Jesper Dahlgaard

Lektor

Curriculum Vitae

Personal information: Born: 1/8-1967. Place: Denmark. Living in Aarhus with Mette and our children.

 

Key interests: Molecular Biology and Medical Sciences; Cancer diagnostics and prognostics, Micro Array analyses (global DNA/RNA analysis); Genetics and Genomics; Molecular Pathology; Metabolism: Diabetes & Stem cell research; Statistics; Pedagogy, Teaching and Positive Psychology

 

Skills: MicroArray analysis (mRNA, miRNA, SNPs), PCR; RT-QPCR, ELISA, Bioinformatics; Statistics; Managing of clinical trials; Writing and publishing scientific papers; Grant applications; Supervision; Teaching

       

Educational background:

2011 “Positive Psychology – Individual strengths and wellbeing”, one semester, Aarhus University.

99-01 “Organisation and Psychology”, four semesters (bachelor/sidefag: Cognitive psychology, Developmental and personality psychology, Educational psychology, Social psychology, and, Organizational psychology), Institute for Communication, Aalborg University.

1997 Ph.D in biology from the Faculty of Natural Sciences, Aarhus University.

1994 M.sc. in biology from the Faculty of Natural Sciences, Aarhus University.

1986 Matematisk-naturfaglig student from Marselisborg Gymnasium.

 

Course activity:

09 Nøglen til det gode salg. Tre dages kursus hos Ladegaards A/S, Virum.

09 FDA Quality System Regulations on medical devices & regulatory approval process, Charlottenlund

 

Positions held:

09- Guest Researcher, Institute of Pathology, University of Aarhus, Denmark

08-11 Senior Scientists, Head of Clinical Trials and Development, Medical Prognosis Institute, Hørsholm, Denmark

06-08 Senior Scientist, Department of Haematology, Aarhus University Hospital /Aalborg Hospital Science and Innovation Center

04-06 Lecturer at School of Medical laboratory Technology Aarhus (now VIA University College) teaching molecular biology, genetics, biochemistry, anatomy and physiology.

01-04 Research Associate Professor, participated in establishing Human MicroArray Centre in Torben Kruses lab., University of Southern Denmark/Odense University Hospital.

98-01 Research Assistant Professor at the Department of Ecology and Genetics, Institute of Biological sciences, University of Aarhus.

97-98 Research Assistant Professor at CTB, Institute of Zoology, University of Copenhagen.

 

Visiting scientist:

1994 Two month visit to Gerdien de Jong at Vakgroep populatie- en evolutiebilogi at the University of Utrecht, NL.

1995 Eight month visit to Ary Hoffmann, School of Genetics, La Trobe University, AU.

 


Conferences and travels:

2000 Application of Array Technology in Research and Diagnostics, Aarhus University Hospital, Skejby.

2001 BioChips. IBC, Munich, D.

2002 Application of Array Technology in Research and Diagnostics, Aarhus University Hospital, Skejby.

2003 Affymetrix User Group Meeting, Amsterdam, NL.

2003 XIX International Congress of Genetics, Melbourne, Australia.

2006 Danish Cancer Society XII symposium 25-27 Sept.

2007 Gene Expression Profiling - European Roundtable, Heidelberg, Germany.

2007 Forty-ninth American Society of Hematology (ASH) Annual Meeting, Atlanta, USA.

2009 13th World Congress in Lung Cancer, San Francisco, 2009.

 

Invited speaker and pro-offered paper sessions:

2009   13th World Congress in Lung Cancer, San Francisco, 2009.

2009  Workshop on Biomarkers and Companion Diagnostics. CELS, Malmö University Hospital

Malmö, Sweden

 

 

Referee: Referee for seven international - and one Danish journal.

 

Editorial:

-Editor at the Aarhus (DK) based journal Aktuelt Naturvidenskab: http://www.aktuelnat.au.dk

-Associate Editor for BioMed Central’s open access journal, BMC Research Notes

Supervisor activity:

-Torsten Nygård Kristensen, M.Sc. 2002. Inbreeding and its effect on the expression of hsp70 and stress resistance in Drosophila - implications for conservation biology.

