Aarhus University Seal / Aarhus Universitets segl

Kai Finster

Effect of Aerosolization and Drying on the Viability of Pseudomonas syringae Cells

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Effect of Aerosolization and Drying on the Viability of Pseudomonas syringae Cells. / Alsved, Malin; Holm, Stine; Christiansen, Sigurd; Smidt, Mads; Ling, Meilee; Boesen, Thomas; Finster, Kai; Bilde, Merete; Londahl, Jakob; Santl-Temkiv, Tina.

I: Frontiers in Microbiology, Bind 9, 3086, 18.12.2018.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

APA

CBE

MLA

Vancouver

Author

Alsved, Malin ; Holm, Stine ; Christiansen, Sigurd ; Smidt, Mads ; Ling, Meilee ; Boesen, Thomas ; Finster, Kai ; Bilde, Merete ; Londahl, Jakob ; Santl-Temkiv, Tina. / Effect of Aerosolization and Drying on the Viability of Pseudomonas syringae Cells. I: Frontiers in Microbiology. 2018 ; Bind 9.

Bibtex

@article{330a744434094925ae267754d8de549e,
title = "Effect of Aerosolization and Drying on the Viability of Pseudomonas syringae Cells",
abstract = "Airborne dispersal of microorganisms influences their biogeography, gene flow, atmospheric processes, human health and transmission of pathogens that affect humans, plants and animals. The extent of their impact depends essentially on cell-survival rates during the process of aerosolization. A central factor for cell-survival is water availability prior to and upon aerosolization. Also, the ability of cells to successfully cope with stress induced by drying determines their chances of survival. In this study, we used the ice-nucleation active, plant pathogenic Pseudomonas syringae strain R10.79 as a model organism to investigate the effect of drying on cell survival. Two forms of drying were simulated: drying of cells in small droplets aerosolized from a wet environment by bubble bursting and drying of cells in large droplets deposited on a surface. For drying of cells both in aerosol and surface droplets, the relative humidity (RH) was varied in the range between 10 and 90{\%}. The fraction of surviving cells was determined by live/dead staining followed by flow cytometry. We also evaluated the effect of salt concentration in the water droplets on the survival of drying cells by varying the ionic strength between 0 and 700 mM using NaCl and sea salt. For both aerosol and surface drying, cell survival increased with decreasing RH (p <0.01), and for surface drying, survival was correlated with increasing salt concentration (p <0.001). Imaging cells with TEM showed shrunk cytoplasm and cell wall damage for a large fraction of aerosolized cells. Ultimately, we observed a 10-fold higher fraction of surviving cells when dried as aerosol compared to when dried on a surface. We conclude that the conditions, under which cells dry, significantly affect their survival and thus their success to spread through the atmosphere and colonize new environments as well as their ability to affect atmospheric processes.",
keywords = "bioaerosols, aerosolization, Pseudomonas syringae, drying, bubble bursting, ice nucleation activity, RELATIVE-HUMIDITY, PROPIDIUM IODIDE, BACTERIA, WATER, SURVIVAL, AIR, MICROORGANISMS, ATMOSPHERE, MECHANISM, CLOUDS",
author = "Malin Alsved and Stine Holm and Sigurd Christiansen and Mads Smidt and Meilee Ling and Thomas Boesen and Kai Finster and Merete Bilde and Jakob Londahl and Tina Santl-Temkiv",
year = "2018",
month = "12",
day = "18",
doi = "10.3389/fmicb.2018.03086",
language = "English",
volume = "9",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S.A",

}

RIS

TY - JOUR

T1 - Effect of Aerosolization and Drying on the Viability of Pseudomonas syringae Cells

