Aarhus Universitet

Jayashree Sahana

Pathway Analysis Hints Towards Beneficial Effects of Long-Term Vibration on Human Chondrocytes

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DOI

  • Ronald Lützenberg, Clinic for Plastic, Aesthetic and Hand Surgery, Otto-von Guericke-University, Magdeburg, Germany.
  • ,
  • Kendrick Solano, Clinic for Plastic, Aesthetic and Hand Surgery, Otto-von Guericke-University, Magdeburg, Germany.
  • ,
  • Christoph Buken, Clinic for Plastic, Aesthetic and Hand Surgery, Otto-von Guericke-University, Magdeburg, Germany.
  • ,
  • Jayashree Sahana
  • Stefan Riwaldt
  • Sascha Kopp, Clinic for Plastic, Aesthetic and Hand Surgery, Otto-von Guericke-University, Magdeburg, Germany.
  • ,
  • Marcus Krüger, Clinic for Plastic, Aesthetic and Hand Surgery, Otto-von Guericke-University, Magdeburg, Germany.
  • ,
  • Herbert Schulz, Cologne Center for Genomics (CCG), University of Cologne, Cologne, Germany.
  • ,
  • Kathrin Saar, Max-Delbrück-Center for Molecular Medicine, 13125 Berlin, Germany
  • ,
  • Norbert Huebner, Max-Delbrück-Center for Molecular Medicine, 13125 Berlin, Germany
  • ,
  • Ruth Hemmersbach, DLR, German Aerospace Centre, Institute of Aerospace Medicine, Gravitational Biology, Cologne, Germany.
  • ,
  • Johann Bauer, Max-Planck Institute for Biochemistry, Martinsried, Germany.
  • ,
  • Manfred Infanger, Clinic for Plastic, Aesthetic and Hand Surgery, Otto-von Guericke-University, Magdeburg, Germany.
  • ,
  • Daniela Grimm
  • Markus Wehland, Clinic for Plastic, Aesthetic and Hand Surgery, Otto-von Guericke-University, Magdeburg, Germany.

BACKGROUND/AIMS: Spaceflight negatively influences the function of cartilage tissue in vivo. In vitro human chondrocytes exhibit an altered gene expression of inflammation markers after a two-hour exposure to vibration. Little is known about the impact of long-term vibration on chondrocytes.

METHODS: Human cartilage cells were exposed for up to 24 h (VIB) on a specialised vibration platform (Vibraplex) simulating the vibration profile which occurs during parabolic flights and compared to static control conditions (CON). Afterwards, they were investigated by phase-contrast microscopy, rhodamine phalloidin staining, microarray analysis, qPCR and western blot analysis.

RESULTS: Morphological investigations revealed no changes between CON and VIB chondrocytes. F-Actin staining showed no alterations of the cytoskeleton in VIB compared with CON cells. DAPI and TUNEL staining did not identify apoptotic cells. ICAM-1 was elevated and vimentin, beta-tubulin and osteopontin proteins were significantly reduced in VIB compared to CON cells. qPCR of cytoskeletal genes, ITGB1, SOX3, SOX5, SOX9 did not reveal differential regulations. Microarray analysis detected 13 differentially expressed genes, mostly indicating unspecific stimulations. Pathway analyses demonstrated interactions of PSMD4 and CNOT7 with ICAM.

CONCLUSIONS: Long-term vibration did not damage human chondrocytes in vitro. The reduction of osteopontin protein and the down-regulation of PSMD4 and TBX15 gene expression suggest that in vitro long-term vibration might even positively influence cultured chondrocytes.

OriginalsprogEngelsk
TidsskriftCellular Physiology and Biochemistry
Vol/bind47
Nummer4
Sider (fra-til)1729-1741
Antal sider13
ISSN1015-8987
DOI
StatusUdgivet - jul. 2018

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