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Jayashree Sahana

Common Effects on Cancer Cells Exerted by a Random Positioning Machine and a 2D Clinostat

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Common Effects on Cancer Cells Exerted by a Random Positioning Machine and a 2D Clinostat. / Svejgaard, Benjamin; Wehland, Markus; Ma, Xiao; Kopp, Sascha; Sahana, Jayashree; Warnke, Elisabeth; Aleshcheva, Ganna; Hemmersbach, Ruth; Hauslage, Jens; Grosse, Jirka; Bauer, Johann; Corydon, Thomas Juhl; Islam, Tawhidul; Infanger, Manfred; Grimm, Daniela.

I: PLOS ONE, Bind 10, Nr. 8, 15.08.2015, s. e0135157.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Svejgaard, B, Wehland, M, Ma, X, Kopp, S, Sahana, J, Warnke, E, Aleshcheva, G, Hemmersbach, R, Hauslage, J, Grosse, J, Bauer, J, Corydon, TJ, Islam, T, Infanger, M & Grimm, D 2015, 'Common Effects on Cancer Cells Exerted by a Random Positioning Machine and a 2D Clinostat', PLOS ONE, bind 10, nr. 8, s. e0135157. https://doi.org/10.1371/journal.pone.0135157

APA

CBE

Svejgaard B, Wehland M, Ma X, Kopp S, Sahana J, Warnke E, Aleshcheva G, Hemmersbach R, Hauslage J, Grosse J, Bauer J, Corydon TJ, Islam T, Infanger M, Grimm D. 2015. Common Effects on Cancer Cells Exerted by a Random Positioning Machine and a 2D Clinostat. PLOS ONE. 10(8):e0135157. https://doi.org/10.1371/journal.pone.0135157

MLA

Vancouver

Author

Svejgaard, Benjamin ; Wehland, Markus ; Ma, Xiao ; Kopp, Sascha ; Sahana, Jayashree ; Warnke, Elisabeth ; Aleshcheva, Ganna ; Hemmersbach, Ruth ; Hauslage, Jens ; Grosse, Jirka ; Bauer, Johann ; Corydon, Thomas Juhl ; Islam, Tawhidul ; Infanger, Manfred ; Grimm, Daniela. / Common Effects on Cancer Cells Exerted by a Random Positioning Machine and a 2D Clinostat. I: PLOS ONE. 2015 ; Bind 10, Nr. 8. s. e0135157.

Bibtex

@article{6a6d6f765ef44cb6973a9ec185406fa6,
title = "Common Effects on Cancer Cells Exerted by a Random Positioning Machine and a 2D Clinostat",
abstract = "In this study we focused on gravity-sensitive proteins of two human thyroid cancer cell lines (ML-1; RO82-W-1), which were exposed to a 2D clinostat (CLINO), a random positioning machine (RPM) and to normal 1g-conditions. After a three (3d)- or seven-day-culture (7d) on the two devices, we found both cell types growing three-dimensionally within multicellular spheroids (MCS) and also cells remaining adherent (AD) to the culture flask, while 1g-control cultures only formed adherent monolayers, unless the bottom of the culture dish was covered by agarose. In this case, the cytokines IL-6 and IL-8 facilitated the formation of MCS in both cell lines using the liquid-overlay technique at 1g. ML-1 cells grown on the RPM or the CLINO released amounts of IL-6 and MCP-1 into the supernatant, which were significantly elevated as compared to 1g-controls. Release of IL-4, IL-7, IL-8, IL-17, eotaxin-1 and VEGF increased time-dependently, but was not significantly influenced by the gravity conditions. After 3d on the RPM or the CLINO, an accumulation of F-actin around the cellular membrane was detectable in AD cells of both cell lines. IL-6 and IL-8 stimulation of ML-1 cells for 3d and 7d influenced the protein contents of {\ss}1-integrin, talin-1, Ki-67, and beta-actin dose-dependently in adherent cells. The {\ss}1-integrin content was significantly decreased in AD and MCS samples compared with 1g, while talin-1 was higher expressed in MCS than AD populations. The proliferation marker Ki-67 was elevated in AD samples compared with 1g and MCS samples. The {\ss}-actin content of R082-W-1 cells remained unchanged. ML-1 cells exhibited no change in {\ss}-actin in RPM cultures, but a reduction in CLINO samples. Thus, we concluded that simulated microgravity influences the release of cytokines in follicular thyroid cancer cells, and the production of {\ss}1-integrin and talin-1 and predicts an identical effect under real microgravity conditions.",
author = "Benjamin Svejgaard and Markus Wehland and Xiao Ma and Sascha Kopp and Jayashree Sahana and Elisabeth Warnke and Ganna Aleshcheva and Ruth Hemmersbach and Jens Hauslage and Jirka Grosse and Johann Bauer and Corydon, {Thomas Juhl} and Tawhidul Islam and Manfred Infanger and Daniela Grimm",
year = "2015",
month = "8",
day = "15",
doi = "10.1371/journal.pone.0135157",
language = "English",
volume = "10",
pages = "e0135157",
journal = "P L o S One",
issn = "1932-6203",
publisher = "public library of science",
number = "8",

