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Knud Erik Bach Knudsen

Fecal metabolome and microbiota composition in obese Gottingen Minipigs as influecned by source of carbohydrates

Publikation: KonferencebidragPosterForskningpeer review

The objective of this study was to explore the effects of ad libitum feeding of two high-fat diets supplemented with either high amylose maize starch (HIMA) or fructose on the fecal metabolome and microbiota composition. These effects were examined in obese Göttingen Minipigs as a model for human metabolic disorders.
Thirty Göttingen Minipigs were fed with two experimental diets for five months, and fecal material was sampled three times during the dietary intervention. Non-targeted liquid chromatography-mass spectrometry was used to explore metabolite differences, short-chain fatty acids (SCFAs) were quantified by gas chromatography, and bacterial diversity was assessed by 16S rRNA gene sequencing. Sequencing data were analyzed using QIIME 2, and together with metabolomics and SCFAs data a sparse multi-block partial least squares regression model was calculated.
Higher proportions of Bacteroidetes were found in the fecal contents of animals fed HIMA compared to that fed fructose together with higher quantities of total organic acids and total SCFAs. Furthermore, concentrations of acetate and acetate+propionate+butyrate were positively associated with the Ruminococcus genus and increased levels of several dicarboxylic acids (2,4-dimethyladipic acid, 3,3-methylglutaric acid) and pantothenic acid. Minipigs fed fructose contained higher butyrate levels, which were closely associated with enrichments in Roseburia, Clostridiaceae, Turicibacter, and Ruminococcus (Lachnospiraceae family). Bacteria from the Coprococcus, Blautia or Coprococcus genera were associated with a higher intensity of enterolactone (m/z 297.1135; [M+Cl] m/z 333.0902). Overall, we observed that different carbohydrate sources induced a contrasting stimulation of the microbial taxa which further reflected in a divergent fecal metabolome profile.
StatusUdgivet - 2019
BegivenhedMetabolomics 2019: 15th Anual Conference of the Metabolomics Society - World Forum, The Hague, Holland
Varighed: 23 jun. 201927 jun. 2019


KonferenceMetabolomics 2019: 15th Anual Conference of the Metabolomics Society
LokationWorld Forum
ByThe Hague

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