Institut for Biomedicin

Lars Bolund

CRISPR-C: circularization of genes and chromosome by CRISPR in human cells

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

CRISPR-C : circularization of genes and chromosome by CRISPR in human cells. / Møller, Henrik Devitt; Lin, Lin; Xiang, Xi; Petersen, Trine Skov; Huang, Jinrong; Yang, Luhan; Kjeldsen, Eigil; Jensen, Uffe Birk; Zhang, Xiuqing; Liu, Xin; Xu, Xun; Wang, Jian; Yang, Huanming; Church, George M; Bolund, Lars; Regenberg, Birgitte; Luo, Yonglun.

I: Nucleic Acids Research, Bind 46, Nr. 22, 14.12.2018, s. e131.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Møller, HD, Lin, L, Xiang, X, Petersen, TS, Huang, J, Yang, L, Kjeldsen, E, Jensen, UB, Zhang, X, Liu, X, Xu, X, Wang, J, Yang, H, Church, GM, Bolund, L, Regenberg, B & Luo, Y 2018, 'CRISPR-C: circularization of genes and chromosome by CRISPR in human cells', Nucleic Acids Research, bind 46, nr. 22, s. e131. https://doi.org/10.1093/nar/gky767

APA

Møller, H. D., Lin, L., Xiang, X., Petersen, T. S., Huang, J., Yang, L., Kjeldsen, E., Jensen, U. B., Zhang, X., Liu, X., Xu, X., Wang, J., Yang, H., Church, G. M., Bolund, L., Regenberg, B., & Luo, Y. (2018). CRISPR-C: circularization of genes and chromosome by CRISPR in human cells. Nucleic Acids Research, 46(22), e131. https://doi.org/10.1093/nar/gky767

CBE

Møller HD, Lin L, Xiang X, Petersen TS, Huang J, Yang L, Kjeldsen E, Jensen UB, Zhang X, Liu X, Xu X, Wang J, Yang H, Church GM, Bolund L, Regenberg B, Luo Y. 2018. CRISPR-C: circularization of genes and chromosome by CRISPR in human cells. Nucleic Acids Research. 46(22):e131. https://doi.org/10.1093/nar/gky767

MLA

Møller, Henrik Devitt o.a.. "CRISPR-C: circularization of genes and chromosome by CRISPR in human cells". Nucleic Acids Research. 2018, 46(22). e131. https://doi.org/10.1093/nar/gky767

Vancouver

Author

Møller, Henrik Devitt ; Lin, Lin ; Xiang, Xi ; Petersen, Trine Skov ; Huang, Jinrong ; Yang, Luhan ; Kjeldsen, Eigil ; Jensen, Uffe Birk ; Zhang, Xiuqing ; Liu, Xin ; Xu, Xun ; Wang, Jian ; Yang, Huanming ; Church, George M ; Bolund, Lars ; Regenberg, Birgitte ; Luo, Yonglun. / CRISPR-C : circularization of genes and chromosome by CRISPR in human cells. I: Nucleic Acids Research. 2018 ; Bind 46, Nr. 22. s. e131.

Bibtex

@article{74a8ae42232549a097a8559bb7df8670,
title = "CRISPR-C: circularization of genes and chromosome by CRISPR in human cells",
abstract = "Extrachromosomal circular DNA (eccDNA) and ring chromosomes are genetic alterations found in humans with genetic disorders. However, there is a lack of genetic engineering tools to recapitulate and study the biogenesis of eccDNAs. Here, we created a dual-fluorescence biosensor cassette, which upon the delivery of pairs of CRISPR/Cas9 guide RNAs, CRISPR-C, allows us to study the biogenesis of a specific fluorophore expressing eccDNA in human cells. We show that CRISPR-C can generate functional eccDNA, using the novel eccDNA biosensor system. We further reveal that CRISPR-C also can generate eccDNAs from intergenic and genic loci in human embryonic kidney 293T cells and human mammary fibroblasts. EccDNAs mainly forms by end-joining mediated DNA-repair and we show that CRISPR-C is able to generate endogenous eccDNAs in sizes from a few hundred base pairs and ranging up to 207 kb. Even a 47.4 megabase-sized ring chromosome 18 can be created by CRISPR-C. Our study creates a new territory for CRISPR gene editing and highlights CRISPR-C as a useful tool for studying the cellular impact, persistence and function of eccDNAs.",
author = "M{\o}ller, {Henrik Devitt} and Lin Lin and Xi Xiang and Petersen, {Trine Skov} and Jinrong Huang and Luhan Yang and Eigil Kjeldsen and Jensen, {Uffe Birk} and Xiuqing Zhang and Xin Liu and Xun Xu and Jian Wang and Huanming Yang and Church, {George M} and Lars Bolund and Birgitte Regenberg and Yonglun Luo",
year = "2018",
month = dec,
day = "14",
doi = "10.1093/nar/gky767",
language = "English",
volume = "46",
pages = "e131",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "22",

