Aarhus Universitets segl

Jens Christian Jensenius

Human H-ficolin inhibits replication of seasonal and pandemic influenza A viruses

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Human H-ficolin inhibits replication of seasonal and pandemic influenza A viruses. / Verma, Anamika; White, Mitchell; Vathipadiekal, Vinod et al.

I: Journal of Immunology, Bind 189, Nr. 5, 2012, s. 2478-87.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Verma, A, White, M, Vathipadiekal, V, Tripathi, S, Mbianda, J, Ieong, M, Qi, L, Taubenberger, JK, Takahashi, K, Jensenius, JC, Thiel, S & Hartshorn, KL 2012, 'Human H-ficolin inhibits replication of seasonal and pandemic influenza A viruses', Journal of Immunology, bind 189, nr. 5, s. 2478-87. https://doi.org/10.4049/jimmunol.1103786

APA

Verma, A., White, M., Vathipadiekal, V., Tripathi, S., Mbianda, J., Ieong, M., Qi, L., Taubenberger, J. K., Takahashi, K., Jensenius, J. C., Thiel, S., & Hartshorn, K. L. (2012). Human H-ficolin inhibits replication of seasonal and pandemic influenza A viruses. Journal of Immunology, 189(5), 2478-87. https://doi.org/10.4049/jimmunol.1103786

CBE

Verma A, White M, Vathipadiekal V, Tripathi S, Mbianda J, Ieong M, Qi L, Taubenberger JK, Takahashi K, Jensenius JC, et al. 2012. Human H-ficolin inhibits replication of seasonal and pandemic influenza A viruses. Journal of Immunology. 189(5):2478-87. https://doi.org/10.4049/jimmunol.1103786

MLA

Vancouver

Verma A, White M, Vathipadiekal V, Tripathi S, Mbianda J, Ieong M et al. Human H-ficolin inhibits replication of seasonal and pandemic influenza A viruses. Journal of Immunology. 2012;189(5):2478-87. doi: 10.4049/jimmunol.1103786

Author

Verma, Anamika ; White, Mitchell ; Vathipadiekal, Vinod et al. / Human H-ficolin inhibits replication of seasonal and pandemic influenza A viruses. I: Journal of Immunology. 2012 ; Bind 189, Nr. 5. s. 2478-87.

Bibtex

@article{ef094a79051b4af7a6cf90338299c6a1,
title = "Human H-ficolin inhibits replication of seasonal and pandemic influenza A viruses",
abstract = "The collectins have been shown to have a role in host defense against influenza A virus (IAV) and other significant viral pathogens (e.g., HIV). The ficolins are a related group of innate immune proteins that are present at relatively high concentrations in serum, but also in respiratory secretions; however, there has been little study of the role of ficolins in viral infection. In this study, we demonstrate that purified recombinant human H-ficolin and H-ficolin in human serum and bronchoalveolar lavage fluid bind to IAV and inhibit viral infectivity and hemagglutination activity in vitro. Removal of ficolins from human serum or bronchoalveolar lavage fluid reduces their antiviral activity. Inhibition of IAV did not involve the calcium-dependent lectin activity of H-ficolin. We demonstrate that H-ficolin is sialylated and that removal of sialic acid abrogates IAV inhibition, while addition of the neuraminidase inhibitor oseltamivir potentiates neutralization, hemagglutinin inhibition, and viral aggregation caused by H-ficolin. Pandemic and mouse-adapted strains of IAV are generally not inhibited by the collectins surfactant protein D or mannose binding lectin because of a paucity of glycan attachments on the hemagglutinin of these strains. In contrast, H-ficolin inhibited both the mouse-adapted PR-8 H1N1 strain and a pandemic H1N1 strain from 2009. H-ficolin also fixed complement to a surface coated with IAV. These findings suggest that H-ficolin contributes to host defense against IAV.",
keywords = "Animals, Antiviral Agents, Cell Line, Tumor, Cells, Cultured, Complement Fixation Tests, Dogs, Glycoproteins, Humans, Influenza A Virus, H1N1 Subtype, Influenza A Virus, H3N2 Subtype, Influenza, Human, Lectins, Mice, Neutralization Tests, Orthomyxoviridae Infections, Pandemics, Seasons, Viral Matrix Proteins, Virus Replication",
author = "Anamika Verma and Mitchell White and Vinod Vathipadiekal and Shweta Tripathi and Julvet Mbianda and Micheal Ieong and Li Qi and Taubenberger, {Jeffery K} and Kazue Takahashi and Jensenius, {Jens C} and Steffen Thiel and Hartshorn, {Kevan L}",
year = "2012",
doi = "10.4049/jimmunol.1103786",
language = "English",
volume = "189",
pages = "2478--87",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "5",

