Institut for Biomedicin

Jacob Giehm Mikkelsen

Establishment of a pig fibroblast-derived cell line for locus-directed transgene expression in cell cultures and blastocysts

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

We report the establishment of a spontaneously immortalized pig cell line designated Pig Flip-in Visualize (PFV) for locus-directed transgene expression in pig cells and blastocysts. The PFV cell line was isolated from pig ear fibroblasts transfected with a Sleeping Beauty DNA transposon-based docking vector harbouring a selection gene, an eGFP reporter gene, and an Flp recombinase site for locus-directed gene insertion. PFV cells have insertion of a single docking vector with stable eGFP expression and generated phenotypic normal blastocysts with transgene expression after somatic cell nuclear transfer. PFV cells supported Flp mediated cassette exchange for transgene substitution of eGFP with dsRED, and the dsRED transgenic PFV cells generated blastocysts with transgene expression. Hence, the PFV cell line constitutes a valuable pig equivalent to transformed cell lines from other mammalian species suitable for locus-directed transgene expression in cell cultures and, in addition, for transgene analyses in the very early embryonic stages.
OriginalsprogEngelsk
TidsskriftMolecular Biology Reports
Vol/bind38
Nummer1
Sider (fra-til)151-61
Antal sider11
ISSN0301-4851
DOI
StatusUdgivet - 2011

Se relationer på Aarhus Universitet Citationsformater

ID: 51865667