Jens Christian Jensenius

Collectins, H-ficolin and LL-37 reduce influence viral replication in human monocytes and modulate virus-induced cytokine production

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review


  • Mitchell R White, Boston University
  • ,
  • Shweta Tripathi, Boston University
  • ,
  • Anamika Verma, Boston University
  • ,
  • Paul Kingma, University of Cincinnati
  • ,
  • Kazue Takahashi, 3 Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
  • ,
  • Jens Jensenius
  • Steffen Thiel
  • Guangshun Wang, 5 Department of Pathology and Microbiology, Nebraska Medical Center, Omaha, NE, USA.
  • ,
  • Erika C Crouch, Washington University St. Louis
  • ,
  • Kevan L Hartshorn, Boston University

Infiltrating activated monocytes are important mediators of damaging inflammation during influenza A virus (IAV) infection. We show that soluble respiratory proteins [collectins, surfactant proteins D (SP-D) and mannose binding lectin (MBL), H-ficolin and LL-37] inhibit replication of seasonal IAV in human monocytes. The collectins and H-ficolin also increased viral uptake by the cells, while LL-37 did not. H-ficolin was able to inhibit replication of the 2009 pandemic H1N1 strain (Cal09) in monocytes, but SP-D and LL-37 had significantly fewer inhibitory effects on this strain than on seasonal IAV. All of these proteins reduced IAV-induced TNF-α production, even in instances when viral replication was not reduced. We used modified recombinant versions of SP-D, MBL and ficolin to elucidate mechanisms through which these proteins alter monocyte interactions with IAV. We demonstrate the importance of the multimeric structure, and of binding properties of the lectin domain, in mediating antiviral and opsonic activity of the proteins. Hence, soluble inhibitors present in airway lining fluid may aid clearance of IAV by promoting monocyte uptake of the virus, while reducing viral replication and virus-induced TNF-α responses in these cells. However, SP-D and LL-37 have reduced ability to inhibit replication of pandemic IAV in monocytes.

TidsskriftInnate Immunity
Sider (fra-til)77-88
Antal sider12
StatusUdgivet - jan. 2017

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