Hanne Primdahl

Immunohistochemical study of the expression of cell cycle regulating proteins at different stages of bladder cancer

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Immunohistochemical study of the expression of cell cycle regulating proteins at different stages of bladder cancer. / Primdahl, Hanne; von der Maase, Hans; Sørensen, Flemming Brandt; Wolf, Hans; Ørntoft, Torben F.

I: Journal of Cancer Research and Clinical Oncology, Bind 128, Nr. 6, 06.2002, s. 295-301.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Primdahl, Hanne ; von der Maase, Hans ; Sørensen, Flemming Brandt ; Wolf, Hans ; Ørntoft, Torben F. / Immunohistochemical study of the expression of cell cycle regulating proteins at different stages of bladder cancer. I: Journal of Cancer Research and Clinical Oncology. 2002 ; Bind 128, Nr. 6. s. 295-301.

Bibtex

@article{984c62428e214849aa34629798e16462,
title = "Immunohistochemical study of the expression of cell cycle regulating proteins at different stages of bladder cancer",
abstract = "PURPOSE: The cell cycle is known to be deregulated in cancer. We therefore analyzed the expression of the cell cycle related proteins p21, p27, p16, Rb, and L-myc by immunohistochemical staining of bladder tumors.METHODS: The tissue material consisted of bladder tumors from three groups of patients; group 1, 23 patients with recurrent stage Ta (non-invasive) tumors; group 2, 22 patients presenting at their first admission with T2-4 (muscle invasive) tumors; group 3, 24 patients who experienced disease progression from Ta or T1 (invasive in connective tissue) to a higher stage. By immunohistochemical staining the protein expression was compared to allelic deletions of the corresponding genes. The allelic deletions were detected by PCR-based microsatellite analyses.RESULTS: We detected a significant reduction in the expression levels of the cell cycle related proteins p21(waf1) ( P=0.002), p27(kip1) ( P=0.03), Rb ( P=0.00002), and L-myc ( P=0.00000007) in muscle invasive tumors compared to noninvasive tumors. Tumors presenting as muscle invasive at first diagnosis had significantly lower levels of p16/CDKN2A ( P=0.01) when compared to muscle invasive tumors that followed Ta or T1 precursor lesions. We found no general correlation between allelic deletion of a gene and its immunohistochemical protein expression, indicating that the remaining allele may be capable of encoding a normal or even increased protein level.CONCLUSIONS: Our results support the hypothesis that bladder tumors with invasion at first diagnosis differ from those in which invasion follow superficial tumors. This difference is reflected as a different level in cell cycle related protein expression. The data also indicate that allelic deletions of cell cycle related genes do not correlate with an altered level of protein expression.",
keywords = "Cell Cycle Proteins, Cyclin-Dependent Kinase Inhibitor p16, Gene Expression Regulation, Neoplastic, Humans, Immunohistochemistry, Kidney Neoplasms, Loss of Heterozygosity, Microsatellite Repeats, Neoplasm Invasiveness, Neoplasm Staging, Polymerase Chain Reaction, Recurrence, Retrospective Studies",
author = "Hanne Primdahl and {von der Maase}, Hans and S{\o}rensen, {Flemming Brandt} and Hans Wolf and {\O}rntoft, {Torben F}",
year = "2002",
month = jun,
doi = "10.1007/s00432-002-0344-3",
language = "English",
volume = "128",
pages = "295--301",
journal = "Journal of Cancer Research and Clinical Oncology",
issn = "0171-5216",
publisher = "Springer",
number = "6",

}

RIS

TY - JOUR

T1 - Immunohistochemical study of the expression of cell cycle regulating proteins at different stages of bladder cancer