-Pernille Saarup, M.Sc. 2002.  The role of Hsp70 and Hsp90 expression in heat induced male sterility in Drosophila.

-Freyja S. Eiríksdóttir, M.Sc. student 2003-2005. Development of small sample protocols for studying gene expression using micro arrays.

-Kirsten Jochumsen, MD, Ph.D student 2004-2005. Gene expression profiles as prognostic markers in woman with ovarian cancer.

- Niels Fritzner, Ph.D student. 2001-2006. Development of DNA-chips for analyses of candidate genes involved in Human Hereditary Diseases.

-Vibe Skov, Ph.D student 2003-2006. Gene expression profiling by DNA chips and subsequent bioinformatic analysis for identification of genes and biochemical pathways associated with type 2 diabetes.

-Anders Petersen, Ph.D student 2007-2008. MicroRNA targets in Diffuse Large B-Cell Lymphoma.

-Sara Elung-Jensen, Bachelor student in Biotechnology and Engineering (AAU) 2007-2008. RNA quality in biobanked paraffin embedded cancer tissue.

- In addition several project students at Aarhus University, Copenhagen University and School of Medical laboratory Technology Aarhus

 

Activity as opponent (examiner):

- Censor on master thesis ‘Oxygen tension effects on factors involved in development of Hepatitis B virus associated liver tumors’ by Birna Berndsen, BMB, University of Southern Denmark. 2005.

- Opponent on PhD thesis’ Implementation of cDNA Microarrays for the Study of Porcine Gene Expression’ by Per Horn, Danish Institute of Agricultural Sciences/Aarhus University Hospital Skejby. 2006.

- Opponent on PhD thesis ‘Microarray based studies of cell death, a method development and application study’ ’by Niels Magnusson, Molecular Diagnostic Laboratory (MDL)/Aarhus University Hospital Skejby. 2006.

- Opponent on PhD thesis ‘Investigating the effects of hypoxic preconditioning and reduced oxygen tension on in vitro chondrogenesis in human adipose tissue-derived stem cells (ASCs)’ by Linda Pilgaard, Laboratory for Stem Cell Research, Aalborg University. 2009.

- In addition several exams at School of Medical laboratory Technology Aarhus

 

Evaluation/assessment committees:

-Member of the committee (together with associate professor Trine Fink (chairman), AU, and, associate professor Morten Meyer, SDU) handling the positions of two assistant professorships in Stem Cell Research, Department of Health Science and Technology, Aalborg University. 2007.

 


Research network and collaborations:

Previous:

-The European Myeloma Network (EMN): http://www.myeloma-europe.org

-The Myeloma Stem Cell Network (MSCNET): http://www.myeloma-europe.org/mscnet

Current:

-Medical Prognosis Institute, Hørsholm: http://www.medical-prognosis.com/

-DBCG-TIBCAT, Danish Breast Cancer Cooperative Group (DBCG) Translational Research

Centre Towards Individualized Breast Cancer Treatment, workpackage 6: Predictive markers or marker profiles for chemotherapy sensitivity (WP. leader N. Brünner).

-Member of steering committee of CCS (Center for Cellebiologisk Sygdomsanalyse), Cancer Innovation consortium, Forsknings- og Innovationstyrelsen, http://www.fi.dk/tilskud/bevillingsoversigter/2009/innovationskonsortier-raadet-for-teknologi-og-innovation/ccs

- Roswell Park Cancer Institute and University of Alabama on a lung cancer trial (JD Head Clinical Trials)

- Department of Oncology (AUH) with Assoc. Prof. MD Marianne Nordsmark on the project “ANALYSIS OF THE CLINICAL IMPACT OF A NOVEL CHEMO- AND IRRADIATION SENSITIVITY PREDICTOR IN GASTROESOPHAGEAL CANCER” supported by the Danish Cancer Society

 

 Media

-TV2 NYHEDERNE 16/10/2007 – Ny metode til kræftbehandling (New method for cancer treatment)