}

RIS

TY - JOUR

T1 - Common Effects on Cancer Cells Exerted by a Random Positioning Machine and a 2D Clinostat

AU - Svejgaard, Benjamin

AU - Wehland, Markus

AU - Ma, Xiao

AU - Kopp, Sascha

AU - Sahana, Jayashree

AU - Warnke, Elisabeth

AU - Aleshcheva, Ganna

AU - Hemmersbach, Ruth

AU - Hauslage, Jens

AU - Grosse, Jirka

AU - Bauer, Johann

AU - Corydon, Thomas Juhl

AU - Islam, Tawhidul

AU - Infanger, Manfred

AU - Grimm, Daniela

PY - 2015/8/15

Y1 - 2015/8/15

N2 - In this study we focused on gravity-sensitive proteins of two human thyroid cancer cell lines (ML-1; RO82-W-1), which were exposed to a 2D clinostat (CLINO), a random positioning machine (RPM) and to normal 1g-conditions. After a three (3d)- or seven-day-culture (7d) on the two devices, we found both cell types growing three-dimensionally within multicellular spheroids (MCS) and also cells remaining adherent (AD) to the culture flask, while 1g-control cultures only formed adherent monolayers, unless the bottom of the culture dish was covered by agarose. In this case, the cytokines IL-6 and IL-8 facilitated the formation of MCS in both cell lines using the liquid-overlay technique at 1g. ML-1 cells grown on the RPM or the CLINO released amounts of IL-6 and MCP-1 into the supernatant, which were significantly elevated as compared to 1g-controls. Release of IL-4, IL-7, IL-8, IL-17, eotaxin-1 and VEGF increased time-dependently, but was not significantly influenced by the gravity conditions. After 3d on the RPM or the CLINO, an accumulation of F-actin around the cellular membrane was detectable in AD cells of both cell lines. IL-6 and IL-8 stimulation of ML-1 cells for 3d and 7d influenced the protein contents of ß1-integrin, talin-1, Ki-67, and beta-actin dose-dependently in adherent cells. The ß1-integrin content was significantly decreased in AD and MCS samples compared with 1g, while talin-1 was higher expressed in MCS than AD populations. The proliferation marker Ki-67 was elevated in AD samples compared with 1g and MCS samples. The ß-actin content of R082-W-1 cells remained unchanged. ML-1 cells exhibited no change in ß-actin in RPM cultures, but a reduction in CLINO samples. Thus, we concluded that simulated microgravity influences the release of cytokines in follicular thyroid cancer cells, and the production of ß1-integrin and talin-1 and predicts an identical effect under real microgravity conditions.

AB - In this study we focused on gravity-sensitive proteins of two human thyroid cancer cell lines (ML-1; RO82-W-1), which were exposed to a 2D clinostat (CLINO), a random positioning machine (RPM) and to normal 1g-conditions. After a three (3d)- or seven-day-culture (7d) on the two devices, we found both cell types growing three-dimensionally within multicellular spheroids (MCS) and also cells remaining adherent (AD) to the culture flask, while 1g-control cultures only formed adherent monolayers, unless the bottom of the culture dish was covered by agarose. In this case, the cytokines IL-6 and IL-8 facilitated the formation of MCS in both cell lines using the liquid-overlay technique at 1g. ML-1 cells grown on the RPM or the CLINO released amounts of IL-6 and MCP-1 into the supernatant, which were significantly elevated as compared to 1g-controls. Release of IL-4, IL-7, IL-8, IL-17, eotaxin-1 and VEGF increased time-dependently, but was not significantly influenced by the gravity conditions. After 3d on the RPM or the CLINO, an accumulation of F-actin around the cellular membrane was detectable in AD cells of both cell lines. IL-6 and IL-8 stimulation of ML-1 cells for 3d and 7d influenced the protein contents of ß1-integrin, talin-1, Ki-67, and beta-actin dose-dependently in adherent cells. The ß1-integrin content was significantly decreased in AD and MCS samples compared with 1g, while talin-1 was higher expressed in MCS than AD populations. The proliferation marker Ki-67 was elevated in AD samples compared with 1g and MCS samples. The ß-actin content of R082-W-1 cells remained unchanged. ML-1 cells exhibited no change in ß-actin in RPM cultures, but a reduction in CLINO samples. Thus, we concluded that simulated microgravity influences the release of cytokines in follicular thyroid cancer cells, and the production of ß1-integrin and talin-1 and predicts an identical effect under real microgravity conditions.

U2 - 10.1371/journal.pone.0135157

DO - 10.1371/journal.pone.0135157

M3 - Journal article

VL - 10

SP - e0135157

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 8

ER -