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Jacob Giehm Mikkelsen

Viral delivery of genome-modifying proteins for cellular reprogramming

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DOI

Following the successful development of virus-based gene vehicles for genetic therapies, exploitation of viruses as carriers of genetic tools for cellular reprogramming and genome editing should be right up the street. However, whereas persistent, potentially life-long gene expression is the main goal of conventional genetic therapies, tools and bits for genome engineering should ideally be short-lived and active only for a limited time. Although viral vector systems have already been adapted for potent genome editing both in vitro and in vivo, regulatable gene expression systems or self-limiting expression circuits need to be implemented limiting exposure of chromatin to genome-modifying enzymes. As an alternative approach, emerging virus-based protein delivery technologies support direct protein delivery, providing a short, robust boost of enzymatic activity in transduced cells. Is this potentially the perfect way of shipping loads of cargo to many recipients and still maintain short-term activity?

OriginalsprogEngelsk
TidsskriftCurrent Opinion in Genetics & Development
Vol/bind52
Sider (fra-til)92-99
Antal sider8
ISSN0959-437X
DOI
StatusUdgivet - okt. 2018

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