-TV2 NORD 3/9/2007 - Individuel kræftbehandling (Individual cancer treatment): http://tv2nord.dk/default.asp?PageID=4&ProgrammePartID=112998&ProgrammeDate=03-09-2007&Start=00:07:57&Duration=00:01:42&FileName=030907.wmv

-Frontpage of the newspaper Nordjyske 29/9/2007 - Kamp mod kræft bliver mere præcis (The battle against cancer becomes more precise): http://www.aalborg.dk/Dansk/Visiting/Default.aspx?ctrl=424&data=28%2C2515110%2C531%2C4

 

Other activities:

Previous

-Member of the Faculty at Dansk Hæmatologisk Forskerskole: http://www.dahfo.dk/aalborg.php

Current

-National Faculty member at Forskerskolen i Metabolisme: http://www.metabolism-phd.dk/facu.html

 

Communications to Scientific Meetings (selected):

-Dahlgaard, J. Fritzner, N. and Kruse, T.A. 2001. Establishment of a microarray core facility at Odense University Hospital. APEX on microarrays. ESF workshop at Estonia Bio centre, Tartu.

-Dahlgaard, J., Tan, Q., Christiansen, L., Christensen, K., and  T. A. Kruse. 2003. Twin correlation on gene expression using high density oligonucleotide arrays. XIX International Congress of Genetics, Melbourne, Australia.

-Dahlgaard, J, Tan, Q., Kruse, T.A. and Kassem, M. 2003. Gene Expression Microarrays allow identification of genes and biochemical pathways involved in stem cells modified with the catalytic subunit of human telomerase (htert). XIX International Congress of Genetics, Melbourne, Australia.

- Thomassen M, Skov V, Eiriksdottir F, Tan Q, Jochumsen KM, Fritzner NG, Dahlgaard J, Brusgaard K, Kruse T and Bak M. 2006. Spotting and validation of a genome wide oligonucleotide chip with duplicate measurement of each gene. Poster session presented at Årsmøde i Dansk Selskab for Patologisk Anatomi og Cytologi, Odense.

- Dahlgaard J, Dybkaer K, Johnsen HE and Knudsen S. 2007. A Novel Chemo Sensitivity Index for Melphalan Based on Gene Expression Profiling (GEP). Forty-ninth American Society of Hematology (ASH) Annual Meeting, Atlanta, USA.

- Sai Yendamuri, Eric Kannisto, Jesper Dahlgaard, Steen Knudsen and Santosh K. Patnaik, MicroRNA (miRNA) expression for prediction of recurrence after surgical resection of patients with pathologic stage I non-small cell lung cancer (NSCLC). 13th World Congress in Lung Cancer, San Francisco, 2009.

-Knudsen S., Dahlgaard J, Mazin W, Zhang B and Chen J. Predicting response to TRAIL-induced Apoptosis. DBCGs translationelle årsmøde 2010. 

-Knudsen S., Dahlgaard J, Mazin W, Zhang B and Chen J. Method for Prediction of Response to Treatment of Breast Cancer. DBCGs translationelle årsmøde 2010. 

 

Conference and meeting abstracts (selected):

-Anders Petersen, Charlotte Mandrup, Preben Johansen, Thomas Urup, Jacob Madsen, Anne Dirks,  Anne Bukh, Karen Dybkær , Jesper Dahlgaard, Hans E Johnsen. MicroRNA expression in Diffuse Large B-Cell Lymphoma. MiRNA Europe, Cambridge, 2007

-Inger Lise Gade,Simon Damgaard Petersen,Anders Krog Vistisen, Anne Dirks Højfeldt, Preben Johansen, Hans E Johnsen, Martin Boegsted,Steen Knudsen, Jesper Dahlgaard, Pieter Sonneveld,Mark van Duin, Anne Bukh,and Karen Dybkær Sørensen.Gene expression profiling for individual diagnosis, prognosis and prediction by In silico analysis of microarray data in hematological malignancies. Dansk Hæmatologisk Selskab, Hindsgavl, 2009.

