Institut for Biomedicin

Lars Bolund

Cell kinetically defined subpopulations of cultured human epidermal keratinocytes differ with respect to cell size and staining pattern with antikeratin antibodies

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

  • P. K.A. Jensen
  • ,
  • C. Knudsen
  • ,
  • S. Pedersen
  • ,
  • L. Bolund

By combining the technique of fluorescence-activated cell sorting with 3H-thymidine labelling and autoradiography a dramatic heterogeneity in the labelling intensity of S-phase cells in human skin epithelial cultures has been revealed. Cell kinetic studies have indicated the existence of subpopulations of cycling cells that seem to differ with respect to their rate of DNA replication. Studies with growth stimulators and inhibitors as well as analysis of epidermal regeneration in vitro have suggested that these subpopulations are differentially involved in cell renewal and early differentiation of the epidermal keratinocyte. In the present study it is shown that the cell kinetically defined subpopulations of proliferating keratinocytes differ with respect to early differentiation markers of the keratinocyte: Both mean cell diameter and immunoreactivity with monoclonal antikeratin antibodies vary with the labelling intensity of sorted S-phase cells. The AE1 antikeratin antibody preferentially stains small and medium-sized, strongly DNA labelled cells, whereas the AE2 antibody mainly stains large unlabelled and weakly labelled cells. Small cells are never stained by AE2. A subpopulation of very small, unlabelled S-phase cells have a characteristic morphology and some of these cells are unstained with all AE antibodies. The functional inter-relationship of these subpopulations is unclear, but the results may suggest a hierarchy of cycling cell populations in human epidermal cultures: A fraction of very small, immature, unlabelled S-phase cells may be considered candidates of stem cells; strongly labelled S-phase cells are intermediary in degree of maturation, whereas the large unlabelled and the weakly labelled S-phase cells seem to be the most mature cells. The experiments reported here support the idea of differential roles for the cell kinetically defined subpopulations of proliferating keratinocytes in cell renewal, commitment, and differentiation.

Sider (fra-til)307-321
Antal sider15
StatusUdgivet - 1 jan. 1987

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