Institut for Biomedicin

Jayashree Sahana

Short-Term Microgravity Influences Cell Adhesion in Human Breast Cancer Cells

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  • Mohamed Zakaria Nassef, Clinic for Plastic, Aesthetic and Hand Surgery, Otto von Guericke University, 39120 Magdeburg, Germany. christoph.buken@gmx.de.
  • ,
  • Sascha Kopp, Clinic for Plastic, Aesthetic and Hand Surgery, Otto von Guericke University, 39120 Magdeburg, Germany. christoph.buken@gmx.de.
  • ,
  • Daniela Melnik, Clinic for Plastic, Aesthetic and Hand Surgery, Otto von Guericke University, 39120 Magdeburg, Germany. christoph.buken@gmx.de.
  • ,
  • Thomas J Corydon
  • Jayashree Sahana
  • Marcus Krüger, Clinic for Plastic, Aesthetic and Hand Surgery, Otto von Guericke University, 39120 Magdeburg, Germany. christoph.buken@gmx.de.
  • ,
  • Markus Wehland
  • Thomas J Bauer, Clinic for Plastic, Aesthetic and Hand Surgery, Otto von Guericke University, 39120 Magdeburg, Germany. christoph.buken@gmx.de.
  • ,
  • Christian Liemersdorf, Institute of Aerospace Medicine, Department of Gravitational Biology, German Aerospace Center, 51147 Cologne, Germany.
  • ,
  • Ruth Hemmersbach, Institute of Aerospace Medicine, Department of Gravitational Biology, German Aerospace Center, 51147 Cologne, Germany.
  • ,
  • Manfred Infanger, Clinic for Plastic, Aesthetic and Hand Surgery, Otto von Guericke University, 39120 Magdeburg, Germany. christoph.buken@gmx.de.
  • ,
  • Daniela Grimm

With the commercialization of spaceflight and the exploration of space, it is important to understand the changes occurring in human cells exposed to real microgravity (r-µg) conditions. We examined the influence of r-µg, simulated microgravity (s-µg, incubator random positioning machine (iRPM)), hypergravity (hyper-g), and vibration (VIB) on triple-negative breast cancer (TNBC) cells (MDA-MB-231 cell line) with the aim to study early changes in the gene expression of factors associated with cell adhesion, apoptosis, nuclear factor "kappa-light-chain-enhancer" of activated B-cells (NF-κB) and mitogen-activated protein kinase (MAPK) signaling. We had the opportunity to attend a parabolic flight (PF) mission and to study changes in RNA transcription in the MDA-MB cells exposed to PF maneuvers (29th Deutsches Zentrum für Luft- und Raumfahrt (DLR) PF campaign). PF maneuvers induced an early up-regulation of ICAM1, CD44 and ERK1 mRNAs after the first parabola (P1) and a delayed upregulation of NFKB1, NFKBIA, NFKBIB, and FAK1 after the last parabola (P31). ICAM-1, VCAM-1 and CD44 protein levels were elevated, whereas the NF-κB subunit p-65 and annexin-A2 protein levels were reduced after the 31st parabola (P31). The PRKCA, RAF1, BAX mRNA were not changed and cleaved caspase-3 was not detectable in MDA-MB-231 cells exposed to PF maneuvers. Hyper-g-exposure of the cells elevated the expression of CD44 and NFKBIA mRNAs, iRPM-exposure downregulated ANXA2 and BAX, whereas VIB did not affect the TNBC cells. The early changes in ICAM-1 and VCAM-1 and the rapid decrease in the NF-κB subunit p-65 might be considered as fast-reacting, gravity-regulated and cell-protective mechanisms of TNBC cells exposed to altered gravity conditions. This data suggest a key role for the detected gravity-signaling elements in three-dimensional growth and metastasis.

OriginalsprogEngelsk
Artikelnummer5730
TidsskriftInternational Journal of Molecular Sciences
Vol/bind20
Nummer22
ISSN1661-6596
DOI
StatusUdgivet - 15 nov. 2019

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