Curriculum Vitae for Lars Bolund


Born on March 20. 1944, Stockholm, Sweden.

Danish Citizen: October 31. 1983.

Married to Dorte Bolund. Three children and one stepchild.


Education:

1963: Matriculate (studentexamen), Kungsholmens Läroverk, Stockholm, with the highest set of marks in Sweden.

1965: Bachelor of Medicine (Med.Kand.) at Karolinska Institutet, Stockholm.

1971: Doctorate in Medicine (DMSc; Med.Dr.). The thesis was awarded top mark (Berömlig) and led to immediate appointment as Docent in Medical Cell Research and Genetics at Karolinska Institutet, Stockholm.

1976: Graduation in Medicine (MD; Läkarexamen) at Karolinska Institutet, Stockholm.


Supplementary education:

1970-1971: General course and examination in Physical Chemistry at the Royal Institute of Technology, Stockholm.

18 tutorials and practical research courses in mathematics, medical computing and data handling, quantitative cytochemistry, immunochemistry, cytometry, cell and molecular biology, molecular genetics etc. in Sweden, England,
Holland,France, U.S.A. and Denmark.


Positions:

01.05.1964-30.04.1965: Amanuensis extraordinary, Institute for Medical Cell Research and Genetics, Karolinska Institutet, Stockholm.

01.05.1965-31.03.1971: Third amanuensis, same institute.

01.05.1971-31.08.1971: Research assistant, same institute.

01.09.1971-30.06.1972: Post-doctoral scientist at Department of Molecular Biology, Chester Beatty Cancer Research Institute, London, on a scholarship from the British Royal Society.

01.07.1972-31.01.1974: Employed on a research grant from the Swedish Cancer Society working at Karolinska Institutet, Stockholm, Chester Beatty Cancer Research.Institute, London, and Portsmouth Polytechnic.

01.02.1974-31.01.1976: Scientist of the Swedish Cancer Society in the field of "Cancer research – in particular cell genetics" at Institute for Medical Cell Research and Genetics, Karolinska Institutet, Stockholm.

01.02.1976: Professor of Clinical Genetics, Institute of Human Genetics, Aarhus University.

13.01.1984: Lifetime Appointment as Professor by the Queen of Denmark.

01.09.1987-30.09.1988: Research Professor of the Aarhus University Research Fund.

01.10.1992-31.07.1993: Visiting Professor at Division of Molecular Cytometry, UCSF School of Medicine, San Francisco.

01.02.1995-31.01.2001: Leader of Institute of Human Genetics, Aarhus University.

01.08.1998-09.09.1999: Senior Advisor, Human Genome Centre, Chinese Academy of Sciences.

09.09.1999: Senior Advisor and Adjunct Professor, Beijing Genomics Institute, BGI/HuaDa JiYin.

01.04.2001-31.03.2002: Visiting Scholar at Genome Centre, University of Washington, Seattle.

09.09.2007: Senior Advisor and Adjunct Professor, BGI/HuaDa JiYin Shenzhen.

19.02.2010: Visiting Professor of Wenzhou Medical College.

01.05.2011: Adjunct Professor, Institute of Biology, Copenhagen University.


Teaching:

1.Pre-graduate:
Since 01.02.1976: Lectures, tutorials and examinations in basic genetics, clinical genetics and medical statistics at the Medical and Odontological School, University of Aarhus.

2.Post-graduate:
Organization of 3 practical research courses in cell genetics and methods of cell biology, and 10 courses in clinical genetics for the National Health Service.

Organization of 2 theoretical and practical courses in molecular and clinical genetics in Beijing, 1988, and function as Senior Advisor for Chinese research students in Beijing and Aarhus in relation to research projects under the Sino-Danish Scientific and Technological Cooperation Programme. Teaching at a large number of post-graduate courses in Denmark and abroad (including China).


Research supervision:

Supervision of more than 30 pre-graduate researchers, Ph.D. students and doctorate candidates from Medical as well as Natural Science Faculties in Sweden, Denmark, Poland and China (more than 15 Ph.D.-students from China only).


Scientific evaluation:

Chairman/member of numerous evaluation committees regarding Ph.D.-exams, doctorates, research positions and professorships in Denmark, Finland, Norway, Sweden and U.S.A.

Evaluation (since 1983) of research grant applications for the Danish Medical Research Council, Novo's Fund and the European Commission.

Referee for a number of international scientific journals.


Research politics:

Member of the Board of the Danish Society for Medical Genetics (1978-1982) and its chairman (1986-1990).

Member of the Danish Medical Research Council (1983-1987).

Chairman of ad hoc Research Budgetary Committee of the Medical Faculty, Aarhus University (1984).

Chairman of Scientific Evaluation Committee for Institute of Medical Biochemistry, Aarhus University (1986).

Member of the Parliamentary Board of Technology Assessment (1986-1988).

Member of the Danish Council of Gene Technology (1987-1991).

Member of the Danish Council of Ethics (1988-1991).

Member of the Medical Faculty Council, Aarhus University (1988-1992).

Member of the Human Genome Organization (HUGO) since 1988.

Member of the EC Working Party for the Human Genome Analysis Programme and Chairman for a Study Group on Advanced Genetic Technologies (1988-1990).

Member of the EC Committee of Advisory Nature on Human Genome Analysis (CAN-HUG) (1990-1993).

Member of the EC Committee of Advisory Nature on the Biomedical Research Programme (CAN-MED) (1991-1993).

Chairman of the EC Ethical Committee on Human Embryo Research (1991-1993).

Member of EC Field Review on Small Model Genomes in 1994.

Member of the EC CREST ad hoc Working Group Biomedicine and Health (1994-1996).

Member of the Finance Committee and Scientific Advisory Committee of the International Society for Analytical Cytology (ISAC) (1991-1993).

Vice-president of the Permanent Committee of the International Congress of Human Genetics (1991-1996).

Member of the Board of the Danish Cancer Society (1992-2001).

Member of the Executive Committee of the Danish Cancer Society (1993-2001).

Member of the Medical and Natural Science Committee of Novo´s Fund (1993-2002).

Member of the Board of James D. Watson Institute of Genome Science and BGI/HuaDa JiYin, Hangzhou, since 2003.

Member of the Board of EFBIC (European Focus on Biotechnology in China) since 2003.

Member of the Board of Eagles (European Action for Globalization of Life Sciences) since 2007


Miscellanea:

Scientific Editorial Assistant for Experimental Cell Research, Academic Press, N.Y. (1971-1976).

Pilot Licence (1977).

Swedish Commercial Flight Licence (B-certificate) and Danish Instrument Flight Rating (1981).

Expert on DNA examinations for the Danish Medico-Legal Council since 1989.

Organization of 8 international meetings, symposia and workshops in Scandinavia (1985- ).


Clinical activities:

Development of a Division of Clinical Genetics at the Institute of Human Genetics, financed through its diagnostic and councelling services and in 1995 transferred to the national health system with 4 physicians, 20 technicians and a budget of app. 10 million D.Kr. Genetic councelling of patients and families referred from hospitals and general practitioners in Northern Jutland (1978-1985).


Special Honours:

1979 The Odd Fellow's Research Prize (together with professor A.J.Therkel¬sen).

1987 Research Prize of "The Foundation of 1870".

1991 Consul-general Ernst Carlsen's Honorary Prize.

1996 The August Krogh Prize. Award of the Danish Medical Society and NOVO’s Fund.

1998 Honorary Professor of the Chinese Academy of Sciences (Human Genome Center).

2003 Honorary Professor of the Chinese Academy of Medical Sciences (Peking Union Medical College).

2006 Honorary Professor of Beijing Institute of Genomics, Chinese Academy of Sciences.

2008 Honorary Professor of Hainan Medical College.


1985 Knight of the Royal Danish Order of Dannebrog.

1998 Knight of the 1st class of the Royal Danish Order of Dannebrog.


2009 Honoured with the Chinese National Friendship Award in connection with the 60th Anniversary of the People´s Republic of China.


Publications:

340 scientific articles (including 15 in Cell, Science, and Nature Series), database submissions and patents in the fields of genome/gene structure and function in cell biology and clinical genetics, molecular cell pathology of complex diseases, somatic cell evolution, development of genetically designed animal models of chronic disease processes, regenerative medicine and genetic therapy (see separate list of publications).



LIST OF PUBLICATIONS: (Lars Bolund)January, 2013


1.Intracellular distribution of histone-like proteins in Tetrahymena pyriformis.

L. Bolund & N.R. Ringertz.

Exptl. Cell Res. 44 (1966) 606-613.


2.Isolation and chemical composition of macronuclei from tetrahymena.

N.R. Ringertz, L. Bolund & L.E. DeBault.

Exptl. Cell Res. 45 (1967) 519-532.


3.Changes in the cytochemical properties of erythrocyte nuclei reactivated by cell fusion.

L. Bolund, N.R. Ringertz & H. Harris.

J. Cell Science 4 (1969) 71-87.


4.Actinomycin binding capacity of deoxyribonucleoprotein.

N.R. Ringertz & L. Bolund.

Biochim. Biophys. Acta 174 (1969) 147-154.


5.Actinomycin binding of normal and phytohaemagglutinin stimulated lymphocytes.

Z. Darzynkiewicz, L. Bolund & N.R. Ringertz.

Exptl. Cell Res. 55 (1969) 120-123.


6."Activation" of hen erythrocyte deoxyribonucleoprotein.

N.R. Ringertz & L. Bolund.

Exptl. Cell Res. 55 (1969) 205-214.


7.Cytochemical characterization of deoxyribonucleoprotein in individual cell nuclei.

R. Rigler, D. Killander, L. Bolund & N.R. Ringertz.

Exptl. Cell Res. 55 (1969) 215-224.


8.Growth of hen erythrocyte nuclei undergoing reactivation in heterokaryons.

L. Bolund, Z. Darzynkiewicz & N.R. Ringertz.

Exptl. Cell Res. 56 (1969) 406-410.


9.Actinomycin binding properties of stimulated human lymphocytes.

N.R. Ringertz, Z. Darzynkiewicz & L. Bolund.

Exptl. Cell Res. 56 (1969) 411-417.


10.Nucleoprotein changes and initiation of RNA synthesis in PHA stimulated lymphocytes.

Z. Darzynkiewicz, L. Bolund & N.R. Ringertz.

Exptl. Cell Res. 56 (1969) 418-424.


11.Structural changes in the deoxyribonucleoprotein (DNP) complex of leukocytes from patients with infectious mononucleosis.

L. Bolund, G. Gahrton, D. Killander, R. Rigler & B. Wahren.

Blood 35: 3 (1970) 322-332.



12.Cell concentration and the staining properties of nuclear deoxyribonucleoprotein.

L. Bolund, Z. Darzynkiewicz & N.R. Ringertz.

Exptl. Cell Res. 62 (1970) 76-89.


13.Actinomycin D binding to isolated deoxyribonucleoprotein and intact cells.

L. Bolund.

Exptl. Cell Res. 63 (1970) 171-188.


14.Acridine orange binding of intracellular nucleic acids in fixed cells in relation to cell growth.

N.R. Ringertz, L. Bolund & Z. Darzynkiewicz.

Exptl. Cell Res. 63 (1970) 233-238.


15.Chromatin changes during erythropoiesis.

A.M. Kernell, L. Bolund & N.R. Ringertz.

Exptl. Cell Res. 65 (1971) 1-6.


16.Cytochemical properties of chromatin and nuclei in relation to genome activation and repression.

L. Bolund. Thesis.

Tryckeri Balder (1971) Stockholm.


17.Detection of human and chick nuclear antigens in nuclei of chick erythrocytes during reactivation in heterokaryons with HeLa cells.

N.R. Ringertz, S.A. Carlsson, T. Ege & L. Bolund.

Proc. Natl. Acad. Sci. 68 (1971) 3228-3232.


18.Influence of cell density on the acridine orange binding to deoxyribonucleoprotein complex in leucocytes from patients with infectious mononucleosis and acute leukemia.

L. Bolund, L.E. DeBault, G. Foley, G. Gahrton, D. Killander, W.C. Moloney, R. Rigler & B. Wahren.

Acta Haemat. (Basel) 48 (1972) 227-233.


19.X-ray studies of nucleoproteins depleted of lysine rich histones.

E.M. Bradbury, H.V. Molgaard, R.M. Stephens, L. Bolund & E.W. Johns.

Eur. J. Biochem. 31 (1972) 474-482.


20.The selective extraction of histone fractions from deoxyribonucleoprotein.

L. Bolund & E.W. Johns.

Eur. J. Biochem. 35 (1973) 546-553.


21.The nucleus during avian erythroid differentiation.

N.R. Ringertz & L. Bolund.

In The Cell Nucleus Vol. III. Ed. H Busch. Academic Press, N.Y. (1974) 417-446.


22.Reactivation of chick erythrocyte nuclei by somatic cell hybridization.

N.R. Ringertz & L. Bolund.

In International Review of experimental Pathology Vol. XIII, Academic Press, N.Y. (1974) 83-116.


23.Chick erythrocyte chromatin as a template for RNA synthesis in vitro.

L. Bolund & E.W. Johns.

Eur. J. Biochem. 40 (1974) 591-598.


24.The kinetics of protein uptake by chick erythrocyte nuclei during reactivation in chick-mammalian heterokaryons.

R. Appels, L. Bolund, S. Goto & N.R. Ringertz.

Exptl. Cell Res. 85 (1974) 182-190.


25.Biochemical analysis of reactivated chick erythrocyte nuclei isolated from chick-HeLa heterokaryons.

R. Appels, L. Bolund & N.R. Ringertz.

J. Mol. Biol. 87 (1974) 339-355.


26.Synthesis of oviduct nuclear and chromatin proteins during steroid induced differentia-tion.

K. Hemminki & L. Bolund.

Cell Differentiation 3 (1975) 347-359.


27.Gentekniken och den framtida medicinen.

L. Bolund.

IVA-Rapport nr. 95, Stockholm (1976) 37-46.


28.Acridine orange binding to chromatin of individual cells and nuclei under different staining conditions. I. Binding capacity of chromatin.

R. Liedemann & L. Bolund.

Exptl. Cell Res. 101 (1976) 164-174.


29.Acridine orange binding to chromatin of individual cells and nuclei under different staining conditions. II. Thermodenatu¬ration of chromatin.

R. Liedemann & L. Bolund.

Exptl. Cell Res. 101 (1976) 175-183.


30.The ploidy classes of adult mouse liver cells. A methodolo¬gical study with flow cytometry and cell sorting.

V. Digernes & L. Bolund.

Virchows Arch. B. Cell Path. 32 (1979) 1-10.


31.Epigenetisk manipulation. Programmering af arvemassen.

L. Bolund.

Ugeskrift for læger 139 (1977) 900-902.


32.Origin of triploidy in spontaneous abortuses.

J.G. Lauritsen, L. Bolund, U. Friedrich & A.J. Therkelsen.

Ann. Hum. Genet., London 43 (1979) 1-6.


33.DNA synthesis in mouse epidermis. Labelled and unlabelled basal cells in S phase after administration of tritiated thymidine.

O.P. F. Clausen, E. Thorud & L. Bolund.

Virchows Arch. B. Cell Path. 34 (1980) 1-11.


34.Flow sorting of chromatin.

L. Bolund.

Acta Path. Microbiol. Scand. Suppl. 274 (1981) 260-267.


35.DNA synthesis in mouse epidermis. S phase cells that remain unlabelled after pulse labelling with DNA precursors progress slowly through S.

O.P.F. Clausen, K. Elgjo, B. Kirkhus, S. Pedersen & L. Bolund.

J. Invest. Dermatol. 81 (1983) 545-549.


36.Morphological and functional differentiation in epithelial cultures obtained from human skin explants.

P.K.A. Jensen, J.O.R. Nørgård, S. Pedersen & L. Bolund.

Virchows Arch. B. Cell Path. 44 (1983) 305-322.


37.Subpopulations of slowly cycling cells in S and G2 phase in mouse epidermis.

O.P.F. Clausen, E. Aarnaes, B. Kirkhus, S. Pedersen & L. Bolund.

Cell Tissue Kinet. 17 (1984) 351-365.


38.Gensplejsning.

L. Bolund.

In Etiske sider af gensplejsnings-, ægtransplantations-, fosterundersøgelses- og inseminationsteknikker. Indenrigsministeriet (1984) 18-25.


39.Anonymous X-chromosomal probes revealing DNA polymorphisms one of which is a deletion of more than 3 kb.

P. Ahrens, H. Albertsen, S. Riis Vestergaard, L. Bolund & T.A. Kruse.

Cytogenetics and Cell Genetics 40 (1985) 567.


40.Basal cell subpopulations and cell cycle kinetics in human epidermal explant cultures.

P.K.A. Jensen, S. Pedersen & L. Bolund.

Cell Tissue Kinet. 18 (1985) 201-215.


41.DNA synthesis rate changes during the S phase in mouse epidermis.

O.P.F. Clausen, B. Kirkhus, K. Elgjo, S. Pedersen & L. Bolund.

Cell Tissue Kinet. 18 (1985) 445-455.


42.Changes in basal cell subpopulations and tissue differentia¬tion in human epidermal cultures treated with epidermal growth factor and cholera toxin.

P.K.A. Jensen, J.O.R. Nørgård & L. Bolund.

Virchows Arch. B. Cell Path. 49 (1985) 325-340.


43.Changes in proliferating cell subpopulations and mitotic activity in human epidermal cultures treated with epithelial growth inhibitors.

P.K.A. Jensen & L. Bolund.