-M. Pøhl, O. Hansen, K.E. Olsen, S. Knudsen, J. Dahlgaard, T. Jensen, and H.J. Ditzel. Development of prognostic profiles in non-small-cell lung cancer. Danish Society for Clinical Oncology, 2009.

- Sai Yendamuri, Eric Kannisto, Jesper Dahlgaard, Steen Knudsen and Santosh K. Patnaik,  MicroRNA (miRNA) expression for prediction of recurrence after surgical resection of patients with pathologic stage I non-small cell lung cancer (NSCLC). 13th World Congress in Lung Cancer, San Francisco, 2009.

-S. Knudsen, J. Dahlgaard, T. Jensen, J. Timmons, L. Bullinger, R. Schlenk, H. Dohner, K. Metzeler, S. Bohlander, C. Buske. M. Bøgsted, K. Sørensen, M. Nyegaard, H. Johnsen. A Universal Predictor of Drug Response. ADAPT 2009: Accelerating Development & Advancing Personalized Therapy, Washington, DC, 2009.

 

Publications:

1) Dahlgaard, J., Krebs, R.A., and Loeschcke, V. Inbreeding and heat-shock tolerance in Drosophila buzzatii. Heredity 1995, 74, 157-163.

2) Dahlgaard, J. and Warncke, E. Seed set and germination in crosses conducted in nature within and between two geographically isolated populations of Saxifraga hirculus. Nordic Journal of Botany 1995, 15, 337-341.

3) Dahlgaard, J. and Loeschcke, V. Effects of inbreeding in three life stages of Drosophila buzzatii after embryos were exposed to a high temperature stress. Heredity 1997, 78, 410-416.

4) Loeschcke, V., Krebs, R. A., Dahlgaard, J. and Michalak, P. High temperature stress and the evolution of thermal resistance in Drosophila. In: R. Bijlsma and V. Loeschcke (eds): Stress, Adaptation and Evolution. Birkhäuser Verlag, Basel 1997, 175-191.

5) Dahlgaard, J., Loeschcke, V., Michalak, P. and Justesen, J. Induced thermotolerance and associated expression of the heat-shock protein HSP70 in adult Drosophila melanogaster. Functional Ecology 1998, 12, 786-793.

6) Kærsgaard, C. W., Vollmer, J., Dahlgaard, J. and Loeschcke, V. Heat induced male sterility

in Drosophila buzzatii: Genetic variation among populations for the duration of sterility. Drosophila Information Service 2000, 83, 5-8.

7) Dahlgaard, J. and Hoffmann, A. A. Stress resistance and environmental dependency of

inbreeding depression in Drosophila melanogaster: General versus Specific Effects. Conservation Biology 2000, 14, 1187-1192.

8) Sørensen, J. G., Dahlgaard, J. and Loeschcke, V. 2001. Genetic variation in natural populations of Drosophila buzzatii: Down regulation of Hsp70 expression and variation in heat stress traits indicating thermal adaptation. Functional Ecology, 15, 289-296.

9) Dahlgaard, J., Hasson, E. and Loeschcke, V. Behavioural differentiation in oviposition

activity in Drosophila buzzatii from highland and lowland populations in Argentina: plasticity or thermal adaptation? Evolution 2001, 55, 738-747.

10) Frydenberg J, Pertoldi C, Dahlgaard J, Loeschcke V. Genetic variation in original and colonizing Drosophila buzzatii populations analysed by microsatellite loci isolated with a new PCR screening method. Molecular Ecology. 2002, 11(2), 181-90.

11) Kristensen TN, Dahlgaard J, Loeschcke V. Inbreeding affects the Hsp70 expression level in two species of Drosophila even at benign temperaturesInbreeding affects Hsp70 expression level in two species of Drosophila even at benign temperatures. Evolutionary Ecology Research 2002, 4, 1209–1216.

12) Kristensen TN, Dahlgaard J, Loeschcke,V. Effects of inbreeding and environmental stress on fitness – using Drosophila buzzatii as a model organism. Conservation Genetics 2003, 4, 453–465.

