Lars Bolund

Genetically different cell subpopulations in hydatidiform moles. A study of three cases by RFLP, flow cytometric, cytogenetic, HLA, and morphologic analyses

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

  • Lone Sunde
  • L O Vejerslev, J F Kennedy Instituttet, Danmark
  • Jørgen K Larsen, Finsen Instituttet, Rigshospitalet, Danmark
  • Ib Jarle Christensen, Finsen Instituttet, Rigshospitalet, Danmark
  • Hanna E Hansen, The University Institute of Forensic Genetics. Copenhagen, Danmark
  • Bent Mogensen, The Danish Cancer Society, Department of Immunoserology, Aarhus, Danmark
  • L Bolund
Most investigators find a good correlation between the morphologic and cytogenetic classification of hydatidiform moles (HM), but exceptions have been noted. We have examined three cases of HM, using chromosome marker analysis on cultured cells, human leukocyte antigen typing on cultured and uncultured tissue, and restriction fragment length polymorphism (RFLP) analysis and flow cytometry on uncultured cells. In one morphologically partial mole, only one cell population (triploid) was found and data obtained by the above-mentioned techniques were concordant. The other two moles, which were classified morphologically as complete, consisted of several cell subpopulations differing in DNA content. In both cases only one cell population was disclosed by cytogenetic investigation. In one case, the cytogenetic analysis indicated that the cultured cells were near triploid with paternal chromosomes exclusively, whereas RFLP analysis showed that maternal X chromosomal alleles were present in the mole. The present findings demonstrate that some HMs contain cellular subpopulations with differing DNA content. One explanation for discordance between cytogenetic and morphologic classification may thus be the detection of only one cell subpopulation when karyotyping.
OriginalsprogEngelsk
TidsskriftCancer Genetics and Cytogenetics
Vol/bind37
Nummer2
Sider (fra-til)179-92
Antal sider14
ISSN0165-4608
StatusUdgivet - 1989

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