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Kai Finster

Disguised as a sulfate reducer: Growth of the Deltaproteobacterium Desulfurivibrio alkaliphilus by Sulfide Oxidation with Nitrate

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Disguised as a sulfate reducer: Growth of the Deltaproteobacterium Desulfurivibrio alkaliphilus by Sulfide Oxidation with Nitrate. / Thorup, Casper; Schramm, Andreas; Findlay, Alyssa Jean Lehsau; Finster, Kai; Schreiber, Lars.

I: mBio (Online), Bind 8, Nr. 4, e00671-17, 18.07.2017.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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@article{cf6fd9ea37114d9aafe7bc4df96eeeba,
title = "Disguised as a sulfate reducer: Growth of the Deltaproteobacterium Desulfurivibrio alkaliphilus by Sulfide Oxidation with Nitrate",
abstract = "This study demonstrates that the deltaproteobacterium Desulfurivibrioalkaliphilus can grow chemolithotrophically by coupling sulfide oxidation to the dissimilatory reduction of nitrate and nitrite to ammonium. Key genes of known sulfide oxidation pathways are absent from the genome of D. alkaliphilus. Instead, the genome contains all of the genes necessary for sulfate reduction, including a gene fora reductive-type dissimilatory bisulfite reductase (DSR). Despite this, growth by sulfatereduction was not observed. Transcriptomic analysis revealed a very high expressionlevel of sulfate-reduction genes during growth by sulfide oxidation, while inhibition experiments with molybdate pointed to elemental sulfur/polysulfides as intermediates. Consequently, we propose that D. alkaliphilus initially oxidizes sulfideto elemental sulfur, which is then either disproportionated, or oxidized by a reversalof the sulfate reduction pathway. This is the first study providing evidence that areductive-type DSR is involved in a sulfide oxidation pathway. Transcriptome sequencing further suggests that nitrate reduction to ammonium is performed by anovel type of periplasmic nitrate reductase and an unusual membrane-anchored nitrite reductase.",
author = "Casper Thorup and Andreas Schramm and Findlay, {Alyssa Jean Lehsau} and Kai Finster and Lars Schreiber",
year = "2017",
month = "7",
day = "18",
doi = "10.1128/ mBio.00671-17",
language = "English",
volume = "8",
journal = "mBio (Online)",
issn = "2150-7511",
publisher = "American Society for Microbiology",
number = "4",

}

RIS

TY - JOUR

T1 - Disguised as a sulfate reducer: Growth of the Deltaproteobacterium Desulfurivibrio alkaliphilus by Sulfide Oxidation with Nitrate

AU - Thorup, Casper

AU - Schramm, Andreas

AU - Findlay, Alyssa Jean Lehsau

AU - Finster, Kai

AU - Schreiber, Lars

PY - 2017/7/18

Y1 - 2017/7/18

N2 - This study demonstrates that the deltaproteobacterium Desulfurivibrioalkaliphilus can grow chemolithotrophically by coupling sulfide oxidation to the dissimilatory reduction of nitrate and nitrite to ammonium. Key genes of known sulfide oxidation pathways are absent from the genome of D. alkaliphilus. Instead, the genome contains all of the genes necessary for sulfate reduction, including a gene fora reductive-type dissimilatory bisulfite reductase (DSR). Despite this, growth by sulfatereduction was not observed. Transcriptomic analysis revealed a very high expressionlevel of sulfate-reduction genes during growth by sulfide oxidation, while inhibition experiments with molybdate pointed to elemental sulfur/polysulfides as intermediates. Consequently, we propose that D. alkaliphilus initially oxidizes sulfideto elemental sulfur, which is then either disproportionated, or oxidized by a reversalof the sulfate reduction pathway. This is the first study providing evidence that areductive-type DSR is involved in a sulfide oxidation pathway. Transcriptome sequencing further suggests that nitrate reduction to ammonium is performed by anovel type of periplasmic nitrate reductase and an unusual membrane-anchored nitrite reductase.

AB - This study demonstrates that the deltaproteobacterium Desulfurivibrioalkaliphilus can grow chemolithotrophically by coupling sulfide oxidation to the dissimilatory reduction of nitrate and nitrite to ammonium. Key genes of known sulfide oxidation pathways are absent from the genome of D. alkaliphilus. Instead, the genome contains all of the genes necessary for sulfate reduction, including a gene fora reductive-type dissimilatory bisulfite reductase (DSR). Despite this, growth by sulfatereduction was not observed. Transcriptomic analysis revealed a very high expressionlevel of sulfate-reduction genes during growth by sulfide oxidation, while inhibition experiments with molybdate pointed to elemental sulfur/polysulfides as intermediates. Consequently, we propose that D. alkaliphilus initially oxidizes sulfideto elemental sulfur, which is then either disproportionated, or oxidized by a reversalof the sulfate reduction pathway. This is the first study providing evidence that areductive-type DSR is involved in a sulfide oxidation pathway. Transcriptome sequencing further suggests that nitrate reduction to ammonium is performed by anovel type of periplasmic nitrate reductase and an unusual membrane-anchored nitrite reductase.

U2 - 10.1128/ mBio.00671-17

DO - 10.1128/ mBio.00671-17

M3 - Journal article

VL - 8

JO - mBio (Online)

JF - mBio (Online)

SN - 2150-7511

IS - 4

M1 - e00671-17

ER -