13) Tan Q, Bathum L, Christiansen L, Benedictis G. De, Bellizzi D, Rose G, Frizner N, Dahlgaard J, Vach W, Vaupel J W, Yashin A I, Christensen K, Kruse T. Logistic regression models for polymorphic and antagonistic pleiotropic gene action on human aging and longevity.  Annals of Human Genetics 2003, 67 (6), 598-607

14) Vollmer, J. , Sarup, P.,  Kærsgaard, C. W., Dahlgaard, J.  and V. Loeschcke. Heat and cold-induced male sterility in Drosophila buzzatii: genetic variation among populations for the duration of sterility. Heredity 2004, 92 (3), 257-262.

15) Tan Q, Benedictis G De, Yashin AI, Bathum L, Christiansen L, Dahlgaard J, Fritzner N, Vach W, Vaupel JW, Christensen K, Kruse TA. Assessing genetic association with human survival at multi-allelic loci. Biogerontology 2004, 5, 89-97.

16) Sarup P, Dahlgaard J, Norup A-M, Jørgensen KT, Hebsgaard, MB and Losechcke V. Down regulation of Hsp70 expression level prolongs the duration of heat-induced male sterility in Drosophila buzzatii. Functional Ecology 2004, 18, 365-370.

17) Norry FM, Dahlgaard J and Loeschcke V. Quantitative trait loci affecting knock-down resistance to high temperature in Drosophila melanogaster. Molecular Ecology 2004, 13, 3585-3594.

18) Tan Q, Christensen K, Christiansen L, Frederiksen, H, Bathum L, Dahlgaard J and Kruse TA. Gene expression correlation observed in whole blood samples of aged Danish twins. Human Genetics 2005, 117(2-3), 267-74.

19) Tan Q, Dahlgaard J, Kassem M, Vach W and Kruse T. A non-parametric approach for identifying differentially expressed genes in factorial microarray experiments. Genome Biology, Vol. 6, Nr. 4, 2005, s. 5.

20) Thomassen M, Skov V, Eiriksdottir F, Tan Q, Jochumsen K, Fritzner N, Brusgaard K, Dahlgaard J and Kruse TA. Spotting and validation of a Genome wide oligonucleotide chip with duplicate measurement of each gene. Biochemical and Biophysical Research Communications. 2006, 344(4), 1111-20.

21) Jochumsen KM, Tan Q, Dahlgaard J, Kruse TA, Mogensen O. RNA quality and expression analysis of ovarian tumour tissue undergoing three thaw-freeze cycles. Experimental and Molecular Pathology, 2007, 82 (1), 95-102.

22) Abdallah BM, Boissy P, Tan Q, Dahlgaard J, Traustadottir GA, Kupisiewicz K, Laborda J, Delaisse J-M and Kassem M. DLK1/FA1 regulates the function of human bone marrow mesenchymal stem cells (HMSC) by modulating gene expression of pro-inflammatory cytokines and immune-response-related factors. The Journal of Biological Chemistry 2007, Mar 9; 282(10):7339-51.

23) Klitø NGF, Tan Q, Nyegaard M, Brusgaard K, Thomassen M,Skouboe C, Dahlgaard J, and Kruse TA. Arrayed Primer Extension in the ‘array of arrays’ Format: a Rational Approach for Microarray-Based SNP Genotyping. Genetic Testing 2007, 11(2).

24) Tan Q, Dahlgaard J, Abdallah BM, Vach W, Kassem M and Kruse T. A Bootstrap Correspondence Analysis for Factorial Microarray Experiments with Replication. In: I. Mandoiu and A. Zelikovsky (eds): International Symposium for Bioinformatics Research and
Applications
. Springer-Verlag, Berlin Heidelberg 2007, 73-84.

25) Dahlgaard J, MazinW, JensenT, PøhlM, BsharaW, HansenA, KanistoE, Hamilton-DutoitSJ, HansenO, HagerH, DitzelHJ, YendamuriS and Knudsen S. Analytical variables influencing the performance of a miRNA based laboratory assay for prediction of relapse in stage I non-small cell lung cancer (NSCLC). BMC Research Notes 2011, 4:424 (19 October 2011)

26) Chen J, Knudsen S, Mazin W, Dahlgaard J and Zhang B. A 71-gene signature of TRAIL sensitivity in cancer cells. Molecular Cancer Therapeutics, Published OnlineFirst October 25, 2011;doi:10.1158/1535-7163.MCT-11-0620.

