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Istvan Nagy

Fungal Endophyte Colonization Patterns Alter Over Time in the Novel Association Between Lolium perenne and Epichloë Endophyte AR37

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Standard

Fungal Endophyte Colonization Patterns Alter Over Time in the Novel Association Between Lolium perenne and Epichloë Endophyte AR37. / Forte, Flavia Pilar; Schmid, Jan; Dijkwel, Paul P.; Nagy, Istvan; Hume, David E.; Johnson, Richard D.; Simpson, Wayne R.; Monk, Shaun M.; Zhang, Ningxin; Sehrish, Tina; Asp, Torben.

I: Frontiers in Plant Science, Bind 11, 570026, 10.2020.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Forte, FP, Schmid, J, Dijkwel, PP, Nagy, I, Hume, DE, Johnson, RD, Simpson, WR, Monk, SM, Zhang, N, Sehrish, T & Asp, T 2020, 'Fungal Endophyte Colonization Patterns Alter Over Time in the Novel Association Between Lolium perenne and Epichloë Endophyte AR37', Frontiers in Plant Science, bind 11, 570026. https://doi.org/10.3389/fpls.2020.570026

APA

Forte, F. P., Schmid, J., Dijkwel, P. P., Nagy, I., Hume, D. E., Johnson, R. D., Simpson, W. R., Monk, S. M., Zhang, N., Sehrish, T., & Asp, T. (2020). Fungal Endophyte Colonization Patterns Alter Over Time in the Novel Association Between Lolium perenne and Epichloë Endophyte AR37. Frontiers in Plant Science, 11, [570026]. https://doi.org/10.3389/fpls.2020.570026

CBE

Forte FP, Schmid J, Dijkwel PP, Nagy I, Hume DE, Johnson RD, Simpson WR, Monk SM, Zhang N, Sehrish T, Asp T. 2020. Fungal Endophyte Colonization Patterns Alter Over Time in the Novel Association Between Lolium perenne and Epichloë Endophyte AR37. Frontiers in Plant Science. 11:Article 570026. https://doi.org/10.3389/fpls.2020.570026

MLA

Vancouver

Author

Forte, Flavia Pilar ; Schmid, Jan ; Dijkwel, Paul P. ; Nagy, Istvan ; Hume, David E. ; Johnson, Richard D. ; Simpson, Wayne R. ; Monk, Shaun M. ; Zhang, Ningxin ; Sehrish, Tina ; Asp, Torben. / Fungal Endophyte Colonization Patterns Alter Over Time in the Novel Association Between Lolium perenne and Epichloë Endophyte AR37. I: Frontiers in Plant Science. 2020 ; Bind 11.

Bibtex

@article{d6d2b03241fe437e904fa8bfa7f4df27,
title = "Fungal Endophyte Colonization Patterns Alter Over Time in the Novel Association Between Lolium perenne and Epichlo{\"e} Endophyte AR37",
abstract = "Infection of the pasture grass Lolium perenne with the seed-transmitted fungal endophyte Epichlo{\"e} festucae enhances its resilience to biotic and abiotic stress. Agricultural benefits of endophyte infection can be increased by generating novel symbiotic associations through inoculating L. perenne with selected Epichlo{\"e} strains. Natural symbioses have coevolved over long periods. Thus, artificial symbioses will probably not have static properties, but symbionts will coadapt over time improving the fitness of the association. Here we report for the first time on temporal changes in a novel association of Epichlo{\"e} strain AR37 and the L. perenne cultivar Grasslands Samson. Over nine generations, a seed maintenance program had increased the endophyte seed transmission rates to > 95% (from an initial 76%). We observed an approximately fivefold decline in endophyte biomass concentration in vegetative tissues over time (between generations 2 and 9). This indicates strong selection pressure toward reducing endophyte-related fitness costs by reducing endophyte biomass, without compromising the frequency of endophyte transmission to seed. We observed no obvious changes in tillering and only minor transcriptomic changes in infected plants over time. Functional analysis of 40 plant genes, showing continuously decreasing expression over time, suggests that adaptation of host metabolism and defense mechanisms are important for increasing the fitness of this association, and possibly fitness of such symbioses in general. Our results indicate that fitness of novel associations is likely to improve over time and that monitoring changes in novel associations can assist in identifying key features of endophyte-mediated enhancement of host fitness.",
keywords = "artificial association, coadaptation, Epichlo{\"e} endophyte AR37, fungal colonization, Lolium perenne, seed maintenance program",
author = "Forte, {Flavia Pilar} and Jan Schmid and Dijkwel, {Paul P.} and Istvan Nagy and Hume, {David E.} and Johnson, {Richard D.} and Simpson, {Wayne R.} and Monk, {Shaun M.} and Ningxin Zhang and Tina Sehrish and Torben Asp",
year = "2020",
month = oct,
doi = "10.3389/fpls.2020.570026",
language = "English",
volume = "11",
journal = "Frontiers in Plant Science",
issn = "1664-462X",
publisher = "Frontiers Media S.A",

