Institut for Biomedicin

Lars Bolund

A flow cytometric competition technique for measuring interaction of LDL with cellular LDL-receptors applied to patients with mutant (Arg3500-->Gln) apolipoprotein B

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

  • F Heath, Department of Medicine and Cardiology, Aarhus Amtssygehus University Hospital, Denmark.
  • ,
  • T G Jensen
  • P S Hansen
  • ,
  • L G Jensen
  • ,
  • H Vissinger
  • ,
  • S K Moestrup
  • S Pedersen
  • ,
  • S Kølvraa
  • ,
  • L Bolund
  • O Faergeman

We report our experience with a method to evaluate binding and uptake in cells of low density lipoprotein (LDL) from heterozygous patients with familial defective apolipoprotein B-100 (FDB-LDL) and LDL from normolipidemic subjects (nonFDB-LDL). The method is based on competition for binding/uptake in Epstein-Barr Virus (EBV)-transformed lymphocytes or COS cells overexpressing an LDL-receptor transgene between fluorescently labeled LDL and the unlabeled LDL of interest, and measurements are by flow cytometry. With EBV-lymphoblasts, the ability of FDB-LDL to displace fluorescent LDL ("Dil"-LDL) from cells at 4 degrees C (binding) was reduced to approximately 1/3 of normal. Displacement of "Dil"-LDL by FDB-LDL from cells at 20 degrees C (binding/uptake) was reduced to less than 1/2 of normal. Similar results were obtained with COS cells. Freezing of serum to -80 degrees C for 24 hours did not affect results, and we could discriminate between binding/uptake of FDB-LDL and nonFDB-LDL prepared from serum that had been stored at -80 degrees C for three months.

OriginalsprogEngelsk
TidsskriftZeitschrift fuer Gastroenterologie
Vol/bind34 Suppl 3
Sider (fra-til)21-4
Antal sider4
ISSN0044-2771
StatusUdgivet - jun. 1996

Se relationer på Aarhus Universitet Citationsformater

ID: 103467236