J. Invest. Dermatol. 86 (1986) 46-50.


44.Recent research on human DNA-polymorphism.

L. Bolund.

In Advances in Forensic Haemogenetics, Eds. B. Brinkmann & K. Henningsen.

Springer Verlag (1986) 195.


45.DNA probes for linkage analysis of X-linked diseases: Localization of the disease locus for anhidrotic ectodermal dysplasia.

L. Bolund, P. Ahrens, H. Albertsen, P.K.A. Jensen, T.A. Kruse & S. Kølvraa.

Clinical Genetics 29 (1986) 458.


46.Close linkage between X-linked ectodermal dysplasia and a cloned DNA sequence detecting a two-allele restriction fragment length polymorphism in the region Xp11-q12.

S. Kølvraa, T.A. Kruse, P.K.A. Jensen, K.H. Linde, S.R. Vestergaard & L. Bolund.

Human Genetics 74 (1986) 284-287.


47.An improved method for labelling of DNA probes by nick translation.

J. Koch, S. Kølvraa & L. Bolund.

Nucl. Acid Res. 17 (1986) 7132.


48.The use of biotinylated hybridization probes for routine analysis of unique sequences in human DNA.

J. Koch, N. Gregersen, S. Kølvraa & L. Bolund.

Nucl. Acid Res. 17 (1986) 7133.


49.Dual parameter flow cytometric measurement of DNA-content and lectin binding in human bladder tumours.

T.F. Ørntoft, L. Bentsen, H.S. Poulsen, S.E. Pedersen, K. Wulf, S. Pedersen & L. Bolund.

Lectins vol. 5 (1986) 545-550.


50.Menneskets arvemasse med og uden genteknologi.

L. Bolund.

Forskningspolitik 3 (1986) 7-17.


51.Biotinylated DNA probes for hybridization analysis of DNA on membranes and in situ.

J. Koch, S. Kølvraa, N. Gregersen, K. Bruun Petersen & L. Bolund.

Ann. Univ. Sarav. Med. Suppl. 7 (1987) 182-185.


52.Improved methods for the detection of unique sequences in Southern blots of mammalian DNA by non-radioactive biotiny¬lated DNA hybridization probes.

N. Gregersen, J. Koch, S. Kølvraa, K. Bruun Petersen & L. Bolund.

Clin. Chem. Acta 169 (1987) 267-280.


53.Endogenous polyamines are intimately associated with highly condensed chromatin in vivo. A fluorescence cytochemical and immunocytochemical study of spermine and spermidine during the cell cycle and in reactivated nuclei.

D.M. Hougaard, L. Bolund, K. Fujiwara & L.-I. Larsson.

European Journal of Cell Biology 44 (1987) 151-155.


54.X-linked anhidrotic ectodermal dysplasia (EDA). Two point and multipoint linkage analysis using 7 polymorphic DNA markers.

K.D. MacDermot, S.H. Morgan, J.K. Cheshire, T.M. Wilson, I. Kaitila, A.-L. Söderholm, A. de la Chapelle, S. Kølvraa, L. Bolund & T.A. Kruse.

Cytogenetics and Cell Genetics 46 (1988) 653.


55.Sequence-dependent Effect of Camptothecin on Human Topoiso¬merase I DNA Cleavage.

E. Kjeldsen, S. Mollerup, B. Thomsen, B.J. Bonvén, L. Bolund & O. Westergaard.

J. Mol. Biol. 202 (1988) 333-342.


56.Characterization of a camptothecin resistent human DNA topoisomerase I.

E. Kjeldsen, B.J. Bonvén, T. Andoh, K. Ishii, K. Okada, L. Bolund & O. Westergaard.

J. Biol. Chem. 263 (1988) 3912-3916.


57.Low Ca2+ stripping of differentiating cell layers in human epidermal cultures: An in vitro model of epidermal regeneration.

P.K.A. Jensen & L. Bolund.

Exptl. Cell. Res. 175 (1988) 63-73.


58.Assignment of the human aminopeptidase N (peptidase E) gene to chromosome 15q13-qter.

T. Kruse, L. Bolund, J. Olsen, H. Sjöström & O. Norén.

FEBS letters 239 (1988) 305-308.


59.Detection of alpha-1-antitrypsin genotypes by analysis of amplified DNA sequences.

K.B. Petersen, S. Kølvraa, L. Bolund, G.B. Petersen, J. Koch & N. Gregersen.

Nucl. Acid Res. 16 (1988) 352.


60.Treatment of genomic DNA with T4 DNA ligase improves Southern blot analysis.

J. Koch, S. Kølvraa, M. Corneliusen, K.B. Petersen, N. Gregersen & L. Bolund.

Nucl. Acid Res. 16 (1988) 10387-10388.


61.The human lactase-phlorizin hydrolase gene is located on chromosome 2.

T. Kruse, L. Bolund, H. Sjöström, O. Norén, N. Montei & G. Semenza.

FEBS letters 240 (1988) 123-126.


62.Polymorphism and mapping of the gene encoding human aminopeptidase N.

T.A. Kruse, L. Bolund, L. Larsen, J. Olsen, H. Sjöström & O. Norén.

Cytogenetics and Cell Genetics 51 (1989) 1026.


63.Genetically different cell subpopulations in hydatidiform moles: A study of three cases by RFLP, flow cytometric, cytogenetic, HLA, and morphologic analysis.

L. Sunde, L.O. Vejerslev, J.K. Larsen, I.J. Christensen, H.E. Hansen, B. Mogensen & L. Bolund.

Cancer Genet. Cytogenet. 37 (1989) 179-192.


64.Detection of point mutations in amplified single copy genes by biotin-labelled oligonucleotides: Diagnosis of variants of alpha-1-antitrypsin.

N. Gregersen, V. Winter, K.B. Petersen, J. Koch, S. Kølvraa, N. Rüdiger, E.-M. Heinsvig & L. Bolund.

Clin. Chem. Acta 182 (1989) 151-164.


65.Cell kineticaly defined subpopulations of cultured human epidermal keratinocytes differ with respect to cell size and staining pattern with antikeratin antibodies. S_phase cells with low incorporation of DNA precursors are either small and immature or large and well differentiated.

P.K.A. Jensen, C. Knudsen, S. Pedersen & L. Bolund.

Epithelia 1:4 (1989) 307-321.


66.X-linked anhidrotic ectodermal dysplasia (EDA). Multipoint linkage analysis.

T.A. Kruse, S. Kølvraa, L. Bolund, I. Kaitila, A.-L. Söder¬holm, A. de la Chapelle & K.D.MacDermot.

Cytogenetics and Cell Genetics 51 (1989) 1026.


67.Oligonucleotide-priming methods for the chromosome-specific labelling of a-satellite DNA in situ.

J. Koch, S. Kølvraa, K.B. Petersen, N. Gregersen, & L. Bolund.

Chromosoma 98 (1989) 259-265.


68.Mapping of the human lactase-phlorizin hydrolase gene to chromosome 2.

T.A. Kruse, L. Bolund, A. Byskov, H. Sjöström, O. Norén,

N. Mantei & G. Semenza.

Cytogenetics and Cell Genetics 51 (1989) 1026.


69.Ethics and medical genetics in Denmark.

A.J. Therkelsen, L. Bolund & V. Mortensen.

In Ethics and Medical Genetics, Ed. J. Fletcher. Springer Verlag (1989) 141-155.


70.Proliferation and differentiation of cultured epidermal cells from patients with X-linked ichthyosis and ichthyosis vulgaris.

P.K.A. Jensen, F.H. Herrmann, J. Hadlich & L. Bolund.

Acta. Dermatovenerol. 70 (1990) 99-104.


71.A case of 46,XX,r(X)(p1q1) diagnosed by in situ hybridization.

J. Koch, S. Kølvraa, N. Hobolt, G.B. Petersen, H.F. Willard, J.S. Waye, N. Gregersen, & L. Bolund.

Clinical Genetics 37 (1990) 216-220.


72.In situ hybridization analysis of isodicentric X-chromosomes with short arm fusion.

J. Koch, S. Kølvraa, N. Gregersen, K. Rasmussen, J.M. Hertz & L. Bolund.

Clinical Genetics 37 (1990) 450-455.


73.Effects of extra- and intracellular calcium concentration on DNA replication, lateral growth and differentiation of human epidermal cells in culture.

P.K.A. Jensen, J.O.R. Nørgaard, C. Knudsen, V. Nielsen & L. Bolund.

Virchows Arch. B Cell Pathol. 59 (1990) 17-25.


74.High frequency transfection of cultured human epidermal basal cells that differentiate to form a multilayered tissue.

T. Jensen, P.K.A. Jensen, J.O.R. Nørgård & L. Bolund.

Exptl. Cell Res. 189 (1990) 163-168.


75.Hvad laver Institut for Human Genetik?

L. Bolund & A.J. Therkelsen.

Medicus 6 (1990) 7-9.


76.Synthetic epidermal pentapeptide and related growth regulatory peptides inhibit proliferation and enhance differentiation in primary and regenerating cultures of human epidermal keratinocytes.

P.K.A. Jensen, K. Elgjo, O.D. Laerum & L. Bolund.

J. Cell Science 97 (1990) 51-58.


77.A three allele Taq1 polymorphism at TOP1 gene.

L. Sunde, E. Kjeldsen, T. Andoh, J.D. Keene & L. Bolund.

Nucl. Acid Res. 18 (1990) 5919.


78.Molecular characterization of medium-chain acyl - CoA dehydrogenase (MCAD) deficiency: identification of a Lys329 to Glu mutation in the MCAD gene, and expression of inactive mutant enzyme protein in E. Coli.

N. Gregersen, B.S. Andresen, P. Ross, V. Winter, N. Rüdiger, S. Engst, E. Chri¬stensen, D. Kelly, A.W. Strauss, S. Kølvraa L. Bolund & S. Ghisla.

Human Genetics 86 (1991) 545-551.


79.Application of fluorescence in situ hybridization techniques in clinical genetics: Use of two alphoid repeat probes detec¬ting the centromeres of chromosomes 13 and 21 or chromosomes 14 and 22 respectively.

S. Kølvraa, J. Koch, N. Gregersen, P.K.A. Jensen, A.L. Jørgensen, K.B. Petersen, K. Rasmussen & L. Bolund.

Clinical Genetics 39 (1991) 278-286.


80.Genetisk forskning, fosterdiagnostik og behandling.

L. Bolund.

In Etiske spørgsmål i sundhedsvæsenet.

Amtsrådsforeningen i Danmark (1991) 53-57.


81.Morphological differentiation and changes in polypeptide synthesis pattern during regeneration of human epidermal tissue developed in vitro.

P.K.A. Jensen, S. Fey, P. Mose Larsen, J.O.R. Nørgård & L. Bolund.

Differentiation 47 (1991) 37-48.


82.DNA deletions in the low density lipoprotein (LDL) receptor gene in Danish families with familial hypercholesterolemia.

N. Rüdiger, E-M. Heinsvig, F.A. Hansen, O. Færgeman,

L. Bolund & N. Gregersen.

Clinical Genetics 39 (1991) 451-462.


83.Repetitive sequences involved in the recombination leading to deletion of exon 5 of the low-density-lipoprotein receptor gene in a patient with familial hyperchole¬sterolemia.

N. Rüdiger, P. Hansen, M. Jørgensen, O. Færgeman, L. Bolund & N. Gregersen.

Eur. J. Biochem. 198 (1991) 107-111.


84.In situ labelling of nucleic acids for gene mapping, diagnostics and functional cytogenetics.

J. Hindkjær, J. Koch, J. Mogensen, S. Pedersen, H. Fischer, M. Nygård, S. Junker, N. Gregersen, S. Kølvraa & L. Bolund.

In Advances in Molecular Genetics 4. Eds. J. Collins & A.J. Driesel. Hüthig (1991)

45-59. (Published in a modified version as 93).


85.The most common mutation causing medium-chain acyl - CoA dehydrogenase deficiency is strongly associated with a particular haplotype in the region of the gene.

S. Kølvraa, N. Gregersen, A. Blakemore, A.K. Schneidermann, V. Winter, B. Andresen, D. Curtis, P. Engel & L. Bolund.

Human Genetics 87 (1991) 425-428.


86.Characterization of a disease-causing Lys329 to Glu mutation in 16 patients with medium-chain acyl-CoA dehydrogenase deficiency.

N. Gregersen, B.S. Andresen, P. Bross, V. Winter, N. Rüdiger, S. Engst, S. Ghisla, E. Christensen, D. Kelly, A.W. Strauss, S. Kølvraa, L. Bolund, A. Blakemore, D. Curtis & P. Engel.

J. Inher. Metab. Dis. 14 (1991) 314-316.


87.An improved method for chromosome-specific labelling of alpha satellite DNA in situ by using denatured double stranded DNA probes as primers in a PRimed IN Situ labelling (PRINS) procedure.

J. Koch, J. Hindkjær, J. Mogensen, S. Kølvraa & L. Bolund.

Genetic Analysis (GATA) 8; 6 (1991) 171-178.


88.Non-radioactive, sequence-specific detection of RNA in situ by PRimed IN Situ labelling (PRINS).

J. Mogensen, S. Kølvraa, J. Hindkjær, S. Pedersen, J. Koch, M. Nygård, T. Jensen, N. Gregersen, S. Junker & L. Bolund.

Exptl. Cell Res. 196 (1991) 92-98.


89.Tissue culture of human epidermal keratinocytes: A differen¬tiating model system for gene testing and somatic gene therapy.

P.K.A. Jensen & L. Bolund.

J. Cell Science 100 (1991) 255-259.


90.EG-samarbetet inom den medicinska genetiken: Nya möjligheter för nordiska forskare.

L. Bolund.

Finska Läkaresällskapets Handlingar 135; 4 (1991) 335-337.

(Published in a modified version as 91).


91.The EC Human Genome Analysis Programme: Opportunities for Nordic human genetics and molecular biology.

L. Bolund.

NOMBA bulletin 2 (1991) 9.


92. Specific diagnosis of medium-chain acyl-CoA dehydrogenase (MCAD) deficiency in dried blood spots by a polymerase chain reaction (PCR) assay detecting a point-mutation (G985) in the MCAD gene.

N. Gregersen, A.I.F. Blakemore, V. Winter, B. Andresen, S. Kølvraa, L. Bolund, D. Curtis & P.C. Engel.

Clin. Chem. Acta 203 (1991) 23-34.


93.Primed in situ labelling of nucleic acids.

J. Hindkjær, J. Koch, J. Mogensen, S. Pedersen, H. Fischer, M. Nygaard, S. Junker, N. Gregersen, S. Kølvraa, A.J. Therkelsen & L. Bolund.

Biotech Forum Europe 8; 12 (1991) 752-756.


94.Fast one-step procedure for the detection of nucleic acids in situ by primer-induced sequence-specific labeling with fluorescein-12-dUTP.

J. Koch, J. Mogensen, S. Pedersen, H. Fischer, J. Hindkjær, S. Kølvraa & L. Bolund.

Cytogenetics and Cell Genetics 60 (1992) 1-3.

(Published in a modified version as 98).


95.Mellem-kædet acyl-CoA dehydrogenase (MCAD)-mangel: En livstruende fedtsyreoxi-dationsdefekt.

N. Gregersen, V. Winter, B. Andresen, S. Kølvraa, E. Christensen, B. Nørgaard Petersen & L. Bolund.

Ugeskrift for læger 154; 8 (1992) 483-488.


96.Alpha_1_antitrypsin alleles in patients with pulmonary emphysema detected by DNA amplification (PCR) and oligonucleotide probes.

K.B. Petersen, G.B. Petersen, R. Dahl, B. Larsen, S. Kølvraa, J. Koch, L. Bolund & N. Gregersen.

Eur. Respir. J. 5 (1992) 531-537.


97.Assignment of the gene for urokinase-type plasminogen activator receptor gene (PLAUR) to chromosome 19q13.1-q13.2.

A.D. Børglum, A. Byskov, P. Ragno, A.L. Roldan, P. Tripputi, G. Cassani, K. Danø, F. Blasi, L. Bolund & T.A. Kruse.

American J. Human Genetics 50 (1992) 492-497.


98.Fast one-step procedure for the detection of chromosomal DNA in situ by primer-induced sequence-specific labelling with fluorescein-12-dUTP.

J. Koch, J. Mogensen, S. Pedersen, H. Fischer, J. Hindkjær, S. Kølvraa & L. Bolund.

Colloquium Boehringer Mannheim Biochemica 2 (1992) 3-4.


99.Expression of wild-type and mutant medium-chain acyl-CoA dehydrogenase (MCAD) cDNA in eucaryotic cells.

T.G. Jensen, B.S. Andresen, P. Bross, U.B. Jensen, I. Holme, S. Kølvraa, N. Gregersen & L. Bolund.

Biochim. Biophys. Acta 1180 (1992) 65-72.


100.Characterization of medium-chain acyl-CoA dehydrogenase (MCAD) with a point mutation associated with MCAD deficiency.

P. Bross, T. Jensen, F. Kräutle, V. Winter, B.S. Andresen, S. Ernst, L. Bolund, S. Kølvraa, S. Ghisla, I. Rasched & N. Gregersen.

In New development in fatty acid oxidation. Eds. Coates P.M. & Tanaka K.

Wiley-Liss, New York (1992) 473-478.


101.Lateral growth and terminal differentiation during repeated epidermal regeneration in vitro: Age dependence and modulation by cholera toxin.

P.K.A. Jensen, J.O.R. Nørgård & L. Bolund.