}

RIS

TY - JOUR

T1 - CRISPR-C

T2 - circularization of genes and chromosome by CRISPR in human cells

AU - Møller, Henrik Devitt

AU - Lin, Lin

AU - Xiang, Xi

AU - Petersen, Trine Skov

AU - Huang, Jinrong

AU - Yang, Luhan

AU - Kjeldsen, Eigil

AU - Jensen, Uffe Birk

AU - Zhang, Xiuqing

AU - Liu, Xin

AU - Xu, Xun

AU - Wang, Jian

AU - Yang, Huanming

AU - Church, George M

AU - Bolund, Lars

AU - Regenberg, Birgitte

AU - Luo, Yonglun

PY - 2018/12/14

Y1 - 2018/12/14

N2 - Extrachromosomal circular DNA (eccDNA) and ring chromosomes are genetic alterations found in humans with genetic disorders. However, there is a lack of genetic engineering tools to recapitulate and study the biogenesis of eccDNAs. Here, we created a dual-fluorescence biosensor cassette, which upon the delivery of pairs of CRISPR/Cas9 guide RNAs, CRISPR-C, allows us to study the biogenesis of a specific fluorophore expressing eccDNA in human cells. We show that CRISPR-C can generate functional eccDNA, using the novel eccDNA biosensor system. We further reveal that CRISPR-C also can generate eccDNAs from intergenic and genic loci in human embryonic kidney 293T cells and human mammary fibroblasts. EccDNAs mainly forms by end-joining mediated DNA-repair and we show that CRISPR-C is able to generate endogenous eccDNAs in sizes from a few hundred base pairs and ranging up to 207 kb. Even a 47.4 megabase-sized ring chromosome 18 can be created by CRISPR-C. Our study creates a new territory for CRISPR gene editing and highlights CRISPR-C as a useful tool for studying the cellular impact, persistence and function of eccDNAs.

AB - Extrachromosomal circular DNA (eccDNA) and ring chromosomes are genetic alterations found in humans with genetic disorders. However, there is a lack of genetic engineering tools to recapitulate and study the biogenesis of eccDNAs. Here, we created a dual-fluorescence biosensor cassette, which upon the delivery of pairs of CRISPR/Cas9 guide RNAs, CRISPR-C, allows us to study the biogenesis of a specific fluorophore expressing eccDNA in human cells. We show that CRISPR-C can generate functional eccDNA, using the novel eccDNA biosensor system. We further reveal that CRISPR-C also can generate eccDNAs from intergenic and genic loci in human embryonic kidney 293T cells and human mammary fibroblasts. EccDNAs mainly forms by end-joining mediated DNA-repair and we show that CRISPR-C is able to generate endogenous eccDNAs in sizes from a few hundred base pairs and ranging up to 207 kb. Even a 47.4 megabase-sized ring chromosome 18 can be created by CRISPR-C. Our study creates a new territory for CRISPR gene editing and highlights CRISPR-C as a useful tool for studying the cellular impact, persistence and function of eccDNAs.

U2 - 10.1093/nar/gky767

DO - 10.1093/nar/gky767

M3 - Journal article

C2 - 30551175

VL - 46

SP - e131

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 22

ER -