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Hanne Johansen

Determination of 15N abundance in nanogram pools of NO3 - and NO2 - by denitrification bioassay and mass spectrometry

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Determination of 15N abundance in nanogram pools of NO3 - and NO2 - by denitrification bioassay and mass spectrometry. / Højberg, Ole; Johansen, H. S.; Sorensen, J.

I: Applied and Environmental Microbiology, Bind 60, Nr. 7, 01.01.1994, s. 2467-2472.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Højberg, Ole ; Johansen, H. S. ; Sorensen, J. / Determination of 15N abundance in nanogram pools of NO3 - and NO2 - by denitrification bioassay and mass spectrometry. I: Applied and Environmental Microbiology. 1994 ; Bind 60, Nr. 7. s. 2467-2472.

Bibtex

@article{78ea495ecc904b60b5dd84f6148784ff,
title = "Determination of 15N abundance in nanogram pools of NO3 - and NO2 - by denitrification bioassay and mass spectrometry",
abstract = "Suspensions of two strains of Pseudomonas aeruginosa (ON12 and ON12-1) were used to reduce NO3 - and NO2 -, respectively, to N2O. The evolved N2O was quantified by gas chromatography with electron capture detection, and the 15N abundance was determined by mass spectrometry with a special inlet system and triple-collector detection. Sample gas containing unknown N2O pools as small as 0.5 ng of N was analyzed by use of a spike technique, in which a reference gas of N2O of natural 15N abundance was added to obtain enough total N for the mass spectrometer. In NO3 - or NO2 - pools, the 15N abundance could be determined in samples as small as approximately 3.5 ng of N. No cross-contamination took place between the NO3 - and NO2 - pools. The excellent separation of NO3 - and NO2 - pools, small sample size required, and low contamination risk during N2O analysis offer great advantages in isotope studies of inorganic N transformations by, e.g., nitrifying or denitrifying bacteria in the environment.",
author = "Ole H{\o}jberg and Johansen, {H. S.} and J. Sorensen",
year = "1994",
month = jan,
day = "1",
language = "English",
volume = "60",
pages = "2467--2472",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "7",

}

RIS

TY - JOUR

T1 - Determination of 15N abundance in nanogram pools of NO3 - and NO2 - by denitrification bioassay and mass spectrometry

AU - Højberg, Ole

AU - Johansen, H. S.

AU - Sorensen, J.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Suspensions of two strains of Pseudomonas aeruginosa (ON12 and ON12-1) were used to reduce NO3 - and NO2 -, respectively, to N2O. The evolved N2O was quantified by gas chromatography with electron capture detection, and the 15N abundance was determined by mass spectrometry with a special inlet system and triple-collector detection. Sample gas containing unknown N2O pools as small as 0.5 ng of N was analyzed by use of a spike technique, in which a reference gas of N2O of natural 15N abundance was added to obtain enough total N for the mass spectrometer. In NO3 - or NO2 - pools, the 15N abundance could be determined in samples as small as approximately 3.5 ng of N. No cross-contamination took place between the NO3 - and NO2 - pools. The excellent separation of NO3 - and NO2 - pools, small sample size required, and low contamination risk during N2O analysis offer great advantages in isotope studies of inorganic N transformations by, e.g., nitrifying or denitrifying bacteria in the environment.

AB - Suspensions of two strains of Pseudomonas aeruginosa (ON12 and ON12-1) were used to reduce NO3 - and NO2 -, respectively, to N2O. The evolved N2O was quantified by gas chromatography with electron capture detection, and the 15N abundance was determined by mass spectrometry with a special inlet system and triple-collector detection. Sample gas containing unknown N2O pools as small as 0.5 ng of N was analyzed by use of a spike technique, in which a reference gas of N2O of natural 15N abundance was added to obtain enough total N for the mass spectrometer. In NO3 - or NO2 - pools, the 15N abundance could be determined in samples as small as approximately 3.5 ng of N. No cross-contamination took place between the NO3 - and NO2 - pools. The excellent separation of NO3 - and NO2 - pools, small sample size required, and low contamination risk during N2O analysis offer great advantages in isotope studies of inorganic N transformations by, e.g., nitrifying or denitrifying bacteria in the environment.

M3 - Journal article

AN - SCOPUS:0028181321

VL - 60

SP - 2467

EP - 2472

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 7

ER -