AU - Alsved, Malin

AU - Holm, Stine

AU - Christiansen, Sigurd

AU - Smidt, Mads

AU - Ling, Meilee

AU - Boesen, Thomas

AU - Finster, Kai

AU - Bilde, Merete

AU - Londahl, Jakob

AU - Santl-Temkiv, Tina

PY - 2018/12/18

Y1 - 2018/12/18

N2 - Airborne dispersal of microorganisms influences their biogeography, gene flow, atmospheric processes, human health and transmission of pathogens that affect humans, plants and animals. The extent of their impact depends essentially on cell-survival rates during the process of aerosolization. A central factor for cell-survival is water availability prior to and upon aerosolization. Also, the ability of cells to successfully cope with stress induced by drying determines their chances of survival. In this study, we used the ice-nucleation active, plant pathogenic Pseudomonas syringae strain R10.79 as a model organism to investigate the effect of drying on cell survival. Two forms of drying were simulated: drying of cells in small droplets aerosolized from a wet environment by bubble bursting and drying of cells in large droplets deposited on a surface. For drying of cells both in aerosol and surface droplets, the relative humidity (RH) was varied in the range between 10 and 90%. The fraction of surviving cells was determined by live/dead staining followed by flow cytometry. We also evaluated the effect of salt concentration in the water droplets on the survival of drying cells by varying the ionic strength between 0 and 700 mM using NaCl and sea salt. For both aerosol and surface drying, cell survival increased with decreasing RH (p <0.01), and for surface drying, survival was correlated with increasing salt concentration (p <0.001). Imaging cells with TEM showed shrunk cytoplasm and cell wall damage for a large fraction of aerosolized cells. Ultimately, we observed a 10-fold higher fraction of surviving cells when dried as aerosol compared to when dried on a surface. We conclude that the conditions, under which cells dry, significantly affect their survival and thus their success to spread through the atmosphere and colonize new environments as well as their ability to affect atmospheric processes.

AB - Airborne dispersal of microorganisms influences their biogeography, gene flow, atmospheric processes, human health and transmission of pathogens that affect humans, plants and animals. The extent of their impact depends essentially on cell-survival rates during the process of aerosolization. A central factor for cell-survival is water availability prior to and upon aerosolization. Also, the ability of cells to successfully cope with stress induced by drying determines their chances of survival. In this study, we used the ice-nucleation active, plant pathogenic Pseudomonas syringae strain R10.79 as a model organism to investigate the effect of drying on cell survival. Two forms of drying were simulated: drying of cells in small droplets aerosolized from a wet environment by bubble bursting and drying of cells in large droplets deposited on a surface. For drying of cells both in aerosol and surface droplets, the relative humidity (RH) was varied in the range between 10 and 90%. The fraction of surviving cells was determined by live/dead staining followed by flow cytometry. We also evaluated the effect of salt concentration in the water droplets on the survival of drying cells by varying the ionic strength between 0 and 700 mM using NaCl and sea salt. For both aerosol and surface drying, cell survival increased with decreasing RH (p <0.01), and for surface drying, survival was correlated with increasing salt concentration (p <0.001). Imaging cells with TEM showed shrunk cytoplasm and cell wall damage for a large fraction of aerosolized cells. Ultimately, we observed a 10-fold higher fraction of surviving cells when dried as aerosol compared to when dried on a surface. We conclude that the conditions, under which cells dry, significantly affect their survival and thus their success to spread through the atmosphere and colonize new environments as well as their ability to affect atmospheric processes.

KW - bioaerosols

KW - aerosolization

KW - Pseudomonas syringae

KW - drying

KW - bubble bursting

KW - ice nucleation activity

KW - RELATIVE-HUMIDITY

KW - PROPIDIUM IODIDE

KW - BACTERIA

KW - WATER

KW - SURVIVAL

KW - AIR

KW - MICROORGANISMS

KW - ATMOSPHERE

KW - MECHANISM

KW - CLOUDS

U2 - 10.3389/fmicb.2018.03086

DO - 10.3389/fmicb.2018.03086

M3 - Journal article

VL - 9

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 3086

ER -