Virchows Arch. B. Cell Path. 62 (1992) 189-198.


102.Molecular analysis of medium-chain acyl-CoA dehydrogenase deficiency: A diagnostic approach.

N. Gregersen, V. Winter, S. Kølvraa, B. Andresen, P. Bross, A. Blakemore, D. Curtis & L. Bolund.

In New development in fatty acid oxidation. Eds. Coates P.M. & Tanaka K.

Wiley-Liss, New York (1992) 441-452.


103.First report of the EC Working Group on Human Embryos and Research

L. Bolund (chairman).

Commission of the European Communities DG XII/E-4 (1992).


104.Determination of the frequency of medium-chain acyl-CoA dehydrogenase (MCAD) deficiency in England and Denmark by a PCR/restriction enzyme cleavage method.

N. Gregersen, B.N. Petersen, V. Winter, I. Knudsen, K. Schneidermann, S. Kølvraa, L. Bolund, A. Blakemore & D. Curtis.

In Proceedings from the 8th International Neonatal Screening Symposium.

The Kelvin Press, New South Wales, Australia

(1993) 232-233.


105.Genetic analysis of repeated, biparental, diploid, hydatidiform moles.

L. Sunde, L.O. Vejerslev, M.P. Jensen, S. Pedersen, J.M. Hertz & L. Bolund.

Cancer Genet. Cytogenet. 66 (1993) 16-22.


106.Repeated PRimed IN Situ labeling: formation and labeling of specific DNA sequences in chromosomes and nuclei.

C. Terkelsen, J. Koch, S. Kølvraa, J. Hindkjær, S. Pedersen & L. Bolund.

Cytogenetics and Cell Genetics 63 (1993) 235-237.

(Published in a modified version as 116).


107.Identification of a supernumerary der(18) chromosome by a rational strategy for the cytogenetic typing of small marker chromosomes with chromosome-specific DNA probes.

J. Koch, H. Fischer, H. Askholm, J. Hindkjær, S. Pedersen, S. Kølvraa & L. Bolund.

Clinical Genetics 43 (1993) 200-203.


108.A silent A to G mutation in exon 11 of the medium-chain acyl-CoA dehydrogenase (MCAD) gene.

B.S. Andresen, S. Kølvraa, P. Bross, L. Bolund, D. Curtis, H. Eiberg, Z. Zhang, D.P. Kelly, A.W. Strauss & N. Gregersen.

Human Molecular Genetics 2 (1993) 488.


109.What is Human Genome Analysis? The scientific foundations.

L. Bolund.

In Ethik in den Wissenschaften, Vol X. Ethics of Human Genome Analysis. European Perspectives. Eds. H. Haker, R. Hearn & K. Steigleder.

Attempto Verlag, Tübingen (1993) 15-18.


110.Assignment of an autosomal sex reversal locus (SRA1) and campomelic dysplasia (CMPD1) to 17q24.3-q25.1.

N. Tommerup, W. Schempp, P. Meinecke, S. Pedersen, L. Bolund, C. Brandt, C. Goodpasture, P. Guldberg, K.R. Held, H. Reinwein, O.D. Saugstad, G. Scherer, O. Skjeldal, R. Toder, J. Westvik, C.B. van der Hagen & U. Wolf.

Nature Genetics 4 (1993) 170.


111.Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency:

The prevalent mutation G985 (K304E) is subject to a strong founder effect from Northwestern Europe.

N. Gregersen, V. Winter, D. Curtis, T. Deufel, M. Mack, J. Hendrickx, P.J. Willems, A. Ponzone, T. Parrella, R. Ponzone, Jia-Huan Ding, Wen Zhang, Yuan Tsang Chen, S. Kahler, C.R. Roe, S. Kølvraa, K. Schneiderman. B.S. Andresen, P. Bross & L. Bolund.

Human Heredity 43 (1993) 342-350.


112.A rare disease-associated mutation in the medium-chain acyl-CoA dehydrogenase (MCAD) gene changes a conserved arginine, previously shown to be functionally essential in short-chain acyl-CoA dehydrogenase (SCAD).

B.S. Andresen, T.G. Jensen, P. Bross, I. Knudsen, V. Winter, S. Kølvraa, U.B. Jensen, L. Bolund, M. Duran, Jung-Ja Park Kim, D. Curtis, P. Divry, C. Vianey-Saban & N. Gregersen.

American J. Human Genetics 53 (1993) 730-739.


113.Co-overexpression of bacterial GroESL chaperonins partly overcomes non-productive folding and tetramer assembly of E. Coli-expressed human medium-chain acyl-CoA dehydrogenase (MCAD) carrying the prevalent disease-causing K304E mutation.

P. Bross, B.S. Andresen, V. Winter, F. Kräutle, T.G. Jensen, A. Nandy, S. Kølvraa, S. Ghisla, L. Bolund & N. Gregersen.

Biochim. Biophys. Acta 1182 (1993) 264-274.


114.Correction of steroid sulfatase deficiency by gene transfer into basal cells of tissue-cultured epidermis from patients with recessive X-linked ichthyosis.

T. Jensen, U.B. Jensen, P.K.A. Jensen, H.H. Ibsen, F. Brandrup, A. Ballabio & L. Bolund.

Exptl. Cell Res. 209 (1993) 392-397.


115.Sex dependent transmission of Beckwith-Wiedemann syndrome associated with a reciprocal translocation t(9;11)(p11.2; p15.5).

N. Tommerup, C.A. Brandt, S. Pedersen, L. Bolund & J. Kamper.

J. Med. Genet. 30 (1993) 958-961.


116.Repeated primed in situ labelling of specific DNA sequences ("PCR-PRINS") in metaphase chromosomal spreads.

C. Terkelsen, J. Koch, S. Kølvraa, J. Hindkjær, S. Pedersen & L. Bolund.

Colloquium Boehringer Mannheim Biochemica 3 (1993) 10-12.


117.A new, highly informative SmaI polymorphism in intron 7 of the low density lipoprotein receptor (LDLR) gene.

L.G. Jensen, H.K. Jensen, M. Kjeldsen, L.U. Gerdes, P.S. Hansen, O. Færgeman, S. Kølvraa, L. Bolund & N. Gregersen.

Clinical Genetics 45 (1994) 52-53.


118.Disorders of mitochondrial fatty acid oxidation - especially medium-chain acyl-CoA dehydrogenase (MCAD) deficiency.

N. Gregersen, B.S. Andresen, P. Bross, L. Bolund & S. Kølvraa.

In New Horizons in Neonatal Screening. Eds. J.-P. Farriaux & J.-L. Dhondt.

Elsevier Science B.V. (1994) 247-255.


119.A rare silent C to T mutation in exon 7 of the low density lipoprotein receptor (LDL-R) gene.

H.K. Jensen, L.G. Jensen, P.S. Hansen, M. Kjeldsen, L. Bolund, O. Færgeman & N. Gregersen.

Clinical Genetics 45 (1994) 54-55.


120.Value of chromosome painting in determining the chromosomal outcome in offspring of a 12;16 translocation carrier.

C.A. Brandt, T. Lyngbye, S. Pedersen, L. Bolund & U. Friedrich.

J. Med. Genet. 31 (1994) 234-237.


121.Fast, sensitive multicolor detection of nucleic acids in situ by PRimed IN Situ labeling (PRINS).

J. Hindkjær, J. Koch, C. Terkelsen, C.A. Brandt, S. Kølvraa & L. Bolund.

Cytogenet. Cell Genet. 66 (1994) 152-154.


122.Molecular genetic characterization and urinary excretion pattern of metabolites in two families with MCAD deficiency due to compound heterozygocity with a 13 basepair insertion in one allele.

N. Gregersen, V. Winter, S. Lyonnet, J.-M. Saudubray, U. Wendel, T.G. Jensen, B.S. Andresen, S. Kølvraa, W. Lehnert, L. Bolund, E. Christensen & P. Bross.

J. Inher. Metab. Dis. 17 (1994) 169-184.


123.Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency due to heterozygocity for the common mutation and an allele resulting in low levels of MCAD mRNA.

B.S. Andresen, P. Bross, I. Knudsen, V. Winter, S. Kølvraa, L. Bolund & N. Gregersen.

J. Inher. Metab. Dis. 17 (1994) 275-278.


124.Disease-causing mutations in exon 11 of the medium-chain acyl-CoA dehydrogenase gene.

B.S. Andresen, T.G. Jensen, P. Bross, I. Knudsen, V. Winter, S. Kølvraa, L. Bolund, Jia-Huan Ding, Yuan-Tsong Chen, J.L.K. VanHove, D. Curtis, I. Yokota, K. Tanaka, Jung-Ja Park Kim & N. Gregersen.

American J. Human Genetics 54 (1994) 975-988.


125.Reverse Chromosome Painting.

S. Pedersen, J. Hindkjær, C.A. Brandt, L. Bolund & S. Kølvraa.

In Methods in Molecular Biology, Vol 10. In situ hybridization protocols, Chapter 3. Ed. K.H.A. Choo.

Cliffon, New Jersey: Humana Press 33 (1994) 23-33.


126.PRimed IN Situ (PRINS) labeling of DNA.

J. Hindkjær, J. Koch, J. Mogensen, S. Kølvraa & L. Bolund.

In Methods in Molecular Biology, Vol 10. In situ hybridization protocols, Chapter 9. Ed. K.H.A. Choo.

Cliffon, New Jersey: Humana Press 33 (1994) 95-107.


127.PRimed IN Situ (PRINS) labeling of RNA.

J. Mogensen, S. Pedersen, J. Hindkjær, S. Kølvraa & L. Bolund.

In Methods in Molecular Biology, Vol 10. In situ hybridization protocols, Chapter 23. Ed. K.H.A. Choo.

Cliffon, New Jersey: Humana Press 33 (1994) 265-275.


128.Characterization of wild-type human medium-chain acyl-CoA dehydrogenase (MCAD) and mutant enzymes present in MCAD-deficient patients by two-dimensional gel electrophoresis: Evidence for post-translational modification of the enzyme.

P. Bross, T.G. Jensen, B.S. Andresen, M. Kjeldsen, A. Nandy, S. Kølvraa, S. Ghisla, I. Rasched, L. Bolund & N. Gregersen.

Biochem. Med. and Metabol. Biol. 52 (1994) 36-44.


129.Medicinsk Genetik.

L. Bolund.

Medicinsk Kompendium, 14th edition, Chapter 12.

Nyt Nordisk Forlag Arnold Busck A/S (1994) 127-138.


130.Characterization of a disease-causing Glu119-Lys mutation in the low density lipoprotein receptor gene in two Danish families with heterozygous familial hypercholesterolemia.

H.K. Jensen, T.G. Jensen, L.G. Jensen, P.S. Hansen, M. Kjeldsen, B.S. Andresen, V. Nielsen, H. Meinertz, A-B. B. Hansen, L. Bolund, O. Færgeman & N. Gregersen.

Human Mutation 4 (1994) 102-113.


131.Gene transfer into cultured human epidermis and its transplantation onto immunodefici¬ent mice: An experimental model for somatic gene therapy.

U.B. Jensen, T.G. Jensen, P.K.A. Jensen, J. Rygaard, B. Sehested Hansen, J. Fogh, S. Kølvraa & L. Bolund.

J. Invest. Dermatol. 103 (1994) 391-394.


132.An alanine29-serine variant in exon 2 of the low density lipoprotein gene: No association with hypercholesterolemia.

H.K. Jensen, L.G. Jensen, P.S. Hansen, L.S. Petersen, L.U. Gerdes, L. Bolund, O. Færgeman & N. Gregersen.

Clinical Genetics 46 (1994) 214-215.


133.Simultaneous detection of centromere specific probes and chromosome painting libraries by a combination of PRimed IN Situ labeling and chromosome painting (PRINS-painting).

J. Hindkjær, C.A. Brandt, J. Koch, T.B. Lund, S. Kølvraa & L. Bolund.

Chromosome Research 3 (1995) 41-44.


134.Complexity of molecular genetics of dyslipidemia in a family highly susceptible for ischemic heart disease.

H.K. Jensen, P.S Hansen, L.G. Jensen, M.J. Kristensen, I.C. Klausen, M. Kjeldsen, L. Lemming, L. Bolund, N. Gregersen & O. Færgeman.

Clinical Genetics 48 (1995) 23-28.


135.Molecular diagnosis and characterization of medium-chain acyl-CoA dehydrogenase deficiency.

B.S. Andresen, P. Bross, T.G. Jensen, I. Knudsen, V. Winter, S. Kølvraa, L. Bolund & N. Gregersen.

Scand. J. Clin. Lab. Invest. 55, Suppl. 220 (1995) 9-26.


136.Comparison between Medium-Chain Acyl-CoA Dehydrogenase mutant proteins over-expressed in bacterial and mammalian cells.

T.G. Jensen, P. Bross, B.S. Andresen, T.B. Lund, T.J. Kristensen, U.B. Jensen, V. Winther, S. Kølvraa, N. Gregersen & L. Bolund.

Human Mutation 6 (1995) 226-231.


137.Effects of two mutations detected in medium-chain acyl-CoA dehydrogenase (MCAD) deficient patients on folding, oligomer assembly and stability of MCAD enzyme.

P. Bross, C. Jespersen, T.G. Jensen, B.S. Andresen, M.J. Kristensen, V. Winter, A. Nandy, F. Kräutle, S. Ghisla, L. Bolund, Jung-Ja Park Kim and N. Gregersen.

J. Biol. Chem. 270 (1995) 10284-10290.


138.Construction of a panel of chromosome specific oligonucleotide probes (PRINS primers) useful for the identification of individual human chromosomes in situ.

J. Koch, J. Hindkjær, S. Kølvraa & L. Bolund.

Cytogenet. Cell Genet. 71 (1995) 142-147.


139.Hvor står genterapien i dag?

L. Bolund.

In Genterapi. Proceedings Christiansborg (1995) 6-8.


140.Medicinsk genetik og genterapi.

L. Bolund.

In Teknologinævnets rapport 1995/6 (1995) 60-64.


141.Biomedicinsk baggrund: Hvad ved vi?

L. Bolund.

In Genterapi på människa - Vad kan vi? Vad bör vi?

Nordiska Ministerrådet. Nord 1996:9 (1996) 7-10.


142.Ethylmalonic aciduria is associated with an amino acid variant of short chain acyl-CoA dehydrogenase.

M.J. Corydon, N. Gregersen, W. Lehnert, A. Ribes, P. Rinaldo, S. Kmoch, E. Christensen, T.J. Kristensen, B.S. Andresen, P. Bross, V. Winter, G. Martinez, S. Neve, T.G. Jensen, L. Bolund & S. Kølvraa.

Pediatric Research. 39 (1996) 1059-1066.

143.An LDL receptor promoter mutation in a heterozygous FH patient with dramatically skewed ratio between the two allelic mRNA variants.

L.G. Jensen, H.K. Jensen, H. Nissen. K. Kristiansen, O. Færgeman, L. Bolund & N. Gregersen.

Human Mutation 7 (1996) 82-84.


144.Rapid characterization of disease-causing mutations in the low density lipoprotein receptor (LDL-R) gene by over-expression in COS cells.

T.G. Jensen, B.S. Andresen, H.K. Jensen, L.G. Jensen, F. Heath, S. Pedesen, V. Nielsen, U.B. Jensen, T.B. Lund, N. Gregersen, S. Kølvraa & L. Bolund.

German J. Gastroenterology. 34, Suppl. 3 (1996) 9-12.


145.A flow cytometric competition technique for measuring interaction of LDL with cellular LDL-receptors applied to patients with mutant (Arg3500?Gln) apolipoprotein B.

F. Heath, T.G. Jensen, P.S. Hansen, L.G. Jensen, H. Vissinger, S.K. Moestrup, S. Pedersen, S. Kølvraa, L. Bolund & O. Færgeman.

German J. Gastroenterology. 34, Suppl. 3 (1996) 21-24.


146.A G-1-to-A acceptor splice site LDLR mutant allele leads to reduced relative transcript levels in patients with heterozygous familial hypercholesterolemia.

H.K. Jensen, L.G. Jensen, P.S. Hansen, L. Bolund, O. Færgeman & N. Gregersen.

Clinical Genetics 49 (1996) 175-179.


147.A signal of telomeric sequences on por¬cine chromosome 6q21-q22 detected by primed in situ labelling.

Feng Gu, J. Hindkjær, I. Gustavsson & L. Bolund.

Chromosome Research. 4 (1996) 251-252.


148.Allele specific measurement of low-density lipoprotein receptor transcript levels.

L.G. Jensen, H.K. Jensen, F. Heath, H. Eiberg, M. Kjeldsen, O. Færgeman, S. Kølvraa, L. Bolund & N. Gregersen.

Human Mutation. 8 (1996) 126-133.


149.Detection of nucleic acids (DNA and RNA) in situ by single and cyclic PRimed IN Situ labelling (PRINS): Two alternatives to traditional in situ hybridization methods.

J. Hindkjær, C. Terkelsen, S. Kølvraa, J. Koch & L. Bolund.

In Molecular Biotechnology. In Situ Hybridization, Chapter 3. Ed. M. Clark.

Chapman & Hall, Weinheim (1996) 45-66.


150.Flow cytometric DNA analyses of 105 fresh hydatidiform moles - with correlations to prognosis.

L. Sunde, B. Mogensen, S. Olsen, V. Nielsen, I.J. Christensen & L. Bolund.

Anlyt. Cell. Pathol. 12 (1996) 99-114.


