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Knud Erik Bach Knudsen

Whole Grain Consumption Increases Gastrointestinal Content of Sulfate-Conjugated Oxylipins in Pigs − A Multicompartmental Metabolomics Study

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  • Anne Krog Ingerslev
  • İbrahim Karaman, Department of Epidemiology and Biostatistics, School of Public Health, Imperial College London, St. Mary’s Campus, Norfolk Place, W2 1PG London, United Kingdom, Danmark
  • Murat Bağcıoğlu, Department of Mathematical Sciences and Technology (IMT), Norwegian University of Life Sciences, Drøbakveien 31, 1432 Ås, Norway, Norge
  • Achim Kohler, Department of Mathematical Sciences and Technology (IMT), Norwegian University of Life Sciences, Drøbakveien 31, 1432 Ås, Norway, Nofima AS, Osloveien 1, 1430 Ås, Norway, Norge
  • Peter Kappel Theil
  • Knud Erik Bach Knudsen
  • Mette Skou Hedemann
The effects of increased intake of dietary fiber as either arabinoxylan (AX) or resistant starch (RS) compared to a typical low dietary fiber Western-style diet (WSD) on the metabolomics responses was studied in gastrointestinal content and tissue, peripheral plasma and urine using a multicompartmental non-targeted LC-MS approach in pigs. Both unsupervised and supervised multivariate analyses were used to study differences between the intervention groups, revealing significant effects of the dietary intervention on gastrointestinal contents and urine metabolites. Consumption of an AX rich diet composed of whole grain and enzyme treated wheat bran increased gastrointestinal content of novel sulfate- and acetic acid conjugated oxylipins, and urinary secretion of phenolic compounds. Furthermore, potential gastrointestinal and urinary biomarkers for consumption of resistant starch, whole grain rye, and a low dietary fiber intake were detected using multi block analysis. This study provides insight into microbial fermentation products in the gastrointestinal tract, and suggests a potential role in sulfate conjugation of metabolites on the bioavailability of ingested nutrients. In addition, the use of multicompartmental metabolomics offers information about the correlations between the compartments of the digestive system, providing additional insight into effects of increased whole grain intake.
TidsskriftJournal of Proteome Research
Sider (fra-til)3095-3110
Antal sider16
StatusUdgivet - 7 aug. 2015

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