analyses, we first devised and validated a high-throughput whole-genome single-cell sequencing method using two lymphoblastoid cell line single cells. We then carried out whole-exome single-cell sequencing of 90 cells from a JAK2-negative myeloproliferative neoplasm patient. The sequencing data from 58 cells passed our quality control criteria, and these data indicated that this neoplasm represented a monoclonal evolution. We further identified essential thrombocythemia (ET)-related candidate mutations such as SESN2 and NTRK1, which may be involved in neoplasm progression. This pilot study allowed the initial characterization of the disease-related genetic architecture at the single-cell nucleotide level. Further, we established a single-cell sequencing method that opens the way for detailed analyses of a variety of tumor types, including those with high genetic complex between patients.

KW - Clonal Evolution

KW - Exome

KW - Gene Expression Profiling

KW - Genome, Human

KW - High-Throughput Nucleotide Sequencing

KW - Humans

KW - Janus Kinase 2

KW - Male

KW - Middle Aged

KW - Mutation

KW - Myeloproliferative Disorders

KW - Single-Cell Analysis

KW - Thrombocythemia, Essential

U2 - 10.1016/j.cell.2012.02.028

DO - 10.1016/j.cell.2012.02.028

M3 - Journal article

C2 - 22385957

VL - 148

SP - 873

EP - 885

JO - Cell

JF - Cell

SN - 0092-8674

IS - 5

ER -