151.Influence of Lewis alpha1-3/4-L-Fucosyltransferase (FUT3) gene mutations on enzyme activity, erythrocyte phenotyping and circulating tumor marker sialyl-Lewis a levels.

T.F. Ørntoft, E.M. Vestergaard, E. Holmes, J. Sinkbæk Jakobsen, N. Grunnet, M. Mortensen, P. Johnson, P. Bross, N. Gregersen, K. Skorstengaard, U.B. Jensen, L. Bolund & H. Wolf.

J. Biol. Chem. 271 (1996) 32260-32268.


152.Phenotypic characterization of a patient homozygous for the D558N LDL receptor gene mutation.

H.K. Jensen, L.G. Jensen, F. Heath, F. Melsen, P.S. Hansen, H. Meinertz, L. Bolund, N. Gregersen & O. Færgeman.

Clinical Genetics 50 (1996) 388-392.


153.PRimed IN Situ labelling (PRINS) as a rational procedure for identification of marker chromosomes using a panel of primers differentially tagging the human chromosomes.

J. Hindkjær, C.A. Brandt, H. Strømkjær, J. Koch, S. Kølvraa & L, Bolund.

Clinical Genetics 50 (1996) 437-441.


154.Primed In Situ Labeling (PRINS): A fast method for in situ labeling of nucleic acids.

J. Hindkjær, J. Koch, C. Brandt, S. Kølvraa & L. Bolund.

In Molecular Biotechnology, Vol. 6. (1996) 201-211.


155.Two mutations in the same low density lipoprotein receptor allele act in synergy to reduce receptor function in heterozygous familial hypercholesterolemia.

H.K. Jensen, T.G. Jensen, O. Færgeman, L.G. Jensen, B.S. Andresen, M.J. Corydon, P.H. Andreasen, P.S. Hansen, F. Heath, L. Bolund & N. Gregersen.

Human Mutation 9 (1997) 437-444.


156.A de novo complex t(7;13;8) with a deletion in the TRPS gene region.

C.A. Brandt, H-J Lüdecke, J. Hindkjær, H. Strømkjær, D. Pinkel, T. Herlin, L. Bolund & U. Friedrich.

Human Genetics 100 (1997) 334-338.


157.Den aktuelle kloningsforskning fra en sundhedsvidenskabelig synsvinkel.

L. Bolund.

In Høring om klonede dyr: Folketinget 9. April 1997.

Teknologirådets rapporter 1997/2 (1997) 34-43.


158.The molecular basis of medium-chain acyl-CoA dehydrogenase (MCAD) deficiency in compound heterozygous patients: Is there correlation between genotype and phenotype?

B.S. Andresen, P. Bross, S. Udvari, J. Kirk, G. Gray, S. Kmoch, N. Chamoles, I. Knudsen, V. Winter, B. Wilcken, I, Yokota, K. Hart, S. Packman, J.P. Harpey, J.M. Saudubray, D.E. Hale, L. Bolund, S. Kølvraa & N. Gregersen.

Hum. Mol. Gen. 6 (1997) 695-707.


159.A common W556S mutation in the LDL receptor gene of Danish patients with familial hypercholesterolemia encodes a transport-defective protein.

H.K. Jensen, H. Holst, L.G. Jensen, M.M. Jørgensen, P.H. Andreasen, T.G. Jensen, B.S. Andresen, F. Heath, P.S. Hansen, S. Neve, K. Kristiansen, O. Færgeman, S. Kølvraa, L. Bolund & N. Gregersen.

Atherosclerosis 131 (1997) 67-72.


160.Molecular cytogenetic analysis of a nontumorigenic human breast epithelial cell line that eventually turns tumorigenic: Validation of an analytical approach combining karyotyping, comparative genomic hybridization, chromosome painting, and single locus fluorescence in situ hybridization.

K.V. Nielsen, E. Niebuhr, B. Ejlertsen, S. Holstebroe, M.W. Madsen, P. Briand, H.T. Mouridsen & L. Bolund.

Genes Chromosomes and Cancer 20 (1997) 30-37.


161.Primed IN Situ labelling (PRINS): A rapid technique for in situ visualization of centromeres and telomeres.

S. Kølvraa, J. Hindkjær, J. Koch & L. Bolund.

In Cell Biology: A laboratory Handbook. Ed. J.E. Celis. Academic Press, New York

(1998) 437-444.


162.Identification of four new mutations in the short-chain acyl-CoA dehydrogenase (SCAD) gene in two patients: one of the variant alleles, 511C?T, is present at an unexpectedly high frequency in the general population, as was the case for 625G?A, together conferring susceptibility to ethylmalonic aciduria.

N. Gregersen, V.S. Winter, M.J. Corydon, T.J. Corydon, P. Rinaldo, A. Ribes, G. Martinez, M.J. Bennett, C. Vianey-Saban, A. Bhala, D.E. Hale, W. Lehnert, S. Kmoch, M. Roig, E. Riudor, H. Eiberg, B.S. Andresen, P. Bross, L.A. Bolund & S. Kølvraa.

Hum. Mol. Gen. 7 (1998) 619-627.


163.A human homologue of Escherichia coli ClpP caseinolytic protease:

recombinant expression, intracellular processing, and subcellular localization.

T.J. Corydon, P. Bross, H.U. Holst, S. Neve, K. Kristiansen, N. Gregersen & L. Bolund.

Biochem. J. 331 (1998) 309-316.


164.Rapid degradation of short-chain acyl-CoA dehydrogenase variants with temperature- sensitive folding defects occurs after import into mitochondria.

T.J. Corydon, P. Bross, T.G. Jensen, M.J. Corydon, T.B. Lund, U.B. Jensen, Jung-Ja Park Kim, N. Gregersen & L. Bolund.

J. Biol. Chem. 273 (1998) 13065-13071.


165.Identification of novel and known mutations in the genes for keratin 5 and 14 in Danish patients with epidermolysis bullosa simplex: Correlation between genotype and phenotype.

C.B. Sørensen, A.-S. Ladekjær-Mikkelsen, B.S. Andresen, F. Brandrup, N.K. Veien, S.K. Buus, I. Anton-Lamprecht, T.A. Kruse, P.K.A. Jensen, H. Eiberg, L. Bolund & N. Gregersen.

J. Invest. Dermatol. 112 (1999) 184-190.

166.Comparison of three methods for the detection of microsatellites.

M. Christensen, L. Sunde, L. Bolund & T.F. Ørntoft.

Scan. J. Clin. & Lab. Invest. 159 (1999) 167-178.


167.Clinical and molecular evidence of abnormal processing and trafficking of the vasopressin pre-prohormone in a large kindred with familial neurohypophyseal diabetes insipidus due to a signal peptide mutation.

C. Siggaard, S. Rittig, T.J. Corydon, P.H. Andreasen, T.G. Jensen, B.S. Andresen, G.L. Robertson, N. Gregersen, L. Bolund & E.B. Pedersen.

J. Clin. Endocrin. & Metabol. 84 (1999) 2933-2941.


168.Clear correlation of genotype with disease phenotype in very-long-chain acyl-CoA dehydrogenase deficiency.

B.S. Andresen, S. Olpin, B.J. Poorthuis, H.R. Scholte, C. Vianey-Saban, R. Wanders, L. Ijlst, A. Morris, M. Pourfarzam, K. Bartlett, E.R. Baumgartner, J.B.C. deKlerk, L.D. Schrøder, T.J. Corydon, H. Lund, V. Winter, P. Bross, L. Bolund & N. Gregersen.

Am. J. Hum. Genet. 64 (1999) 479-494.


169.Klinisk genetik. Et nyt tværgående grundspeciale.

C. Lundsteen, L. Bolund, A.-M. Gerdes, H. Goldstein, S. Kølvraa, G. Bruun Petersen, F. Skovby, S.A. Sørensen & N. Tommerup.

Ugeskrift for Læger. Klaringsrapport Nr. 5 (1999) 1-9.


170.Localization of a human nucleoporin 155 gene (Nup155) to the 5p13 region and cloning of its cDNA.

X. Zhang, H. Yang, M.J. Corydon, X. Zhang, S. Pedersen, J.R. Korenberg, X-N Chen, J. Laporte, N. Gregersen, E. Niebuhr, G. Liu & L. Bolund.

Genomics. 57 (1999) 144-151.


171.Somatisk genterapi.

T.G. Jensen & L. Bolund.

In Medicinsk årbog 1999. Munksgaard, København (1999) 145-151.


172.Medicinsk genetik.

L. Bolund.

Medicinsk Kompendium, 15th edition, Chapter 14.

Nyt Nordisk Forlag Arnold Busck A/S (1999) 158-171.


173.Protein misfolding and degradation in genetic diseases.

P. Bross, T.J. Corydon, B.S. Andresen, Malene M. Jørgensen, L. Bolund & N. Gregersen.

Human Mutation. 14 (1999) 186-198.


174.DNA-based prenatal diagnosis for very-long-chain acyl-CoA dehydrogenase deficiency.

B.S. Andresen, S. Olpin, E.A. Kvittingen, P. Augoustides-Savvopoulou, D. Lindhout, D.J.J. Halley, C. Vianey-Saban, R.J.A. Wanders, L. Ijlst, L.D. Schrøder, L. Bolund & N. Gregersen.

J. Inher. Metab. Dis. 22 (1999) 281-285.

175.Normolipidemia and hypercholesterolemia in persons heterozygous for the same 1592+5G?A splice site mutation in the LDL receptor gene.

H.K. Jensen, L.G. Jensen, H.U. Holst, P.H. Andreasen, P.S. Hansen, M.L. Larsen, S. Kølvraa, L. Bolund, N. Gregersen & O. Færgeman.

Clinical Genetics 56 (1999) 378-388.


176.Kortlægningen af menneskets arvemasse med håb om sygdomsforebyggelse og behandling - eller forestillinger om det genetisk designede menneske.

L. Bolund.

In Det Menneskeskabte Menneske. Det Etiske Råd, (1999) 21-28.


177.De genteknologiske valg.

L. Bolund, G. Christiansen, H. Dahl, C. Emmeche, P. Kemp, L. Klüver, S. Molin, T. Hviid Nielsen, J. Rosted, P. Sandøe & P. Saugmann-Jensen.

Erhvervsministeriet ISBN 87-601-7129-4 (1999) 1-148.


178.Complete loss of wild type TP53 in a non-transformed human breast epithelial cell line is preceded by a phase during which a heterozygous TP53 mutant effectively outgrows the homozygous wild type cells.

R. Villadsen, K.V. Nielsen, L. Bolund & P. Briand.

Cancer Genetics and Cytogenetics 116 (2000) 28-34.


179.Gener og sygdom.

J.M. Hertz, P.K.A. Jensen & L. Bolund.

In Odontologisk årbog, 2000. Munksgaard, København (2000) 69-84.


180.Keratinsygdomme.

P.K.A. Jensen, C.B. Sørensen, B.S. Andresen, F. Brandrup, N.K. Veien, S.K. Buus, N. Gregersen & L. Bolund.

Ugeskrift for Læger 162 (2000) 1867-1872.


181.Epidermolysis Bullosa Simplex: Korrelation mellem genotype og fænotype i danske patienter.

C.B. Sørensen, A.-S. Ladekjær-Mikkelsen, B.S. Andresen, F. Brandrup, N.K. Veien, S.K. Buus, I. Anton-Lamprecht. T. Kruse, P.K.A. Jensen, H. Eiberg, L. Bolund & N. Gregersen.

Ugeskrift for Læger 162 (2000) 1873-1876.


182.Transgene expression in human epidermal keratinocytes: Cell cycle arrest of productively transfected cells.

U.B. Jensen, M.S. Petersen, T.B. Lund, T.G. Jensen & L. Bolund.

Experimental Dermatology 9 (2000) 298-310.


183.Strain-specific variations in the development of dendritic cells in murine bone-marrow cultures.

M.S. Petersen, H.E. Toldbod, S. Holz, M. Hokland, L. Bolund & R. Agger.

Scandinavian Journal of Immunology 51 (2000) 586-594.


184.Characterization of the mouse Clpp protease cDNA, gene and protein.

B.S. Andresen, T.J. Corydon, M. Wilsbech, P. Bross, L.D. Schrøder, T.F. Hindkjær, L. Bolund & N. Gregersen.

Mammalian Genome 11 (2000) 275-280.


185.Grp78 is involved in retention of mutant low-density lipoprotein receptor protein in the endoplasmic reticulum.

M.M. Jørgensen, O.N. Jensen, H.U. Holst, J.-J. Hansen, T.J. Corydon, P. Bross, L. Bolund & N. Gregersen.

Journal of Biol Chem. 275 (2000) 33861-868.


186.Characterization of murine dendritic cells derived from adherent blood mononuclear cells in vitro.

R. Agger, M.S. Petersen, H.E. Toldbod, S. Holz, F. Dagnæs-Hansen, L. Bolund & M. Hokland.

Scandinavian Journal of Immunology 52 (2000) 138-147.


187.Human and mouse mitochondrial orthologs of bacterial ClpX.

T.J. Corydon, M. Wilsbech, C. Jespersgaard, B.S. Andresen, A.D. Børglum, S.Pedersen, L. Bolund, N. Gregersen & P. Bross.

Mammalian Genome 11(2000) 899-905.


188.A strategy combining flow sorting and comparative genomic hybridization for studying genetic aberrations at different stages of colorectal tumorigenesis in ulcerative colitis.

O.P.F. Clausen, S.N. Andersen, H. Strømkjær, V. Nielsen, T.O. Rognum, L. Bolund & S. Kølvraa.

Cytometry 43 (2001) 46-54.


189.PRimed IN Situ labeling (PRINS).

J. Hindkjær, L. Bolund & S. Kølvraa.

Methods in Cell Biology 64 (2001) 55-68.


190.Initial sequencing and analysis of the human genome.

L. Bolund as part of the International Human Genome Sequencing Consortium.

Nature 409 (2001) 860-921.


191.Det humane genomprojekt - status og perspektiver.

A.D. Børglum & L. Bolund

Ugeskrift for Læger 163 (2001) 4853-6.


192.The role of chaperone- assisted folding and quality control in inborn errors of metabolism: protein folding disorders.

N. Gregersen, P. Bross, B.S. Andresen, C.B. Pedersen, T.J.Corydon and L. Bolund.

Journal of Inherited Metabolic Disease 24 (2001) 189-212.



193.Mutation analysis in mitochondrial fatty acid oxidation defects: Exemplified by acyl-CoA dehydrogenase deficiencies, with special focus on Genotype-Phenotype relationship.

N. Gregersen, B.S. Andresen, M.J. Corydon, T.J. Corydon, R.K.J. Olsen, L. Bolund & P. Bross.

Human Mutation 18 (2001) 169-189.


194.LDL receptor-GFP fusion proteins: new tools for the characterisation of disease-causing mutations in the LDL receptor gene.

H.U. Holst, F. Dagnaes-Hansen, T.J. Corydon, P.H. Andreasen, M.M. Jørgensen,

S. Kølvraa, L. Bolund & T.G. Jensen.

European Journal of Human Genetics 9 (2001) 815-822.


195.Modulation of keratinocyte gene expression and differentiation by PPAR-selective ligands and tetradecylthioacetic acid.

M. Westergaard, J. Henningsen, M.L. Svendsen, C. Johansen, U.B. Jensen, H.D. Schrøder, I. Kratchmarova, R.K. Berge, L. Iversen, L. Bolund, K. Kragballe &

K. Kristiansen.

Journal of Investigative Dermatology 116 (2001) 702-712.


196.Genomic organization,transcript variants and comparative analysis of the human nucleoporin 155 (NUP155) gene.

X. Zhang, H. Yang, J. Yu, C. Chen, G. Zhang, J. Bao, Y. Du, M. Kibukawa, Z. Li, J. Wang, S. Hu, W. Dong, J. Wang, N. Gregersen, E. Niebuhr & L. Bolund.

Gene 288 (2002) 9-18.


197.Rapid genotyping of human ERCC1 exon 4 polymorphism with fluorescence analysis using fluorophore-labelled hybridization probes and a LightCyclerTM.

J. Yin, M. Hedayati, L. Grossman, L. Bolund & B.A. Nexø.

Biochemical Genetics 40 (2002) 143-148.


198.Association of chromosome 19q13.2-3 haplotypes with basal cell carcinoma: Tentative delineation of an involved region using data for single nucleotide polymorphisms in two cohorts.

E. Rockenbauer, M.H. Bendixen, Z. Bukowy, J. Yin, N.R. Jacobsen, M. Hedayati, U. Vogel, L. Grossman, L. Bolund & B.A. Nexø.

Carcinogenesis 23 (2002) 1149-1153.


199.Multiple single nucleotide polymorphisms on human chromosome 19q13.2-3 associate with risk of basal cell carcinoma.

J. Yin, E. Rockenbauer, M.A. Hedayati, N.R. Jacobsen, U. Vogel, L. Grossman, L. Bolund & B.A. Nexø.

Cancer Emidemiology, Biomarkers and Prevention 11 (2002) 1449-1453.


200.Mechanisms underlying targeted gene correction using chimeric RNA/DNA and single-stranded DNA oligonucleotides.

M.S. Andersen, C.B. Sørensen, L. Bolund & T.G. Jensen.

Journal of Molecular Medicine 80 (2002) 770-781.


201.Keratoacanthomas frequently show chromosomal aberrations as assessed by comparative genomic hybridization (CGH).

