Aarhus Universitet

Jayashree Sahana

Preparation of A Spaceflight: Apoptosis Search in Sutured Wound Healing Models

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DOI

  • Stefan Riwaldt
  • Monica Monici, ASA Campus Joint Laboratory, ASA Research Division, Department. of Experimental and Clinical Biomedical Sciences, University of Florence, 50121 Florence, Italy. monica.monici@unifi.it.
  • ,
  • Asbjørn Graver Petersen
  • Uffe Birk Jensen
  • Katja Evert, Institute for Pathology, University of Regensburg, 95053 Regensburg, Germany. katja.evert@klinik.uni-regensburg.de.
  • ,
  • Desiré Pantalone, Department of Critical Medicine and Surgery, University of Florence, 50134 Florence, Italy. desire.pantalone@unifi.it.
  • ,
  • Kirsten Utpatel, Institute for Pathology, University of Regensburg, 95053 Regensburg, Germany. kirsten.utpatel@klinik.uni-regensburg.de.
  • ,
  • Matthias Evert, Institute for Pathology, University of Regensburg, 95053 Regensburg, Germany. matthias.evert@klinik.uni-regensburg.de.
  • ,
  • Markus Wehland, Clinic and Policlinic for Plastic, Aesthetic and Hand Surgery, Otto von Guericke University, Leipziger Str. 44, 39120 Magdeburg, Germany. markus.wehland@med.ovgu.de.
  • ,
  • Marcus Krüger, Clinic and Policlinic for Plastic, Aesthetic and Hand Surgery, Otto von Guericke University, Leipziger Str. 44, 39120 Magdeburg, Germany. marcus.krueger@med.ovgu.de.
  • ,
  • Sascha Kopp, Clinic and Policlinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke-University, 39120 Magdeburg, Germany. sascha.kopp@med.ovgu.de.
  • ,
  • Sofie Frandsen
  • ,
  • Thomas Corydon
  • Jayashree Sahana
  • Johann Bauer, Max-Planck-Institute for Biochemistry Martinsried, 82152 Planegg, Germany. jbauer@biochem.mpg.de.
  • ,
  • Ronald Lützenberg, Clinic and Policlinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke-University, 39120 Magdeburg, Germany. ronald.luetzenberg@med.ovgu.de.
  • ,
  • Manfred Infanger, Clinic and Policlinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke-University, 39120 Magdeburg, Germany. manfred.infanger@med.ovgu.de.
  • ,
  • Daniela Grimm

To prepare the ESA (European Space Agency) spaceflight project "Wound healing and Sutures in Unloading Conditions", we studied mechanisms of apoptosis in wound healing models based on ex vivo skin tissue cultures, kept for 10 days alive in serum-free DMEM/F12 medium supplemented with bovine serum albumin, hydrocortisone, insulin, ascorbic acid and antibiotics at 32 °C. The overall goal is to test: (i) the viability of tissue specimens; (ii) the gene expression of activators and inhibitors of apoptosis and extracellular matrix components in wound and suture models; and (iii) to design analytical protocols for future tissue specimens after post-spaceflight download. Hematoxylin-Eosin and Elastica-van-Gieson staining showed a normal skin histology with no signs of necrosis in controls and showed a normal wound suture. TdT-mediated dUTP-biotin nick end labeling for detecting DNA fragmentation revealed no significant apoptosis. No activation of caspase-3 protein was detectable. FASL, FADD, CASP3, CASP8, CASP10, BAX, BCL2, CYC1, APAF1, LAMA3 and SPP1 mRNAs were not altered in epidermis and dermis samples with and without a wound compared to 0 day samples (specimens investigated directly post-surgery). BIRC5, CASP9, and FN1 mRNAs were downregulated in epidermis/dermis samples with and/or without a wound compared to 0 day samples. BIRC2, BIRC3 were upregulated in 10 day wound samples compared to 0 day samples in epidermis/dermis. RELA/FAS mRNAs were elevated in 10 day wound and no wound samples compared to 0 day samples in dermis. In conclusion, we demonstrate that it is possible to maintain live skin tissue cultures for 10 days. The viability analysis showed no significant signs of cell death in wound and suture models. The gene expression analysis demonstrated the interplay of activators and inhibitors of apoptosis and extracellular matrix components, thereby describing important features in ex vivo sutured wound healing models. Collectively, the performed methods defining analytical protocols proved to be applicable for post-flight analyzes of tissue specimens after sample return.

OriginalsprogEngelsk
TidsskriftInternational Journal of Molecular Sciences (Online)
Vol/bind18
Nummer12
ISSN1661-6596
DOI
StatusUdgivet - 3 dec. 2017

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