 

Submitted

27) Expression of microRNAs in the NCI-60 cancer cell-lines. Patnaik SK, Dahlgaard J, Mazin W, Kannisto E, Knudsen S, Yendamuri S. Molecular Cancer Therapeutics, 2011.

 

To be submitted during 2011:

28) MazinW, DahlgaardJ, PøhlM, OlsenKE, HansenO, JensenT, Martinussen T, DitzelHJ and KnudsenS. Gene expression based prognosis of non-small cell lung cancer (NSCLC) - a retrospective blind validation study.

29) PøhlM, MartinussenT, MazinW, HansenO, OlsenKE, JensenT, DahlgaardJ, KnudsenS, DitzelHJ. Prognostic Gene Expression Signature Predict Survival in early stage Non-Small-Cell Lung Cancer.

 

Published conference and meeting abstracts.

1)Dahlgaard, J., Krebs, R.A., and Loeschcke, V. The interaction between thermal stress and inbreeding on survival in eggs and adults of Drosophila buzzatii. Hereditas 1994 (Abstract), 121, 215-216.

2) Abdallah BM, Tan Q, Dahlgaard J and Kassem M. Dlk1/Pref-1 maintains self-renewing undifferentiated state of human mesenchymal stem cells by upregulating cytokine and chemokine geneexpression. Journal of Bone and Mineral Research 2005 (Abstract), 20 (9): 361-361.

3) Dahlgaard J, Dybkaer K, Johnsen HE and Knudsen S. A Novel Chemo Sensitivity Index for Melphalan Based on Gene Expression Profiling (GEP). Blood 2007 (Abstract), 110 (11), 115B.

4) Mandrup, Charlotte ; Petersen, Anders ; Højfeldt, Anne Dirks ; Thomsen, H F ; Madsen, Jakob ; Dahlgaard, Jesper ; Johansen, P ; Bukh, Anne ; Dybkær, Karen ; Johnsen, Hans Erik. MicroRNA expression in nodal and extranodal Diffuse Large B-cell Lymphoma. 2008. 10th International conference on Malignant Lymphoma, Lugano, Schweiz, 4. juni 2008 - 7. juni 2008. S. 189 Annals of Oncology. June 2008 (Abstract). 19. supplement 4. Oxford University Press.

 

Newspapers and popular scientific journals:

1) Sørensen, J. G., Dahlgaard, J. and Loeschcke, V. Den moderne biologi kaster nyt lys over evolutionære processer. Aktuelt Naturvidenskab 2001, 1, 6-9.

2) Dahlgaard J, Fritzner N, Tan Q, Kruse TA. DNA-chips revolutionerer lægevidenskaben. Aktuel Naturvidenskab 2002, 1: 19-21 (http://www.dr.dk/videnskabold/artikler/aktnat/dnachips.asp).

3) Dahlgaard J, Jochumsen K, Cold S, Jensen ON, and Kruse TA. Ny teknologi i kampen mod kræft. Aktuelt Naturvidenskab, 2005.

4) Dahlgaard J, Jensen ON, and Kruse TA. Ny teknik i kampen mod kræft. Morgenavisen Jyllands-Posten (Indblik) 2005, december 4:10.

5) Krag Pedersen M, Johnsen HE, Dahlgaard J og Karen Dybkær Sørensen. Kræftstamceller

- på jagt efter ondets rod. Aktuelt Naturvidenskab, 2007.

Book reviews

1) Freddy Bugge Christiansen og Tom Fenchel: Evolution – the predictable randomness (in Danish).

Aarhus Universitetsforlag 2009. In: Aktuelt Naturvidenskab, 1, 2010.

2) Søren Rasmussen: In the beginning was Africa – stories about the development of life as seen through nature and humans in Africa (in Danish). Gyldendal 2011. In: Aktuelt Naturvidenskab, 5, 2011.