O.P.F. Clausen, M. Beigi, L. Bolund, S. Kølvraa, P. Jensen Gjersvik, G. Mørk & P.M. DeAngelis.

Journal of Investigative Dermatology 119 (2002) 1367-1372.


202.Grisetid.

Grisens arvemasse er nu ved at blive kortlagt i et stort dansk-kinesisk samarbejde

L. Bolund, M. Fredholm & C. Bendixen.

Ugeskrift for Læger 164 (2002) 6050-6051.


203. Genomic structure of the human mitochondrial chaperonin genes: Hsp60 and Hsp10 are localised head to head on chromosome 2 separated by a bidirectional promoter.

J.J. Hansen, P. Bross, M. Westergaard, M.N. Nielsen, H. Eiberg, A.D. Børglum, J. Mogensen, K. Kristiansen, L. Bolund & N. Gregersen.

Human Genetics 112 (2003) 71-77.


204.Twelve single nucleotide polymorphisms on chromosome 19q13.2-3: Linkage disequilibria and associations with basal cell carcinoma in Danish psoriatic patients.

J. Yin, U. Vogel, L.U. Gerdes, M. Dybdahl, L. Bolund & B.A. Nexø.

Biochemical Genetics 41 (2003) 27-37.


205.Konformationssygdomme.

N. Gregersen, P. Bross & L. Bolund.

Ugeskrift for Læger 165 (2003) 801-805.


206.Potent influence of bovine serum proteins in experimental dendritic cell based vaccination protocols.

H.E. Toldbod, R. Agger, L. Bolund & M. Hokland.

Scandinavian Journal of Immunology 58 (2003) 43-50.


207.Epidermolysis bullosa simplex keratinocytes with extended lifespan established by ectopic expression of telomerase.

T.G. Jensen, C.B. Sørensen, U.B. Jensen & L. Bolund.

Experimental Dermatology 12 (2003) 71-77.


208.Platelet-derived growth factor-responsive epidermis formed from human keratinocytes transduced with PDGF receptor beta.

O.Rollman, U.B. Jensen, A. Östman, L. Bolund, & T.G. Jensen.

Journal of Investigative Dermatology 120 (2003) 742-749.


209.Expression of short-chain acyl-CoA dehydrogenase (SCAD) proteins in the liver of SCAD deficient mice after hydrodynamic gene transfer.

D.A. Holm, F. Dagnæs-Hansen, H. Simonsen, N. Gregersen, L. Bolund, T.G. Jensen & T.J. Corydon.

Molecular Genetics and Metabolism 78 (2003) 250-258.


210.Functional testing of keratin 14 mutant proteins associated with the three major subtypes of Epidermolysis Bullosa Simplex.

C.B. Sørensen, B.S. Andresen, U.B. Jensen, T.G. Jensen, P.K.A. Jensen, N. Gregersen & L. Bolund.

Experimental Dermatology 12 (2003) 472-479.


211.Production of retroviral vectors in primary human keratinocytes after DNA mediated gene transfer leads to prolonged gene expression.

T.G. Jensen, U.B. Jensen & L. Bolund.

Acta Dermatologica and Venereologica 83 (2003) 83-87.


212.Expression and localization of peroxisome proliferator-activated receptors and nuclear factor ?B activity in normal and lesional psoriatic skin.

M. Westergaard, J. Henningsen, C. Johansen, M.L. Svendsen, U.B. Jensen, H.D. Schrøder, L. Iversen, L. Bolund, K. Kragballe & K. Kristiansen.

Journal of Investigative Dermatology 121 (2003) 1104-1117.


213.A population threshold for functional polymorphisms.

G.K-S. Wong, Z. Yang, D.A. Passey, M. Kibukawa, M. Paddock, N. Liu, L. Bolund & J. Yu.

Genome Research 13 (2003) 1873-1879.


214.Protein misfolding, aggregation and degradation in disease.

N. Gregersen, L. Bolund & P. Bross.

Methods in Molecular Biology 232 (2003) 3-16.


215.Integrating proteomic and functional genomic technologies in discovery driven translational breast cancer research.

J.E. Celis, P. Gromov. I. Gromova, J.M.A. Moreira, T. Cabezón, N. Ambartsumian, M. Grigorian, E. Lukanidin, P. thor Straten, P. Guldberg, J. Bartkova, J. Bartek, J. Lukas, C. Lukas, A. Lykkesfeldt, M. Jäättelä, P. Roepstorff, L. Bolund, T. Ørntoft, N. Brünner, J. Overgaard, K. Sandelin, M. Blicher-Toft, H. Mouridsen & F.E. Rank.

Molecular and Cellular Proteomics 2 (2003) 369-377.


216.Misfolding, degradation and aggregation of variant proteins - the molecular pathogenesis of short chain acyl-CoA dehydrogenase (SCAD) deficiency.

C.B. Pedersen, P. Bross, V.S. Winter, T.J. Corydon, L. Bolund, K. Bartlett, J. Vockley & N. Gregersen.

Journal of Biological Chemistry 48 (2003) 47449-47458.


217.Impaired trafficking of mutated AVP prohormone in cells expressing rare disease genes causing autosomal dominant familial neurohypophyseal diabetes insipidus.

J.H. Christensen, C. Siggaard, T.J. Corydon, G.L. Robertson, N. Gregersen, L. Bolund & S. Rittig.

Clinical Endocrinology 60 (2004) 125-136.


218.Side population (sp) cells in human and mouse epidermis do not have stem cell characteristics.

C. Triel, M.E. Vestergaard, L. Bolund, T.G. Jensen & U.B. Jensen.

Experimental Cell Research 295 (2004) 79-90.


219.Short PNA molecular beacons for real time PCR allelic discrimination of single nucleotide polymorphisms.

K. Petersen, U. Vogel, E. Rockenbauer, K.V. Nielsen, S. Kølvraa, L. Bolund & B. Nexø.

Molecular and Cellular Probes 18 (2004) 117-122.


220.Finishing the euchromatic sequence of the human genome.

L. Bolund as part of the International Human Genome Sequencing Consortium.

Nature 431 (2004) 931-945


221.Production of Transgenic Porcine Blastocysts by Handmade Cloning.

P.M. Kragh, G. Vajta, T.J. Corydon, S. Purup, L. Bolund & H. Callesen.

Reproduction, Fertility and Development 16 (2004) 315-318.


222.Differential cellular handling of defective arginine vasopressin (AVP) prohormones in cells expressing mutations of the AVP gene associated with autosomal dominant and recessive familial neurohypophyseal diabetes insipidus.

J.H. Christensen, C. Siggaard, T.J. Corydon, G.L. Robertson, N. Gregersen, L. Bolund & S. Rittig.

Journal of Clinical Endocrinology and Metabolism 89(9) (2004) 4521-4531..


223.Site-specific strand bias in gene correction of two different ß-galactosidase mutations using single-stranded oligonucleotides.

C.B. Sørensen, A. Krogsdam, M.S. Andersen, K. Kristiansen, L. Bolund & T.G. Jensen.

Journal of Molecular Medicine 83 (2005) 39-49.


224.High resolution mapping of genotype-phenotype relationships in Cri du Chat Syndrome using array CGH.

Xiaoxiao Zhang, A. Snijders, Xiuqing Zhang, R. Segraves, A. Niebuhr, D. Albertson, H. Yang, J. Gray, E. Niebuhr L. Bolund & D. Pinkel.

American Journal of Human Genetics 76 (2005) 312-326.


225.SNP genotyping using microsphere-linked PNA and flow cytometric detection.

E. Rockenbauer, K. Petersen, U. Vogel, L. Bolund, S. Kølvraa, K.V. Nielsen & B.A. Nexø.

Cytometry Part A 64A (2005) 80-86.


226.Expression of three different mutations in the arginine vasopressin gene suggests genotype-phenotype correlation in familial neurohypophyseal diabetes insipidus kindreds.

C. Siggaard, J.H. Christensen, T.J. Corydon, S. Rittig, G.L. Robinson, N. Gregersen, L. Bolund & E.B. Pedersen.

Clinical Endocrinology 63 (2005) 207-216.


227.“Beijing Region” (3pter-D3S3397) of the Human Genome:

Complete sequence and analysis.

Lars Bolund as part of the Chinese Human Genome Sequencing Consortium.

Science in China Ser. C Life Sciences 48 No.4 (2005) 311-329 (in English)

Science in China Ser. C Life Sciences 35 No.4 (2005) 286-303 (in Chinese)


228.Pigs in sequence space: A 0.66X coverage pig genome survey based on shotgun sequencing.

R. Wernersson, M.H. Schierup, F.G. Jørgensen, J. Garodkin, F. Panitz, H-H. Stærfeldt, O.F. Christensen, T. Mailund, H. Hjortshøj, A. Klein, Jun Wang, B. Liu, S. Hu, W. Dong, W. Li, G.K-S. Wong, J. Yu, Jian Wang, C. Bendixen, M. Fredholm, S. Brunak, H. Yang & L. Bolund.

BMC Genomics 6 (2005) 70.


229.Efficient in vitro production of porcine blastocysts by handmade cloning with a combined electrical and chemical activation.

P.M. Kragh, Y. Du, T.J. Corydon, S. Purup, L. Bolund & G. Vajta.

Theriogenology 64 nr.7 (2005) 1536-1545.


230.Protein Misfolding, Aggregation, and Degradation in Disease.

N. Gregersen, L. Bolund & P.G. Bross.

Molecular Biotechnology 31 nr.2 (2005) 141-150.


231.High overall in vitro efficiency of porcine handmade cloning (HMC) combining partial zona digestion and oocyte trisection with sequential culture.

Y. Du, P.M. Kragh, X. Zhang, S. Purup, H. Yang, L. Bolund & G. Vajta.

Cloning and Stem Cells 7 nr.3 (2005) 199-205.


232.Identification of genetic aberrations on chromosome 22 outside the NF2 locus in schwannomatosis and neurofibromatosis type 2.

P.G. Buckley, K.K. Matripragada, T. Díaz de Ståhl, A. Piotrowski, C. Hansson, H. Kiss, D. Ventrie, I.T. Ernberg, M. Nordenskjöld, L. Bolund, M. Saino, G.A. Rouleau, M. Niimura, A.J. Wallace, D.G.R. Evans, G. Grigelionis, U. Menzel & J.P. Dumanski.

Human Mutation 26 nr.6 (2005) 540-549.


233.A microarray analysis of the rice transcriptome and its comparison to Arabidopsis.

L. Ma, C. Chen, X. Liu, Y. Jiao, N. Su, L. Li, X. Wang, M. Cao, N. Sun, X. Zhang, J. Bao, D. Sun, J. Li, S. Pedersen, L. Bolund, H. Zhao, L. Yuan, G.K.S. Wong, J. Wang, X.W. Deng & J. Wang.

Genome Research 15 (2005) 1274-1283.


234.Kortlægning af grisens arvemasse.

M. Fredholm, C. Bendixen & L. Bolund.

Naturens Verden 5 (2005) 2-5.


235.Identification of novel deletion breakpoints bordered by segmental duplications in the NF1 locus using high resolution array-CGH.

K.K. Matripragada, A-C. Thuresson, A. Piotrowski, T. Díaz de Ståhl, U. Menzel, G. Grigelionis, R.E. Ferner, S. Griffiths, L. Bolund, V. Mautner, M. Nordling, E. Legius, D. Vetrie, N. Dahl, L. Messiaen, M. Upadhyaya, C.E.G. Bruder & J.P. Dumanski.

Journal of Medical Genetics 43 (2006) 28-38.


236.TreeFam: a curated database of phylogenetic trees of animal gene families.

H. Li, A. Coghlan, J. Ruan, L. Coin, J. Hériché, L. Osmotherly, R. Li, T. Liu, Z. Zhang, L. Bolund, G. Wong, W. Zheng, P. Dehal, J. Wang & R. Durbin.

Nucleic Acids Research 34 (2006) D572-D580.


237.WEGO: a web tool for plotting GO annotations.

J. Ye, L. Fang, H. Zheng, Y. Zhang, J. Chen, Z. Zhang, J. Wang, S. Li, R. Li, L. Bolund & J. Wang.

Nucleic Acids Research 34 (2006) W293-W297.


238. The DNA sequence, annotation and analysis of human chromosome 3.

Lars Bolund as part of the Chinese Human Genome Sequencing Consortium.

Nature 440 (2006) 1194-1198.


239.Are keratoacanthomas (KAs) variants of squamous cell carcinomas (SCCs)?

A comparison of genetic aberrations by comparative genomic hybridization.

O.P.F. Clausen, H.C.D. Aass, M. Beigi, K. Purdie, C.M. Proby, V.L. Brown, M. Mattingsdal, F.Micci, S. Kølvraa, L. Bolund & P.M. DeAngelis.

Journal of Investigative Dermatology 126 (2006) 2308-2315.


240.Chemically assisted handmade enucleation of porcine oocytes.

J. Li, Y. Du, Y.H. Zhang, P.M. Kragh, S. Purup, L. Bolund, H. Yang, Q.X. Xue & G. Vajta.

Cloning and Stem Cells 8 nr.4 (2006) 241-250.


241.Single-nucleotide variations in the genes encoding the mitochondrial Hsp60/Hsp10 chaperonin system and their disease-causing potential.

P. Bross, Z. Li, J. Hansen, J.J. Hansen, M.N. Nielsen, T.J. Corydon, C. Georgopolous, D. Ang, J.B. Lundemose, K. Niezen-Koning, H. Eiberg, H. Yang, S. Kølvraa, L. Bolund & N. Gregersen.

Journal of Human Genetics 52 (2007) 56-65.

242.PigGIS: Pig Genomic Informatics System.

J. Ruan, Y. Guo, H. Li, Y. Hu, L. Bolund & J. Wang.

Nucleic Acids Research 35 (2007) D654-D657.


243.Snap: An Integrated SNP Annotation Platform.

S. Li, L. Ma, H. Li, S. Vang, Y. Hu, L. Bolund & J. Wang.

Nucleic Acids Research 35 (2007) D707-D710.


244.Simplified cryopreservation of porcine cloned blastocysts.

Y. Du, Y. Zhang, J. Li, P.M. Kragh, M. Kuwayama, S. Ieda, X. Zhang, M. Schmidt, I.B. Bøgh, S. Purup, A.M. Pedersen, K. Villemoes, H. Yang, L. Bolund & G. Vajta.

Cryobiology 54 (2007) 181-187.


245.Porcine transcriptome analysis based on 97 non-normalized cDNA libraries and assembly of 1,021,891 ESTs.

J. Gorodkin, S. Ciera, J. Hedegaard, M.J. Gilchrist, F. Panitz, C.B. Jørgensen, K. Scheibye-Knudsen, T. Arvin, S. Lumholdt, M. Sawera, T. Green, B.J. Nielsen, J.H. Havgaard, C. Rosenkilde, J. Wang, H. Li, R. Li, B. Liu, S. Hu, W. Dong, W. Li, J. Yu, J. Wang, H-H. Stærfeldt, R. Wernersson, L. B. Madsen, B. Thomsen, H. Hjortshøj, Z. Bujie, X. Wang, X. Wang, L. Bolund, S. Brunak, H. Yang, C. Bendixen & M. Fredholm.

Genome Biology 8 (4) (2007) R45. 1-16.


246.Genetics of Healthy Aging in Europe: The EU-Integrated Project GEHA (GEnetics of Healthy Aging).

C. Franceschi, V. Bezrukov, H. Blanché, L. Bolund, K. Christensen, G. De Benedictis, L. Deiana, E. Gonos, A. Hervonen, H. Yang, B. Jeune, T.B. Kirkwood, P. Kristensen, A. Leon, P.G. Pellicci, L. Peltonen, M. Poulain, I.M. Rea, J. Remacle, J.M. Robine, S. Schreiber, E. Sikora, P.E. Slagboom, L. Spazzafumo, M.A. Stazi, O. Toussaint & J.W. Vaupel.

Annals of the New York Academy of Sciences 1100 (2007) 21-45.


247.Handling of human short-chain acyl-CoA dehydrogenase (SCAD) variant proteins in transgenic mice.

P.M. Kragh, C.B. Pedersen, S.P. Schmidt, V.S. Winter, G. Vajta, N. Gregersen, L. Bolund & T.J. Corydon.

Molecular Genetics and Metabolism 91 (2007) 128-137.


248.Piglets born from handmade cloning, an innovative cloning method without micromanipulation.

Y. Du, P.M. Kragh, Y. Zhang, J. Li, M. Schmidt, I.B. Bøgh, X. Zhang, S. Purup, A.L. Jørgensen, A.M. Pedersen, K. Villemoes, H. Yang, L. Bolund & G. Vajta.

Theriogenology 68:8 (2007) 1104-1110.


249.Piglets born from vitrified cloned blastocysts produced with a simplified method of delipation and nuclear transfer.

Y. Du, J. Li, P.M. Kragh, Y. Zhang, M. Schmidt, I.B. Bøgh, X. Zhang, S. Purup, A.L. Jørgensen, A.M. Pedersen, K. Villemoes, H. Yang, L. Bolund & G. Vajta.

Cloning and stem cells 9:4 (2007) 469-476.


250.High hydrostatic pressure improved developmental competence of porcine oocytes for handmade cloning,

Y. Du, L. Lin, C. Pribenszky, M. Molnár, P.M. Kragh, J. Li, X. Zhang, H. Yang, A.M. Pedersen, K. Villemoes, L. Bolund & G. Vajta.

Reproduction, Fertility and Development 20:1 (2007) 94-95.


