Institut for Biomedicin

Lars Bolund

Effects of extra- and intracellular calcium concentration on DNA replication, lateral growth, and differentiation of human epidermal cells in culture

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

DOI

  • Peter K.A. Jensen
  • ,
  • Jan O.R. Nørgård, Aarhus Universitet
  • ,
  • Christian Knudsen
  • ,
  • Viggo Nielsen
  • ,
  • Lars Bolund

Variation in the extra and intracellular concentration of calcium ([Ca]e and [Ca]i) affected the3Hthymidine labeling pattern of sorted Sphase cells in human epidermal cultures. A lowering of [Ca]e resulted in retarded lateral growth but, unless [Ca]e was extremely low, caused an increase in the proportion of strongly labelled (rapidly cycling) Sphase cells. An increased desquamation of superficial cells due to a reduced cellular cohesiveness was also observed in low calcium medium. Thus, a lowering of [Ca]e might stimulate the proliferation of a pool of cycling cells destined for rapid terminal differentiation and tissue regeneration, whereas proliferation destined for lateral growth is inhibited. Attempts to decrease the [Ca]i with the calcium chelator quin-2 at low [Ca]e seemed to elevate the proportion of strongly labelled Sphase cells, whereas an increased [Ca]i obtained with the ionophore A23187 caused a dramatic decrease in the proportion of Sphase cells that showed strong3Hthymidine incorporation. This implies that variation in both [Ca]i and [Ca]e may play a role in the regulation of proliferation and differentiation, in keratinocytes.

OriginalsprogEngelsk
TidsskriftVirchows Archiv B Cell Pathology Including Molecular Pathology
Vol/bind59
Nummer1
Sider (fra-til)17-25
Antal sider9
ISSN0042-6431
DOI
StatusUdgivet - 1 dec. 1990

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