}

RIS

TY - JOUR

T1 - Fungal Endophyte Colonization Patterns Alter Over Time in the Novel Association Between Lolium perenne and Epichloë Endophyte AR37

AU - Forte, Flavia Pilar

AU - Schmid, Jan

AU - Dijkwel, Paul P.

AU - Nagy, Istvan

AU - Hume, David E.

AU - Johnson, Richard D.

AU - Simpson, Wayne R.

AU - Monk, Shaun M.

AU - Zhang, Ningxin

AU - Sehrish, Tina

AU - Asp, Torben

PY - 2020/10

Y1 - 2020/10

N2 - Infection of the pasture grass Lolium perenne with the seed-transmitted fungal endophyte Epichloë festucae enhances its resilience to biotic and abiotic stress. Agricultural benefits of endophyte infection can be increased by generating novel symbiotic associations through inoculating L. perenne with selected Epichloë strains. Natural symbioses have coevolved over long periods. Thus, artificial symbioses will probably not have static properties, but symbionts will coadapt over time improving the fitness of the association. Here we report for the first time on temporal changes in a novel association of Epichloë strain AR37 and the L. perenne cultivar Grasslands Samson. Over nine generations, a seed maintenance program had increased the endophyte seed transmission rates to > 95% (from an initial 76%). We observed an approximately fivefold decline in endophyte biomass concentration in vegetative tissues over time (between generations 2 and 9). This indicates strong selection pressure toward reducing endophyte-related fitness costs by reducing endophyte biomass, without compromising the frequency of endophyte transmission to seed. We observed no obvious changes in tillering and only minor transcriptomic changes in infected plants over time. Functional analysis of 40 plant genes, showing continuously decreasing expression over time, suggests that adaptation of host metabolism and defense mechanisms are important for increasing the fitness of this association, and possibly fitness of such symbioses in general. Our results indicate that fitness of novel associations is likely to improve over time and that monitoring changes in novel associations can assist in identifying key features of endophyte-mediated enhancement of host fitness.

AB - Infection of the pasture grass Lolium perenne with the seed-transmitted fungal endophyte Epichloë festucae enhances its resilience to biotic and abiotic stress. Agricultural benefits of endophyte infection can be increased by generating novel symbiotic associations through inoculating L. perenne with selected Epichloë strains. Natural symbioses have coevolved over long periods. Thus, artificial symbioses will probably not have static properties, but symbionts will coadapt over time improving the fitness of the association. Here we report for the first time on temporal changes in a novel association of Epichloë strain AR37 and the L. perenne cultivar Grasslands Samson. Over nine generations, a seed maintenance program had increased the endophyte seed transmission rates to > 95% (from an initial 76%). We observed an approximately fivefold decline in endophyte biomass concentration in vegetative tissues over time (between generations 2 and 9). This indicates strong selection pressure toward reducing endophyte-related fitness costs by reducing endophyte biomass, without compromising the frequency of endophyte transmission to seed. We observed no obvious changes in tillering and only minor transcriptomic changes in infected plants over time. Functional analysis of 40 plant genes, showing continuously decreasing expression over time, suggests that adaptation of host metabolism and defense mechanisms are important for increasing the fitness of this association, and possibly fitness of such symbioses in general. Our results indicate that fitness of novel associations is likely to improve over time and that monitoring changes in novel associations can assist in identifying key features of endophyte-mediated enhancement of host fitness.

KW - artificial association

KW - coadaptation

KW - Epichloë endophyte AR37

KW - fungal colonization

KW - Lolium perenne

KW - seed maintenance program

UR - http://www.scopus.com/inward/record.url?scp=85096017498&partnerID=8YFLogxK

U2 - 10.3389/fpls.2020.570026

DO - 10.3389/fpls.2020.570026

M3 - Journal article

C2 - 33193501

AN - SCOPUS:85096017498

VL - 11

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

M1 - 570026

ER -