251.High hydrostatic pressure: a new way to improve in vitro developmental competence of porcine matured oocytes after vitrification.

Y. Du, Cs. Pribenszky, M. Molnár, X. Zhang, H. Yang, M. Kuwayama, A.M. Pedersen, K. Villemoes, L. Bolund & G. Vajta.

Reproduction 135:1 (2008) 13-17.


252.TreeFam: 2008 Update

J. Ruan, H. Li, Z. Chen, A. Coghlan, L.J.M. Coin, Y. Guo, J-K. Hériché, Y. Hu, K. Kristiansen, R. Li, Y. Liu, A. Moses, J. Qin, S. Vang, A.J. Vilella, A. Ureta-Vidal, L. Bolund, J. Wang & R. Durbin.

Nucleic Acids Research 36 (2008) D735-D740.


253.Linkage disequilibrium mapping of a breast cancer susceptibility locus near RAI/PPP1R13L/iASPP.

B.A. Nexø, U. Vogel, A. Olsen, M. Nyegaard, Z. Bukowy, E. Rockenbauer, X. Zhang, C. Koca, M. Mains, B. Hansen, A. Hedemand, A. Kjeldgaard, M.J. Laska, O. Raaschou-Nielsen, S. Cold, K. Overvad, A. Tjønneland, L. Bolund & A.D. Børglum.

BMC Medical Genetics 9:56 (2008) 1-12, doi: 10.1186/1471-2350-9-56.


254.The ACADS gene variation in 114 patients with short-chain acyl-CoA dehydrogenase (SCAD) deficiency is dominated by missense variations leading to protein misfolding at the cellular level.

C.B. Pedersen, S. Kølvraa, A. Kølvraa, V. Stenbroen, M. Kjeldsen, R. Ensenauer, I. Tein, D. Matern, P. Rinaldo, C. Vianey-Saban, A. Ribes, W. Lehnert, E. Christensen, T.J. Corydon, B.S. Andresen, S. Vang, L. Bolund, J. Vockley, P. Bross & N. Gregersen.

Human Genetics 124:1 (2008) 43-56.


255.Twin pregnancies with diploid hydatidiform mole and coexisting normal fetus may originate from one oocyte.

I. Niemann, L. Bolund & L. Sunde.

Human Reproduction 23:9 (2008) 2031-2035.


256.The diploid genome sequence of an Asian individual.

Jun Wang, Wei Wang, Ruiqiang Li, Yingrui Li, Geng Tian, L. Goodman, Wei Fan, Junqing Zhang, Jun Li, Juanbin Zhang, Yiran Guo, Binxiao Feng, Heng Li, Yao Lu, Xiaodong Fang, Huiqing Liang, Zhenglin Du, Dong Li, Yiqing Zhao, Yujie Hu, Zhenzhen Yang, Hancheng Zheng, I. Hellmann, M. Inouye, J. Pool, Xin Yi, Jing Zhao, Jinjie Duan, Yan Zhou, Junjie Qin, Lijia Ma, Guoqing Li, Zhentao Yang, Guojie Zhang, Bin Yang, Chang Yu, Fang Liang, Wenjie Li, Shaochuan Li, Dawei Li, Peixiang Ni, Jue Ruan, Qibin Li, Hongmei Zhu, Dongyuan Liu, Zhike Lu, Ning Li, Guangwu Guo, Jianguo Zhang, Jia Ye, Lin Fang, Qin Hao, Quan Chen, Yu Liang, Yeyang Su, A. san, Cuo Ping, Shuang Yang, Fang Chen, Li Li, Ke Zhou, Hongkun Zheng, Yuanyuan Ren, Ling Yang, Yang Gao, Guohua Yang, Zhuo Li, Xiaoli Feng, K. Kristiansen, G. Ka-Shu Wong, R. Nielsen, R. Durbin, L. Bolund, Xiuqing Zhang, Songgang Li, Huanming Yang, Jian Wang

Nature 456 (2008) 60-65, doi: 10.1038/nature07484


257.High hydrostatic pressure treatment of porcine oocytes before handmade cloning improves developmental competence and cryosurvival.

Y. Du, L. Lin, M. Schmidt, I.B. Bøgh, P.M. Kragh, C.B. Sørensen, J. Li, S. Purup, Cz. Pribennszky, M. Molnár, M. Kuwayama, X. Zhang, H. Yang, L. Bolund & G. Vajta.

Cloning and Stem Cells 10:3 (2008) 325-330.


258.High in vitro development after somatic cell nuclear transfer and trichostatin A treatment of reconstructed porcine embryos.

J. Li, O. Svarcova, K. Villemoes, P.M. Kragh, M. Schmidt, I.B. Bøgh,Y. Zhang, Y. Du, L. Lin, Q. Xue, S. Purup, L. Bolund, H. Yang, P. Maddox-Hyttel & G. Vajta.

Theriogenology 70:5 (2008) 800-808.


259.Omics-based profiling of carcinoma of the breast and matched regional lymph node metastasis.

J. Li, P. Gromov, I. Gromova, J.M. Moreira, V. Timmermans-Wielenga, F. Rank, K. Wang, S. Li, H. Li, C. Wiuf, H. Yang, X. Zhang, L. Bolund & J.C. Celis.

Proteomics 8:23-24 (2008) 5038-5052.


260.EAGLES report on HIV/AIDS research.

R. Walgate, J. Degett & Eagles Steering Committee on Health with L.Bolund.

New Biotechnology 25:1 (2008) 29-33.


261.Efficiency of two enucleation methods connected to handmade cloning to produce transgenic porcine embryos.

J. Li, K. Villemoes, Y. Zhang, Y. Du, P.M. Kragh, S. Purup, Q. Xue, A.M. Pedersen, A.L. Jørgensen, J. Jakobsen, L. Bolund, H. Yang & G. Vajta.

Reproduction in Domestic Animals 44:1 (2009) 122-127.


262.Comparison of efficiency of open pulled straw (OPS) and cryotop vitrification for crypreservation of in vitro matured pig oocytes.

Y. Liu, Y. Du, L. Lin, J. Li, P.M. Kragh, M. Kuwayama, L. Bolund, H. Yang & G. Vajta.

Cryo-letters 29, 4 (2009) 315-320.


263.Estimation of tumor heterogeneity using CGH array data.

K. Wang, J. Li, S. Li, L. Bolund & C. Wiuf.

BMC Bioinformatics 10, 1 (2009) 12, doi: 10.1186/1471-2105-10-12.


264.Osmotic stress induced by sodium chloride, sucrose or trehalose improves cryotolerance and developmental competence.

L. Lin, P.M. Kragh, S. Purup, M. Kuwayama, Y. Du, X. Zhang, H. Yang, L. Bolund, H. Callesen & G. Vajta.

Reproduction, Fertility and Development 21, 2 (2009) 338-344.


265. Elevated NaCl concentration improves cryotolerance and developmental competence of porcine oocytes.

L. Lin, Y. Du, Y. Liu, P.M. Kragh, J. Li, S. Purup, M. Kuwayama, X. Zhang, H. Yang, L. Bolund & G. Vajta.

Reproductive BioMedicine Online 18, 3 (2009) 360-366.


266.Haplotype frequencies in a sub-region of chromosome 19q13.3, related to risk and prognosis of cancer, differ dramatically between ethnic groups.

M.H. Schierup, T. Mailund, H. Li, J. Wang, A. Tjønneland, U. Vogel, L. Bolund & B. A. Nexø.

BMC Medical Genetics 10 (2009) 20, doi: 10.1 186/1471-2350-10-20.


267.An improved method for genome wide DNA methylation profiling correlated to transcription and genomic instability in two breast cancer cell lines.

J. Li, F. Gao, N. Li, S. Li, G. Yin, G. Tian, S. Jia, K. Wang, X. Zhang, H. Yang, A.L. Nielsen & L. Bolund.

BMC Genomics 10 (2009) 223, doi: 10.1186/1471-2164-10-223.


268.DNA copy number aberrations in breast cancer by array comparative genomic hybridization.

J. Li, K. Wang, S. Li, V. Timmermans-Wielenga, F. Rank, C. Wiuf, X. Zhang, H. Yang, & L. Bolund.

Genomics Proteomics & Bioinformatics 7, 1-2 (2009) 13-24.


269.Hemizygous minipigs produced by random gene insertion and handmade cloning express the Alzheimer's disease-causing dominant mutation APPsw.

P.M. Kragh, A.L. Nielsen, J. Li, Y. Du, L. Lin, M. Schmidt, I.B. Bøgh, I.E. Holm, J.E. Jakobsen, M.G. Johansen, S. Purup, L. Bolund, G. Vajta & A.L. Jørgensen.

Transgenic Research 18, 4 (2009) 545-58.


270.Familial colorectal cancer, can it be identified by microsatellite instability and chromosomal instability? - A case-control study.

L. Sunde, M.L. Bisgaard, H. Soll-Johanning, N.O. Jacobsen, L. Bolund, J. Skouv & E. Lynge.

Cancer Biomarkers 5, 4 (2009) 197-205.


271.The genome of the cucumber, Cucumis sativus L.

S. Huang, R. Li, Z. Zhang, L. Li, X. Gu, W. Fan, W.J. Lucas, X. Wang, B. Xie, P. Ni, Y. Ren, H. Zhu, J. Li, K. Lin, W. Jin, Z. Fei, G. Li, J. Staub, A. Kilian, E.A.G. van der Vossen, Y. Wu, J. Guo, J. He, Z. Jia, Y. Ren, G. Tian, Y. Lu, J. Ruan, W. Qian, M. Wang, Q. Huang, B. Li, Z. Xuan, J. Cao, Asan, Z. Wu, J. Zhang, Q. Cai, Y. Bai, B. Zhao, Y. Han, Y. Li, X. Li, S. Wang, Q. Shi, S. Liu, W.K. Cho, J. Kim, Y. Xu, K. Heller-Uszynska, H. Miao, Z. Cheng, S. Zhang, J. Wu, Y. Yang, H. Kang, M. Li, H. Liang, X. Ren, Z. Shi, M. Wen, M. Jian, H. Yang, G. Zhang, Z. Yang, R. Chen, S. Liu, J. Li, L. Ma, H. Liu, Y. Zhou, J. Zhao, X. Fang, G. Li, L. Fang, Y. Li, D. Liu, H. Zheng, Y. Zhang, N. Qin, Z. Li, G. Yang, S. Yang, L. Bolund, K. Kristiansen, H. Zheng, S. Li, X. Zhang, H. Yang, J. Wang, R. Sun, B. Zhang, S. Jiang, J. Wang, Y. Du & S. Li.

Nature Genetics 41, 12 (2009) 1275-1281.


272.The sequence and de novo assembly of the giant panda genome.

R. Li, W. Fan, G. Tian, H. Zhu, L. He, J. Cai, Q. Huang, Q. Cai, B. Li, Y. Bai, Z. Zhang, Y. Zhang, W. Wang, J. Li, F. Wei, H. Li, M. Jian, J. Li, Z. Zhang, R. Nielsen, D. Li, W. Gu, Z. Yang, Z. Xuan, O.A. Ryder, F.C. Leung, Y. Zhou, J. Cao, X. Sun, Y. Fu, X. Fang, X. Guo, B. Wang, R. Hou, F. Shen, B. Mu, P. Ni, R. Lin, W. Qian, G. Wang, C. Yu, W. Nie, J. Wang, Z. Wu, H. Liang, J. Min, Q. Wu, S. Cheng, J. Ruan, M. Wang, Z. Shi, M. Wen, B. Liu, X. Ren, H. Zheng, D. Dong, K. Cook, G. Shan, H. Zhang, C. Kosiol, X. Xie, Z. Lu, H. Zheng, Y. Li, C.C. Steiner, T.T. Lam, S. Lin, Q. Zhang, G. Li, J. Tian, T. Gong, H. Liu, D. Zhang, L. Fang, C. Ye, J. Zhang, W. Hu, A. Xu, Y. Ren, G. Zhang, M.W. Bruford, Q. Li, L. Ma, Y. Guo, N. An, Y. Hu, Q. Zheng, Y. Shi, Z. Li, Q. Liu, Y. Chen, J. Zhao, N. Qu, S. Zhao, F. Tian, X. Wang, H. Wang, L. Xu, X. Liu, T. Vinar, Y. Wang, T. Lam, S. Yiu, S. Liu, H. Zhang, D. Li, Y. Huang, X. Wang, G. Yang, Z. Jiang, J. Wang, N. Qin, L. Li, J. Li, L. Bolund, K. Kristiansen, G.K. Wong, M. Olson, X. Zhang, S. Li, H. Yang, J. Wang & J. Wang.

Nature 463, 7279 (2010) 311-317.


273.Building the sequence map of the human pan-genome.

R. Li, Y. Li, H. Zheng, R. Luo, H. Zhu, Q. Li, W. Qian, Y. Ren, G. Tian, J. Li, G. Zhou, X. Zhu, H. Wu, J. Qin, X. Jin, D. Li, H. Cao, X. Hu, H. Blanche, H. Cann, X. Zhang, S. Li, L. Bolund, K. Kristiansen, H. Yang, J. Wang, & J. Wang.

Nature Biotechnology 28, 1 (2010) 57-63.


274.High hydrostatic pressure treatment of porcine oocytes induces parthenogenetic activation.

L. Lin, C. Pribenszky, M. Molnár, P.M. Kragh, Y. Du, X. Zhang, H. Yang, L. Bolund, H. Callesen, Z. Macháty & G. Vajta.

Cellular reprogramming 12, 4 (2010) 475-480.


275.Pig genome sequence - analysis and publication strategy.

A.L. Archibald, L. Bolund, C. Churcher, M. Fredholm, M.A.M. Groenen, B. Harlizius, K.-T. Lee, D. Milan, J. Rogers, M.F. Rothschild, H. Uenishi, J. Wang & L.B. Schook; Genome Sequencing Consortium, Swine.

BMC Genomics 11, 1 (2010) 438.


276.Resequencing of 200 human exomes identifies an excess of low-frequency non-synonymous coding variants.

Y. Li, N. Vinckenbosch, G. Tian, E. Huerta-Sanchez, T. Jiang, H. Jiang, A. Albrechtsen, G. Andersen, H. Cao, T. Korneliussen, N. Grarup, Y. Guo, I. Hellman, X. Jin, Q. Li, J. Liu, X. Liu, T. Sparsø, M. Tang, H. Wu, R. Wu, C. Yu, H. Zheng, A. Astrup, L. Bolund, J. Holmkvist, T. Jørgensen, K. Kristiansen, O. Schmitz, T.W. Schwartz, X. Zhang, R. Li, H. Yang, J. Wang, T. Hansen, O. Pedersen, R. Nielsen & J. Wang.

Nature Genetics 42, 11 (2010) 969-972, doi: 10.1038/ng.680.


277.Sequencing bias: comparison of different protocols of microRNA library construction.

G. Tian, X. Yin, H. Luo, X. Xu, L. Bolund, X. Zhang, S. Gan & L.Ning.

BMC Biotechnology 10 (2010) 64, doi: 10.1186/1472-6750-10-64.


278.Sequencing of 50 human exomes reveals adaptation to high altitude.

X. Yi, Y. Liang, E. Huerta-Sanchez, X. Jin, Z.-X.-P. Cuo, J.E. Pool, X. Xu, H. Jiang, N. Vinckenbosch, T.S. Korneliussen, H. Zheng, T. Liu, W. He, K. Li, R. Luo, X. Nie, H. Wu, M. Zhao, H. Cao, J Zou, Y. Shan, S. Li, Q. Yang, Asan, P. Ni, G. Tian, J. Xu, X. Liu, T. Jiang, R. Wu, G. Zhou, M. Tang, J. Qin, T. Wang, S. Feng, G. Li, Huasang, J. Luosang, W. Wang, F. Chen, Y. Wang, X. Zheng, Z. Li, Z. Bianba, G. Yang, X. Wang, S. Tang, G. Gao, Y. Chen, Z. Luo, L. Gusang, Z. Cao, Q. Zhang, W. Ouyang, X. Ren, H. Liang, H Zheng, Y. Huang, J. Li, L. Bolund, K. Kristiansen, Y. Li, Y. Zhang, X. Zhang, R. Li, S. Li, H. Yang, R. Nielsen, J. Wang, & J. Wang.

Science (New York, N.Y.) 329, 5987 (2010) 75-78.


279.State of the art de novo assembly of human genomes from massively parallel sequencing data.

Y. Li, Y. Hu, L. Bolund & J. Wang.

Human Genomics 4, 4 (2010) 271-277.


280.Stress for Stress Tolerance? A Fundamentally New Approach in Mammalian Embryology.

C. Pribenszky, G. Vajta, M. Molnar, Y. Du, L. Lin, L. Bolund & J. Yovich.

Biology of Reproduction 83, 5 (2010) 690-697, doi:10.1095/biolreprod.110.083386.


281.Somatic Point Mutations in mtDNA Control Region Are Influenced by Genetic Background and Associated with Healthy Aging: A GEHA Study.

G. Rose, G. Romeo, S. Dato, P. Crocco, A.C. Bruni, A. Hervonen, K. Majamaa, F. Sevini, C. Franceschi, G. Passarino & the GEHA Project Consortium (including L. Bolund).

PLoS One 5 (2010) 10, doi: 10.1371/journal.pone.0013395.


282.Tumor heterogeneity in neoplasms of breast, colon, and skin.

J. Li, K. Wang, T.D.Jensen, S. Li, L. Bolund & C.H.Wiuf.

BMC Research Notes 3 (2010) 321.