AU - Primdahl, Hanne

AU - von der Maase, Hans

AU - Sørensen, Flemming Brandt

AU - Wolf, Hans

AU - Ørntoft, Torben F

PY - 2002/6

Y1 - 2002/6

N2 - PURPOSE: The cell cycle is known to be deregulated in cancer. We therefore analyzed the expression of the cell cycle related proteins p21, p27, p16, Rb, and L-myc by immunohistochemical staining of bladder tumors.METHODS: The tissue material consisted of bladder tumors from three groups of patients; group 1, 23 patients with recurrent stage Ta (non-invasive) tumors; group 2, 22 patients presenting at their first admission with T2-4 (muscle invasive) tumors; group 3, 24 patients who experienced disease progression from Ta or T1 (invasive in connective tissue) to a higher stage. By immunohistochemical staining the protein expression was compared to allelic deletions of the corresponding genes. The allelic deletions were detected by PCR-based microsatellite analyses.RESULTS: We detected a significant reduction in the expression levels of the cell cycle related proteins p21(waf1) ( P=0.002), p27(kip1) ( P=0.03), Rb ( P=0.00002), and L-myc ( P=0.00000007) in muscle invasive tumors compared to noninvasive tumors. Tumors presenting as muscle invasive at first diagnosis had significantly lower levels of p16/CDKN2A ( P=0.01) when compared to muscle invasive tumors that followed Ta or T1 precursor lesions. We found no general correlation between allelic deletion of a gene and its immunohistochemical protein expression, indicating that the remaining allele may be capable of encoding a normal or even increased protein level.CONCLUSIONS: Our results support the hypothesis that bladder tumors with invasion at first diagnosis differ from those in which invasion follow superficial tumors. This difference is reflected as a different level in cell cycle related protein expression. The data also indicate that allelic deletions of cell cycle related genes do not correlate with an altered level of protein expression.

AB - PURPOSE: The cell cycle is known to be deregulated in cancer. We therefore analyzed the expression of the cell cycle related proteins p21, p27, p16, Rb, and L-myc by immunohistochemical staining of bladder tumors.METHODS: The tissue material consisted of bladder tumors from three groups of patients; group 1, 23 patients with recurrent stage Ta (non-invasive) tumors; group 2, 22 patients presenting at their first admission with T2-4 (muscle invasive) tumors; group 3, 24 patients who experienced disease progression from Ta or T1 (invasive in connective tissue) to a higher stage. By immunohistochemical staining the protein expression was compared to allelic deletions of the corresponding genes. The allelic deletions were detected by PCR-based microsatellite analyses.RESULTS: We detected a significant reduction in the expression levels of the cell cycle related proteins p21(waf1) ( P=0.002), p27(kip1) ( P=0.03), Rb ( P=0.00002), and L-myc ( P=0.00000007) in muscle invasive tumors compared to noninvasive tumors. Tumors presenting as muscle invasive at first diagnosis had significantly lower levels of p16/CDKN2A ( P=0.01) when compared to muscle invasive tumors that followed Ta or T1 precursor lesions. We found no general correlation between allelic deletion of a gene and its immunohistochemical protein expression, indicating that the remaining allele may be capable of encoding a normal or even increased protein level.CONCLUSIONS: Our results support the hypothesis that bladder tumors with invasion at first diagnosis differ from those in which invasion follow superficial tumors. This difference is reflected as a different level in cell cycle related protein expression. The data also indicate that allelic deletions of cell cycle related genes do not correlate with an altered level of protein expression.

KW - Cell Cycle Proteins

KW - Cyclin-Dependent Kinase Inhibitor p16

KW - Gene Expression Regulation, Neoplastic

KW - Humans

KW - Immunohistochemistry

KW - Kidney Neoplasms

KW - Loss of Heterozygosity

KW - Microsatellite Repeats

KW - Neoplasm Invasiveness

KW - Neoplasm Staging

KW - Polymerase Chain Reaction

KW - Recurrence

KW - Retrospective Studies

U2 - 10.1007/s00432-002-0344-3

DO - 10.1007/s00432-002-0344-3

M3 - Journal article

C2 - 12073046

VL - 128

SP - 295

EP - 301

JO - Journal of Cancer Research and Clinical Oncology

JF - Journal of Cancer Research and Clinical Oncology

SN - 0171-5216

IS - 6

ER -