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Jens Christian Jensenius

Preparation and comparison of cytotoxic complexes formed between oleic acid and either bovine or human α-lactalbumin

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Standard

Preparation and comparison of cytotoxic complexes formed between oleic acid and either bovine or human α-lactalbumin. / Brinkmann, C R; Thiel, S; Larsen, M K et al.
I: Indian Journal of Dairy Science, Bind 94, Nr. 5, 2011, s. 2159-2170.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Harvard

Brinkmann, CR, Thiel, S, Larsen, MK, Petersen, TE, Jensenius, JC & Heegaard, CW 2011, 'Preparation and comparison of cytotoxic complexes formed between oleic acid and either bovine or human α-lactalbumin', Indian Journal of Dairy Science, bind 94, nr. 5, s. 2159-2170. https://doi.org/10.3168/jds.2010-3622

APA

Brinkmann, C. R., Thiel, S., Larsen, M. K., Petersen, T. E., Jensenius, J. C., & Heegaard, C. W. (2011). Preparation and comparison of cytotoxic complexes formed between oleic acid and either bovine or human α-lactalbumin. Indian Journal of Dairy Science, 94(5), 2159-2170. https://doi.org/10.3168/jds.2010-3622

CBE

Brinkmann CR, Thiel S, Larsen MK, Petersen TE, Jensenius JC, Heegaard CW. 2011. Preparation and comparison of cytotoxic complexes formed between oleic acid and either bovine or human α-lactalbumin. Indian Journal of Dairy Science. 94(5):2159-2170. https://doi.org/10.3168/jds.2010-3622

MLA

Brinkmann, C R et al. "Preparation and comparison of cytotoxic complexes formed between oleic acid and either bovine or human α-lactalbumin". Indian Journal of Dairy Science. 2011, 94(5). 2159-2170. https://doi.org/10.3168/jds.2010-3622

Vancouver

Brinkmann CR, Thiel S, Larsen MK, Petersen TE, Jensenius JC, Heegaard CW. Preparation and comparison of cytotoxic complexes formed between oleic acid and either bovine or human α-lactalbumin. Indian Journal of Dairy Science. 2011;94(5):2159-2170. doi: 10.3168/jds.2010-3622

Author

Brinkmann, C R ; Thiel, S ; Larsen, M K et al. / Preparation and comparison of cytotoxic complexes formed between oleic acid and either bovine or human α-lactalbumin. I: Indian Journal of Dairy Science. 2011 ; Bind 94, Nr. 5. s. 2159-2170.

Bibtex

@article{351c2cbc46a7461c961847d6877a3e9f,
title = "Preparation and comparison of cytotoxic complexes formed between oleic acid and either bovine or human α-lactalbumin",
abstract = "α-Lactalbumin is a ubiquitous calcium-binding milk protein with a well-characterized function in regulating the synthesis of lactose. An entirely different activity has been shown to occur when a complex is formed between calcium-free α-lactalbumin and oleic acid. This complex shows strong cytotoxic action against several cancer cells, and several mechanisms have been suggested to account for this cell-killing activity. Most studies have been performed using the human protein, but bovine α-lactalbumin shows similar activity. A new and simple 2-step method for purification of calcium-free α-lactalbumin has been developed, and the resulting highly purified preparation was used to generate a complex with oleic acid. Using 3 different cell lines and 2 types of cell viability assays, the bovine and human α-lactalbumin showed comparable cytotoxic activity. The effect was apparent after 15 min of incubation and was inhibited by the presence of fetal bovine serum or bovine serum albumin. The bovine protein might be a useful alternative to the human protein, but also raises the question whether cytotoxic activity could be generated in different kinds of food containing α-lactalbumin.",
keywords = "Animals, Cattle, Cell Count, Cell Line, Tumor, Culture Media, Serum-Free, Cytotoxins, HL-60 Cells, Humans, Lactalbumin, Milk, Milk, Human, Oleic Acid, Oleic Acids, Serum, U937 Cells",
author = "Brinkmann, {C R} and S Thiel and Larsen, {M K} and Petersen, {T E} and Jensenius, {Jens Christian} and Heegaard, {C W}",
note = "Copyright {\textcopyright} 2011 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.",
year = "2011",
doi = "10.3168/jds.2010-3622",
language = "English",
volume = "94",
pages = "2159--2170",
journal = "Indian Journal of Dairy Science",
issn = "0019-5146",
publisher = "Indian Dairy Association",
number = "5",

}

RIS

TY - JOUR

T1 - Preparation and comparison of cytotoxic complexes formed between oleic acid and either bovine or human α-lactalbumin

AU - Brinkmann, C R

AU - Thiel, S

AU - Larsen, M K

AU - Petersen, T E

AU - Jensenius, Jens Christian

AU - Heegaard, C W

N1 - Copyright © 2011 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

PY - 2011

Y1 - 2011

N2 - α-Lactalbumin is a ubiquitous calcium-binding milk protein with a well-characterized function in regulating the synthesis of lactose. An entirely different activity has been shown to occur when a complex is formed between calcium-free α-lactalbumin and oleic acid. This complex shows strong cytotoxic action against several cancer cells, and several mechanisms have been suggested to account for this cell-killing activity. Most studies have been performed using the human protein, but bovine α-lactalbumin shows similar activity. A new and simple 2-step method for purification of calcium-free α-lactalbumin has been developed, and the resulting highly purified preparation was used to generate a complex with oleic acid. Using 3 different cell lines and 2 types of cell viability assays, the bovine and human α-lactalbumin showed comparable cytotoxic activity. The effect was apparent after 15 min of incubation and was inhibited by the presence of fetal bovine serum or bovine serum albumin. The bovine protein might be a useful alternative to the human protein, but also raises the question whether cytotoxic activity could be generated in different kinds of food containing α-lactalbumin.

AB - α-Lactalbumin is a ubiquitous calcium-binding milk protein with a well-characterized function in regulating the synthesis of lactose. An entirely different activity has been shown to occur when a complex is formed between calcium-free α-lactalbumin and oleic acid. This complex shows strong cytotoxic action against several cancer cells, and several mechanisms have been suggested to account for this cell-killing activity. Most studies have been performed using the human protein, but bovine α-lactalbumin shows similar activity. A new and simple 2-step method for purification of calcium-free α-lactalbumin has been developed, and the resulting highly purified preparation was used to generate a complex with oleic acid. Using 3 different cell lines and 2 types of cell viability assays, the bovine and human α-lactalbumin showed comparable cytotoxic activity. The effect was apparent after 15 min of incubation and was inhibited by the presence of fetal bovine serum or bovine serum albumin. The bovine protein might be a useful alternative to the human protein, but also raises the question whether cytotoxic activity could be generated in different kinds of food containing α-lactalbumin.

KW - Animals

KW - Cattle

KW - Cell Count

KW - Cell Line, Tumor

KW - Culture Media, Serum-Free

KW - Cytotoxins

KW - HL-60 Cells

KW - Humans

KW - Lactalbumin

KW - Milk

KW - Milk, Human

KW - Oleic Acid

KW - Oleic Acids

KW - Serum

KW - U937 Cells

U2 - 10.3168/jds.2010-3622

DO - 10.3168/jds.2010-3622

M3 - Journal article

C2 - 21524506

VL - 94

SP - 2159

EP - 2170

JO - Indian Journal of Dairy Science

JF - Indian Journal of Dairy Science

SN - 0019-5146

IS - 5

ER -