283.TAO rests in its own Nature: The path of the Sage is called Following the Light of Nature.

L. Bolund.

In: China The Way Forward. Hong Kong (2010) 12-13 and 80-81.


284.The DNA methylome of human peripheral blood mononuclear cells.

Y. Li, J. Zhu, G. Tian, N. Li, Q. Li, M. Ye, H. Zheng, J. Yu, H. Wu, J. Sun, Z. Hongyu, Q. Chen, R. Luo, M. Chen, Y. He, X. Jin, Q. Zhang, C. Yu, G. Zhou, J. Sun, Y. Huang, H. Zheng, H. Cao, X. Zhou, S. Guo, X. Hu, X. Li, K. Kristiansen, L. Bolund, J. Xu, W. Wang, H. Yang, J. Wang, R. Li, S. Beck, J. Wang & X. Zhang.

PLoS Biology 9, 8 (2010) 11, e1000533, doi:10,1371, journal.pbio.100053.


285.Pig transgenesis by Sleeping Beauty DNA transposition.

J.E. Jakobsen, J. Li, P.M. Kragh, B. Moldt, L. Lin, Y. Liu, M. Schmidt, K.D. Winther, B.D. Schyth, I.E. Holm, G. Vajta, L. Bolund, H. Callesen, A.L. Jørgensen, A.L. Nielsen & J.G. Mikkelsen.

Transgenic Research 20, 3 (2011) 535-545, doi: 10.1007/s11248-010-9438-x.


286.High efficiency of BRCA1 knockout using rAAV-mediated gene targeting: developing a pig model for breast cancer.

Y. Luo, J. Li, Y. Liu, L. Lin, Y. Du, S. Li, H. Yang, G. Vajta, H. Callesen, L. Bolund & C.B. Sørensen.

Transgenic Research 20, 5 (2011) 975-988, doi: 10,1007/s11248-010-9472-8.


287.An update on targeted gene repair in mammalian cells: methods and mechanisms.

N.M. Jensen, T. Dalsgaard, M. Jakobsen, R.R. Nielsen, C.B. Sørensen,

L. Bolund & T.G. Jensen.

Journal of Biomedical Science 18 (2011) 10, doi: 10.1186/1423-0127-18-10.


288.Establishment of a pig fibroblast-derived cell line for locus-directed transgene expression in cell cultures and blastocysts.

J.E. Jakobsen, J. Li, B. Moldt, P. Kragh, H. Callesen, J.M. Hertz, L. Bolund, A.L. Jørgensen, J.G. Mikkelsen & A.L. Nielsen.

Molecular Biology Reports 38, 1 (2011) 151-161.


289.Identification of chromosome aberrations in sporadic microsatellite stable and unstable colorectal cancers using array comparative genomic hybridization.

T.D. Jensen, J. Li, K. Wang, J. Lindebjerg, S. Kølvraa, L. Bolund, A. Jakobsen, G. Bruun-Petersen, S. Li & D.G. Crüger.

Cancer Genetics & Cytogenetics 204, 2 (2011) 84-95.


290.Aseptic minimum volume vitrification technique for porcine parthenogenetically activated blastocysts.

L. Lin, Y. Du, X. Zhang, H. Yang, L. Bolund, H. Callesen & G. Vajta.

Cryo-Letters 32, 1 (2011) 62-68.


291.A Sleeping Beauty DNA transposon-based genetic sensor for functional screening of vitamin D3 analogues.

N.H. Staunstrup, N. Sharma, R.O. Bak, L. Svensson, T.K. Petersen, L. Aarenstrup, K. Kristiansen, L. Bolund & J.G. Mikkelsen.

BMC Biotechnology 11, 1 (2011) 33.


292.Mosaics and moles.

L. Sunde, I. Niemann, E.S. Hansen, J. Hindkjaer, B. Degn, U.B. Jensen & L. Bolund.

European Journal of Human Genetics 19, 10 (2011) 1026-1031, doi: 10.1038/ejhg.2011.93.


293.An improved PCR strategy for fast screening of specific and random integrations in rAAV-mediated gene targeted cell clones.

Y. Luo, L. Bolund & C.B. Sørensen.

BMC Research Notes 4, 1 (2011) 246.


294.DNA methylation in peripheral blood cells of pigs cloned by somatic cell nuclear transfer.

F. Gao, S. Li, L. Lin, J. Li, Y. Luo, X. Zhang, A.L. Nielsen & L.Bolund. Cellular Reprogramming 13, 4 (2011) 307-314.


295.Final Report “Pigs and Health” 2007-2011.

Aarhus University: Department of Biomedicine & Department of Clinical Medicine, Aarhus University: Department of Molecular Biology and Genetics & Department of Animal Science, University of Copenhagen: Department of Basic Animal and Veterinary Sciences, Technical University of Denmark, LEO Pharma, Ellegaard Göttingen Minipigs A/S, Pixigene A/S, Pig Research Centre, Danish Agriculture and Food Council.

C. Bendixen, L. Bolund, H. Callesen, C.B. Sørensen et al.

Højteknologifonden (2011) 29 pages.


296.Computational methods for epigenetic analysis: the protocol of computational analysis for modified methylation-specific digital karyotyping based on massively parallel sequencing.

J. Li, Q. Zhao & L. Bolund.

Methods in Molecular Biology 791 (2011) 313-328.


297.Comparison of gene expression and genome-wide DNA methylation profiling between phenotypically normal cloned pigs and conventionally bred controls.

G. Fei, Y. Luo, S. Li, J. Li, L. Lin, A.L. Nielsen, C.B, Sørensen, G. Vajta, J. Wang, X. Zhang, Y. Du, H. Yang, H. & L. Bolund.

PLoS One 6, 10 (2011) 13, doi: 10.1371/journal.pone.0025901.

.

298.[Production of porcine blastocysts expressed EGFP by handmade cloning] (in Chinese).

P. Zhang, Z. Yang, H. Dou, W. Li, B. Lu, L. Bolund, Y. Du, P. Tan & R. Ma.

Yi Chuan 33, 5 (2011) 527-32.


299.Design, recruitment, logistics, and data management of the GEHA (Genetics of Healthy Ageing) project.

A. Skytthe, S. Valensin, B. Jeune, E. Cevenini, F. Balard, M. Beekman, V. Bezrukov, H. Blanche, L. Bolund, K. Broczek, C. Carru, K. Christensen, L. Christiansen, J.C. Collerton, R. Cotichini, A.J.M. de Craen, S. Dato, K. Davies, G. De Benedictis, L. Deiana, F. Flachsbart, J. Gampe, C. Gilbault, E.S. Gonos, E. Haimes, A. Hervonen, M.A. Hurme, D. Janiszewska, M. Jylhä, T.B.L. Kirkwood, T. P. Kristensen, P. Laiho, A. Leon, A. Marchisio, R. Masciulli, A. Nebel, G. Passarino, G. Pelicci, L. Peltonen, M. Perola, M. Poulain, I.M. Rea, J. Remacle, JM., Robine, S. Schreiber, M. Scurti, F. Sevini, E. Sikora, A. Skouteri, P.E. Slagboom, L. Spazzafumo, M.A. Stazi, V. Toccaceli, O. Toussaint, O. Törnwall, J.W. Vaupel, K. Voutetakis, C. Franceschi & the GEHA consortium.

Experimental Gerontology 46, 11 (2011) 934-945.


300.Temporal dissection of tumorigenesis in primary cancers.

S. Durinck, C. Ho, N.J. Wang, W. Liao, L.R. Jakkula, E.A. Collisson, J. Pons, S. Chan, E.T. Lam, C. Chu, K. Park, S. Hong, J.S. Hur, N. Huh, I.M. Neuhaus, S.S. Yu, R.T. Grekin, T.M. Mauro, J.E. Cleaver, P. Kwok, P.E. Leboit, G. Getz, K. Cibulskis, J.C. Aster, H. Huang, E. Purdom, J. Li, L. Bolund, S.T. Arron, J.W. Gray, P.T. Spellman & R.J. Cho.

Cancer Drug Discovery and Development 1, 2 (2011) 137-143.


301.Structural variation in two human genomes mapped at single-nucleotide resolution by whole genome de novo assembly.

Y. Li, H. Zheng, R. Luo, H. Wu, H. Zhu, R. Li, H. Cao, B. Wu, S. Huang, H. Shao, H. Ma, F. Zhang, S. Feng, W. Zhang, H. Du, G. Tian, J. Li, X. Zhang, S. Li, L. Bolund, K. Kristiansen, A.J. de Smith, A.I.F. Blakemore, L.J.M. Coin, H. Yang, J. Wang & J. Wang.

Nature Biotechnology 29, 8 (2011) 723-730.


302.Development of transgenic cloned pig models of skin inflammation by DNA transposon-directed ectopic expression of human ß1 and a2 integrin.

N.H. Staunstrup, J. Madsen, M.D.N.L. Primo, J. Li, Y. Liu, P. Kragh, R. Li, M. Schmidt, S. Purup, F. Dagnæs-Hansen, L. Svensson, T.K. Petersen, H. Callesen, L. Bolund & J.G. Mikkelsen.

PLoS One 7, 5 (2012) 1-18.



303.Handmade cloned transgenic piglets expressing the nematode fat-1 gene.

P. Zhang, Y. Zhang, H. Dou, J. Yin, Y. Chen, X. Pang, G. Vajta, L. Bolund, Y. Du & R.Z. Ma.

Cellular Reprogramming 14, 3 (2012) 258-266.


304.Single-cell exome sequencing and monoclonal evolution of a JAK2-negative myeloproliferative neoplasm.

Y. Hou, L. Song, P. Zhu, B. Zhang, Y. Tao, X. Xu, F. Li, K. Wu, J. Liang, D. Shao, H. Wu, X. Ye, C. Ye, R. Wu, M. Jian, Y. Chen, W. Xie, R. Zhang, L. Chen, X. Liu, X. Yao, H. Zheng, C. Yu, Q. Li, Z. Gong, M. Mao, X. Yang, L. Yang, J. Li, W. Wang, Z. Lu, N. Gu, L. Goodman, L. Bolund, K. Kristiansen, J. Wang, H. Yang, Y. Li, X. Zhang & J. Wang.

Cell 148, 5 (2012) 873-885.


305.Single-cell exome sequencing reveals single-nucleotide mutation characteristics of a kidney tumor.

X. Xu, Y. Hou, X. Yin, L. Bao, A. Tang, L. Song, F. Li, S. Tsang, K. Wu, H. Wu, W. He, L. Zeng, M. Xing, R. Wu, H. Jiang, X. Liu, D. Cao, G. Guo, X. Hu, Y. Gui, Z. Li, W. Xie, X. Sun, M. Shi, Z. Cai, B. Wang, M. Zhong, J. Li, Z. Lu, N. Gu, X. Zhang, L. Goodman, L. Bolund, J. Wang, H. Yang, K. Kristiansen, M. Dean, Y. Li & J. Wang.

Cell 148, 5 (2012) 886-895.


306.Array comparative genomic hybridization of keratoacanthomas and squamous cell carcinomas: Different patterns of genetic aberrations suggest two distinct entities.

J. Li, K. Wang, F. Gao, T.D. Jensen, S.T. Li, P.M. Deangelis, S. Kølvraa, C. Proby, O. Forslund, L. Bolund & O.P.F. Clausen.

Journal of Investigative Dermatology 132, 8 (2012) 2060-2066.


307.Genetically modified pigs for biomedical research.

Y. Luo, L. Lin, L. Bolund, T.G. Jensen & C.B. Sørensen.

Journal of Inherited Metabolic Disease 35, 4 (2012) 695-713.


308.Targeted Genome Editing by Recombinant Adeno-Associated Virus (rAAV) Vectors for Generating Genetically Modified Pigs.

Y. Luo, E. Kofod-Olsen, R. Christensen, C.B. Sørensen & L. Bolund.

Journal of Genetics and Genomics 39, 6 (2012) 269-274.


309.Recombinant AAV-mediated homologous recombination in nuclear donor cells for SCNT cloning in pigs.

C.B. Søresen, Y. Luo & L. Bolund.

Transgenic Research 21, 4 (2012) 904-905.


310.Hypercholesterolemic minipigs created by liver-specific expression of a PCSK9 gain-of-function mutant.

J.F. Bentzon, C.B. Sørensen, R.H. Al-Mashhadi, P.M. Kragh, L.B. Nielsen,

J. Li, L. Lin, Y. Liu, B. Moldt, M. Schmidt, T. Thim, G. Vajta, S. Purup,

L. Bolund, H. Callesen, J.G. Mikkelsen & E. Falk.

Transgenic Research 21, 4 (2012) 910-911.


311.Pig gene knockout by rAAV-mediated homologous recombination: comparison of BRCA1 gene knockout efficiency in Yucatan and Göttingen fibroblasts with slightly different target sequences.

Y. Luo, L. Bolund & C.B. Sørensen.

Transgenic Research 21 (2012) 671-676, doi: 10.1007/s11248-011-9563-1.


312. Analyses of pig genomes provide insight into porcine demography and evolution.

M.A.M. Groenen, A.L. Archibald, H. Uenishi, C.K. Tuggle, Y. Takeuchi, M.F. Rothschild, C. Rogel-Gaillard, C. Park, D. Milan, H-J. Megens, S. Li, D.M. Larkin, H. Kim, L.A.F. Frantz, M. Caccamo, H. Ahn, B.L. Aken,

A. Anselmo, C. Anthon, L. Auvil, B. Badaoui, C.W. Beattie, C. Bendixen,

D. Berman, F. Blecha, J. Blomberg, L. Bolund, M. Bosse, S. Botti, B. Zhan, M. Bystrom, B. Capitanu, D. Carvalho-Silva, P. Chardon, C. Chen, R. Cheng, S-H. Choi, W. Chow, R.C. Clark, C. Clee, R.P.M.A. Crooijmans,

H.D. Dawson, P. Dehais, F. De Sapio, B. Dibbits, N. Drou, Z-Q. Du,

K. Eversole, J. Fadista, S. Fairley, T. Faraut, G.J. Faulkner, K.E. Fowler,

M. Fredholm, E. Fritz, J.G.R. Gilbert, E. Giuffra, J. Gorodkin, D.K. Griffin,

J.L. Harrow, A. Hayward, K. Howe, Z-L Hu, S.J. Humphray, T. Hunt,

H. Hornshøj, J-T. Jeon, P. Jern, M. Jones, J. Jurka, H. Kanamori,

R. Kapetanovic, J. Kim, J-H. Kim, K-W. Kim, T-H. Kim, G. Larson, K. Lee, K-T. Lee, R. Leggett, H.A. Lewin, Y. Li, W. Liu, J.E. Loveland, Y. Lu,

J.K. Lunney, J. Ma, O. Madsen, K. Mann, L. Matthews, S. McLaren,

T. Morozumi, M.P. Murtaugh, J. Narayan, D.T. Nguyen, P. Ni, S-J. Oh,

S. Onteru, F. Panitz, E-W. Park, H-S. Park, G. Pascal, Y. Paudel, M. Perez-Enciso, R. Ramirez-Gonzalez, J.M. Reecy, S. Rodriguez-Zas, G.A. Rohrer,

L. Rund, Y. Sang, K. Schachtschneider, J.G. Schraiber, J. Schwartz, L. Scobie, C. Scott, S. Searle, B. Servin, B.R. Southey, G. Sperber, P. Stadler,

J.V. Sweedler, H. Tafer, B. Thomsen, R. Wali, J. Wang, J. Wang, S. White,

X. Xu, M. Yerle, G. Zhang, J. Zhang, J. Zhang, S. Zhao, J. Rogers,

C. Churcher & L.B. Schook.

Nature 491, 7424, (2012) 393-398.


313.Gene Expression Responses to FUS, EWS, and TAF15 Reduction and Stress Granule Sequestration Analyses Identifies FET-Protein Non-Redundant Functions.

J. Blechingberg, Y. Luo, L. Bolund, C.K. Damgaard & A.L.Nielsen.

PLoS One 7, 9 (2012) e46251.


314. Mosaic moles and non-familial biparental moles are not caused by mutations in NLRP7, NLRP2 or C6orf221.

L. Andreasen, L. Bolund, I. Niemann, E.S. Hansen & L. Sunde.

Molecular Human Reproduction 18, 12 (2012) 593-8.


315.Novel Methods in the Study of the Breast Cancer Genome:

Towards a Better Understanding of theDisease of Breast Cancer.

J. Li, X. Lin, N. Brünner, H. Yang & L. Bolund.

Journal of Cancer Therapy 3 (2012) 797-809.


316. Single-cell sequencing analysis characterizes common and cell-lineage-specific mutations in a muscle-invasive bladder cancer.

Y. Li, X. Xu, L. Song, Y. Hou, Z. Li, S. Tsang, F. Li, K. McGee, K. Wu,

H. Wu, X. Ye, G. Li, L. Wang, B. Zhang, J. Liang, W. Xie, R. Wu, H. Jiang, X. Liu, C.Yu, H. Zheng, M. Jian, L. Nie, L. Wan, M. Shi, X. Sun, A. Tang,

G. Guo, Y. Gui, Z. Cai, J. Li, W. Wang, Z. Lu, X. Zhang, L. Bolund,

K. Kristiansen, J. Wang, H. Yang, M. Dean & J.Wang.

GigaScience 1, 12 (2012) doi:10.1186/2047-217X-1-12.


317.The sequence and analysis of a Chinese pig genome.