}

RIS

TY - JOUR

T1 - Human H-ficolin inhibits replication of seasonal and pandemic influenza A viruses

AU - Verma, Anamika

AU - White, Mitchell

AU - Vathipadiekal, Vinod

AU - Tripathi, Shweta

AU - Mbianda, Julvet

AU - Ieong, Micheal

AU - Qi, Li

AU - Taubenberger, Jeffery K

AU - Takahashi, Kazue

AU - Jensenius, Jens C

AU - Thiel, Steffen

AU - Hartshorn, Kevan L

PY - 2012

Y1 - 2012

N2 - The collectins have been shown to have a role in host defense against influenza A virus (IAV) and other significant viral pathogens (e.g., HIV). The ficolins are a related group of innate immune proteins that are present at relatively high concentrations in serum, but also in respiratory secretions; however, there has been little study of the role of ficolins in viral infection. In this study, we demonstrate that purified recombinant human H-ficolin and H-ficolin in human serum and bronchoalveolar lavage fluid bind to IAV and inhibit viral infectivity and hemagglutination activity in vitro. Removal of ficolins from human serum or bronchoalveolar lavage fluid reduces their antiviral activity. Inhibition of IAV did not involve the calcium-dependent lectin activity of H-ficolin. We demonstrate that H-ficolin is sialylated and that removal of sialic acid abrogates IAV inhibition, while addition of the neuraminidase inhibitor oseltamivir potentiates neutralization, hemagglutinin inhibition, and viral aggregation caused by H-ficolin. Pandemic and mouse-adapted strains of IAV are generally not inhibited by the collectins surfactant protein D or mannose binding lectin because of a paucity of glycan attachments on the hemagglutinin of these strains. In contrast, H-ficolin inhibited both the mouse-adapted PR-8 H1N1 strain and a pandemic H1N1 strain from 2009. H-ficolin also fixed complement to a surface coated with IAV. These findings suggest that H-ficolin contributes to host defense against IAV.

AB - The collectins have been shown to have a role in host defense against influenza A virus (IAV) and other significant viral pathogens (e.g., HIV). The ficolins are a related group of innate immune proteins that are present at relatively high concentrations in serum, but also in respiratory secretions; however, there has been little study of the role of ficolins in viral infection. In this study, we demonstrate that purified recombinant human H-ficolin and H-ficolin in human serum and bronchoalveolar lavage fluid bind to IAV and inhibit viral infectivity and hemagglutination activity in vitro. Removal of ficolins from human serum or bronchoalveolar lavage fluid reduces their antiviral activity. Inhibition of IAV did not involve the calcium-dependent lectin activity of H-ficolin. We demonstrate that H-ficolin is sialylated and that removal of sialic acid abrogates IAV inhibition, while addition of the neuraminidase inhibitor oseltamivir potentiates neutralization, hemagglutinin inhibition, and viral aggregation caused by H-ficolin. Pandemic and mouse-adapted strains of IAV are generally not inhibited by the collectins surfactant protein D or mannose binding lectin because of a paucity of glycan attachments on the hemagglutinin of these strains. In contrast, H-ficolin inhibited both the mouse-adapted PR-8 H1N1 strain and a pandemic H1N1 strain from 2009. H-ficolin also fixed complement to a surface coated with IAV. These findings suggest that H-ficolin contributes to host defense against IAV.

KW - Animals

KW - Antiviral Agents

KW - Cell Line, Tumor

KW - Cells, Cultured

KW - Complement Fixation Tests

KW - Dogs

KW - Glycoproteins

KW - Humans

KW - Influenza A Virus, H1N1 Subtype

KW - Influenza A Virus, H3N2 Subtype

KW - Influenza, Human

KW - Lectins

KW - Mice

KW - Neutralization Tests

KW - Orthomyxoviridae Infections

KW - Pandemics

KW - Seasons

KW - Viral Matrix Proteins

KW - Virus Replication

U2 - 10.4049/jimmunol.1103786

DO - 10.4049/jimmunol.1103786

M3 - Journal article

C2 - 22851708

VL - 189

SP - 2478

EP - 2487

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 5

ER -