X. Fang, Y. Mu, Z. Huang, Y. Li, L. Han, Y. Zhang, Y. Feng, Y. Chen,

X. Jiang, W. Zhao, X. Sun, Z. Xiong, L. Yang, H. Liu, D. Fan, L. Mao,

L. Ren, C. Liu, J. Wang, K. Li, G. Wang, S. Yang, L. Lai, G. Zhang, Y. Li,

J. Wang, L. Bolund, H. Yang, J. Wang, S. Feng, S. Li & Y. Du.

GigaScience 1, 16 (2012) doi:10.1186/2047-217X-1-16.


318. Genetic design of pigs as experimental models in the combat between chronic diseases and healthy aging.

L. Bolund.

Reproduction, Fertility and Development 25, 1 (2013) 321.


319.SOAPindel: E?cient identi?cation of indels from short paired reads.

S. Li, R. Li, H. Li, J. Lu, Y. Li, L. Bolund, M.H. Schierup & J. Wang. Genome Research 23 (2013) 195-200.


320. Exome sequencing-driven discovery of coding polymorphisms associated with common metabolic phenotypes.

A. Albrechtsen, N. Grarup, Y. Li, T.H. Sparsø, G. Tian, H. Cao, T. Jiang,

S.Y. Kim, T.S. Korneliussen, Q. Li, C. Nie, R. Wu, L. Skotte, A.P. Morris,

C. Ladenvall, S. Cauchi, A. Stancáková, G. Andersen, A. Astrup, K. Banasik, A.J. Bennett, L. Bolund, G. Charpentier, Y. Chen, J.M. Dekker, A.S.F. Doney,

M. Dorkhan, T. Forsen, T.M. Frayling, C.J. Groves, Y. Gui, G. Hallmans,

A.T. Hattersley, K. He, G.A. Hitman, J. Holmkvist, S. Huang, H. Jiang,

X. Jin, J.M. Justesen, K. Kristiansen, J. Kuusisto, M. Lajer, O. Lantieri, W, Li, H. Liang, Q. Liao, X. Liu, T. Ma, X. Ma, M.P. Manijak, M. Marre,

J. Mokrosinski, A.D. Morris, B. Mu, A.A. Nielsen, G. Nijpels, P. Nilsson,

C.N.A. Palmer, N.W. Rayner, F. Renström, R. Ribel-Madsen, N. Robertson,

O. Rolandsson, P. Rossing, T. Schwartz, P.E. Slagboom, M. Sterner, M. Tang, L. Tarnow, T. Tuomi, E. Van't Riet, N. van Leeuwen, T.V. Varga,

M.A. Vestmar, M. Walker, B. Wang, Y. Wang, H. Wu, F. Xi, L. Yengo,

C. Yu, X. Zhang, J. Zhang, Q. Zhang, W. Zhang, H. Zheng, Y. Zhou,

D. Altshuler, L.M. 't Hart, P.W. Franks, B. Balkau, P. Froguel, M.I, McCarthy, M. Laakso, L. Groop, C. Christensen, I. Brandslund, T. Lauritzen, D. Witte, D. R. , Linneberg, A. R. , Jørgensen, T. , Hansen, T. , Wang, J. , Nielsen, R. , O. Pedersen & D.E.S.I.R. Study Group.

Diabetologia Praktyczna. 56, 2 (2013) 298-310.


321. Familial hypercholesterolemia and atherosclerosis in cloned minipigs created by DNA transposition of a human PCSK9 gain-of-function mutant.

R.H. Al-Mashhadi, C.B. Sørensen, P. Kragh, C. Christoffersen, M.B. Mortensen, L.P. Tolbod, T. Thim, Y. Du, J. Li, Y. Liu, B. Moldt, M. Schmidt, G. Vajta, T. Larsen, S. Purup, L. Bolund, L.B. Nielsen, H. Callesen, E. Falk, J.G. Mikkelsen & J.F. Bentzon.

Science Translational Medicine 5 (2013) 166 166ra1.

doi:10.1126/scitranslmed.3004853.

A lentiviral vector-based genetic sensor system for comparative analysis of permeability and activity of vitamin D3 analogues in xenotransplanted human skin.
N.H. Staunstrup, R.O. Bak, Y. Cai, L. Svensson, A.T. Petersen, C. Rosada,  K. Stenderup, L. Bolund. & J.G. Mikkelsen.
Experimental Dermatology Online 22, 3 (2013) 178-183.

323. Tetraploidy in hydatidiform moles.
L. Sundvall, H. Lund, I. Niemann, U.B. Jensen, L. Bolund & L. Sunde.
Human Reproduction 28, 7 (2013) 2010-2020.

324. Effects of high hydrostatic pressure on genomic expression profiling of porcine parthenogenetic activated and cloned embryos.
L, Lin, Y. Luo, P. Sørensen, H. Prætorius, G. Vajta, H. Callesen, C. Pribenszky, L. Bolund & T.N. Kristensen,
Reproduction, Fertility and Development (2013) http://dx.doi.org/10.1071/RD13037.

325. Methylation specific Multiplex Ligation-dependent Probe Amplification: Utility for prenatal diagnosis of parental origin in human triploidy.
M.W. Joergensen, A.A. Rasmussen, I. Niemann, J. Hindkjaer, I. Agerholm, L. Bolund, S. Kolvraa & L. Sunde.
Prenatal Diagnosis (2013) doi: 10.1002/pd.4206.

326. A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds.
L. Zou, Y. Luo, M. Chen, G. Wang, M. Ding, C.C. Petersen, R. Kang, F. Dagnaes-Hansen, Y. Zeng, N. Lv, Q. Ma, D.Q.S. Le, F. Besenbacher, L. Bolund, T.G. Jensen, J. Kjems, W.T. Pu & C. Bünger.
Scientific Reports 3: 2243 (2013) doi: 10.1038/srep02243.

327. Novel Methods in the Study of the Breast Cancer Genome: Towards a Better Understanding of the Disease of Breast Cancer.
 J. Li, X. Lin, N.A. Brünner, H. Yang & L. Bolund.
Journal of Cancer Therapy 3 (2013) 797-809.

328. Development of transgenic minipigs with expression of antimorphic human cryptochrome 1.
 H. Liu, Y. Li, Q. Wei, C. Liu, L. Bolund, G. Vajta, H. Dou, W. Yang, Y. Xu, J. Luan, J. Wang, H. Yang, N.H. Staunstrup & Y. Du.
PloS one 8, No. 10 (2013) e76098.

329. Methylation specific Multiplex Ligation-dependent Probe Amplification: utility for prenatal diagnosis of parental origin in human triploidy.
 M.W. Joergensen, A.A. Rasmussen, I. Niemann, J. Hindkjaer, I. Agerholm, L. Bolund, S. Kolvraa & L. Sunde, L.
Prenatal Diagnosis 33, No.12 (2013) 1131-1136.

330. NLRP7 or KHDC3L genes and the etiology of molar pregnancies and recurrent miscarriage.
 L. Andreasen, O.B. Christiansen, I. Niemann, L. Bolund & L. Sunde.
Molecular Human Reproduction 19, No. 11 (2013) 773-781.

331. How to classify the oldest old according to their health status: a study on 1160 subjects belonging to 552 90+ Italian sib-ships characterized by familial longevity recruited within the GEHA EU Project.
 E. Cevenini, R. Cotichini, M.A. Stazi, V. Toccaceli, M. Scurti, V. Mari, M. Berardelli, G. Passarino, B. Jeune & C. Franceschi;
 GEHA Project Consortium: L. Bolund.
Mechanisms of Ageing and Development 134, No. 11-12 (2013) 560-569.

332. Integrative analyses of gene expression and DNA methylation profiles in breast cancer cell line models of tamoxifen-resistance indicate a potential role of cells with stem-like properties.
 X. Lin, J. Li, G. Yin, Q. Zhao, D. Elias, A.E. Lykkesfeldt, J. Stenvang, N. Brünner, J. Wang, H. Yang, L. Bolund & H.J. Ditzel.
 Breast Cancer Research (Online Edition) 15, No. 6 (2013) p. R119.

333. The co-occurrence of mtDNA mutations on different oxidative phosphorylation subunits, not detected by haplogroup analysis, affects human longevity and is population specific.
 N. Raule, F. Sevini, S. Li, A. Barbieri, F. Tallaro, L. Lomartire, D. Vianello, A. Montesanto, J.S. Moilanen, V. Bezrukov, H. Blanché, A. Hervonen, K. Christensen, L. Deiana, E.S. Gonos, T.B.L. Kirkwood, P. Kristensen, A. Leon, P.G. Pelicci, M. Poulain, I.M. Rea, J. Remacle, J.M. Robine, S. Schreiber, E. Sikora, P.E. Slagboom, L. Spazzafumo, M.A. Stazi, O. Toussaint, J.W. Vaupel, G. Rose, K. Majamaa, M. Perola, T.E. Johnson, L. Bolund, H. Yang, G. Passarino & C. Franceschi.
 Aging Cell 13, No. 3 (2014) 401-407.

334. Targeted Porcine Genome Engineering with TALENs.
 Y. Luo, L. Lin, M.M. Golas, C.B. Sørensen & L. Bolund.
 Somatic Genome Manipulation: Advances, Methods and Applications (2014) XXXX.

335. Osteogenesis of Human Induced Pluripotent Stem Cells Derived Mesenchymal Stem Cells on hydroxyapatite contained Nanofibers.
 R. Kang, Y. Luo, L. Zou, L. Xie, H. Lysdahl, X. Jiang, L. Bolund, M. Chen, F. Besenbacher & C.E. Bünger.
 R S C Advances 4, No. 11 (2014) 5734-5739.

336. Efficient genome engineering using rAAV and TALENs in pigs.
 Y. Luo, B.M. Viuff, L. Lin, R. Li, M. Schmidt, S.G. Rønn, H.D. Pedersen, H. Callesen, C.B. Sørensen, N. Brünner, T. Klein & L. Bolund.
 Transgenic Research 23, No. 1 (2014) 189-190.

337. Effects of high hydrostatic pressure on genomic expression profiling of porcine parthenogenetic activated and cloned embryos.
 L. Lin, Y. Luo, P. Sørensen, H. Prætorius, G. Vajta, H. Callesen, C. Pribenszky, L. Bolund & T.N. Kristensen.
 Reproduction, Fertility and Development 26 (2014) 469-484.

338. Variation and association to diabetes in 2000 full mtDNA sequences mined from an exome study in a Danish population.
 S. Li, S. Besenbacher, Y. Li, K. Kristiansen, N. Grarup, A. Albrechtsen, T.H. Sparsø, T.S. Korneliussen, T. Hansen, J. Wang, R. Nielsen, O. Pedersen, L. Bolund & M.H. Schierup.
 European Journal of Human Genetics 22 (2014) 1040-1045.

339. Altered cleavage patterns in human tripronuclear embryos and their association to fertilization method: A time-lapse study.
 M.W. Joergensen, I. Agerholm, J. Hindkjaer, L. Bolund, L. Sunde, H.J. Ingerslev &  K. Kirkegaard.
 Journal of Assisted Reproduction and Genetics 31 (2014) 435-442.

340. Triploid pregnancies, genetic and clinical features of 158 cases.
 M.W. Joergensen, I. Niemann, A.A. Rasmussen, J. Hindkjaer, I. Agerholm, L. Bolund & L. Sunde.
 American Journal of Obstetrics and Gynecology 210 (2014) XXXX.

341. Health status and 6 years survival of 552 90+ Italian sib-ships recruited within the EU Project GEHA (GEnetics of Healthy Ageing)
 E. Cevenini, R. Cotichini, M.A. Stazi, V. Toccaceli, M.G. Palmas, M. Capri, F. De Rango, S. Dato, G. Passarino, B. Jeune & C. Franceschi;
 GEHA Project Consortium: L. Bolund.
 Age (Dordrecht, Netherlands) 36, No. 2 (2014) 949-966.

342. Efficient construction of rAAV-based gene targeting vectors by Golden Gate cloning.
 Y. Luo, L. Lin, L. Bolund & C.B. Sørensen.
 BioTechniques 56, No. 5 (2014) 263-268.

343. GxE Interactions Between FOXO Genotypes and Tea Drinking Significantly Affect Cognitive Disability at Advanced Ages in China.
 Y. Zeng, H. Chen, T. Ni, R. Ruan, L. Feng, C. Nie, L. Cheng, Y. Li, W. Tao, J. Gu, K.C. Land, A. Yashin, Q. Tan, Z. Yang, L. Bolund, H. Yang, E. Hauser, C.D. Willcox, B.J. Willcox, X-L.Tian & J.W.Vaupel.
 The journals of gerontology: Series A, Biological sciences and medical sciences (2014) doi: 10.1093/gerona/glu060.

Database submissions outside the international Human Genome Project and Swine Genome Sequencing Consortium:


1.Homo sapiens mRNA for nucleoporin 155.

X. Zhang, H. Yang, M.J. Corydon, L. Song, L. Ding, L. Dai, J. Korenberg, X. Chen, J. Laporte, N. Gregersen, S. Kølvraa, E. Niebuhr, G. Liu & L. Bolund.

EMBL Nucleotide Sequence Data Base. Accession number AJ007558 (1998).


2.163797 bp human genomic DNA sequence: Human BAC clone djn 085o06 containing the NUP155 gene from chromosome 5p13.

X. Zhang, J. Yu, S. Hu, W. Dong, N. Gregersen, L. Bolund & H. Yang.

GenBank Submission bankit279995 1 of 1 (1999) 1-91.


3.Fugu rubripes nucleoporin 153 (NUP153) mRNA, complete cds.

X. Zhang, C. Chen, J. Bao, N. Gregersen, H. Wang & L. Bolund.

GenBank Submission bankit355024 (2000).


4.Fugu rubripes DNA sequence from cosmid 78K09 containing NUP153.

X. Zhang C. Chen, J. Bao, G. Zhang, J. Wang, D. Wu, W. Dong, S. Hu, J. Wang, J. Yu, N. Gregersen, H. Yang & L. Bolund.

GenBank Submission bankit355033 (2000).


5.Homo sapiens mRNA for ClpX-like protein.

T.J. Corydon, M. Wilsbech, C. Jespersgaard, B.S. Andresen, A.D. Børglum, S. Pedersen, L. Bolund, N. Gregersen & P. Bross.

GenBank/EMBL accession number AJ006267 (2000).


6.Mus musculus partial mRNA for CLPX.

T.J. Corydon, M. Wilsbech, C. Jespersgaard, B.S. Andresen, A.D. Børglum, S. Pedersen, L. Bolund, N. Gregersen & P. Bross.

GenBank/EMBL accession number AJ276991 (2000).


7.Homo sapiens partial CLPX gene, exon 1 and joined cds (exons 1-14).

T.J. Corydon, M. Wilsbech, C. Jespersgaard, B.S. Andresen, A.D. Børglum, S. Pedersen, L. Bolund, N. Gregersen & P. Bross.

GenBank/EMBL accession number AJ276980 (2000).


8.A 0.66x coverage pig genome survey based on shotgun sequencing.

R. Wernersson, M.H. Schierup, F.G. Jørgensen, J. Garodkin, F. Panitz,

H-H. Stærfeldt, O.F. Christensen, T. Mailund, H. Hjortshøj, A. Klein, Jun Wang, B. Liu, S. Hu, W. Dong, W. Li, G.K-S. Wong, J. Yu, Jian Wang, C. Bendixen, M. Fredholm, S. Brunak, H. Yang & L. Bolund.

NCBI Trace Repository: Center name: “SDJVP”; Project name: “Sino-Danish Pig Genome Project” (2005).



Patents:


1.Method and composition for treating hyperproliferative skin diseases using 6-aminopurine cytokinins.

Treating psoriasis with 6-(substd. amino)purine cytokinin(s) – induces cell differentiation, and is also effective against e.g. basal cell carcinoma and malignant melanoma.

L. Bolund, P.K.A. Jensen & P. Bjerring.

United States Patent, Number 5,021,422, June 4, 1991.

WO9220340-A; EP584062-A; US5021422-A, 1991-185211.


2.Treatment of hyper-proliferative skin diseases e.g. by seborpheic keratosis – by administration e.g. topically to the skin, of a 6 substd.-amino-purine cytokinin e.g. kinetin.

L. Bolund, P.K.A. Jensen & P. Bjerring.

US5164394-A, 1992-407177.


3.In situ Branch Capture Assay (IN SITU BCA) for mutations.

L. Bolund & J.W. Gray.

Submitted to the UCSF Patent Office. UC Case No.:93-004-1.


4.Detection of polymorphisms and mutations.

S. Kølvraa, Xiaohui Chen Nielsen & L. Bolund.

PA 2002 00908.


5.Cell Nuclear Transfer comprises fusing at least one cytoplast with the donor cell or membrane surrounded cell nucleus.

Y. Du, G.Vajta, P.M. Kragh & L. Bolund

PA 2005 01256. WO2007028396-A2, 2007-255230.


6.Handmade Cloning.

AU-2005-634-108, PCT/DK2006/000498.


7.Integration of a genetic sensor system.

AU-2006-634-169, PCT/DK2009/050225.


8.Apolipoprotein E knockout and LDL receptor knockdown Yucatan minipigs.

AU-2007-634-174, PCT/DK2008/050055.


9.Generation of Cloned Master Pigs.

AU-2007-634-176, PCT/DK2008/050058.


10.Psoriasis Pig Model.

AU-2007-634-178, PCT/DK2008/050059.


11.Breast Cancer Pig Model.

AU-2007-634-179, PCT/DK2008/050057.