TY - JOUR

T1 - Membrane composition of polymer-lipid hybrid vesicles

AU - Brodszkij, Edit

AU - Westensee, Isabella Nymann

AU - Holleufer, Steffen Friis

AU - Ade, Carina

AU - Andres, Paula De Dios

AU - Pedersen, Jan Skov

AU - Städler, Brigitte

N1 - Publisher Copyright: © 2022 The Author(s)

PY - 2022/12

Y1 - 2022/12

N2 - Hybrid vesicles (HVs) assembled from phospholipids and amphiphilic block copolymers (BCPs) are a more recent alternative to liposomes and polymersomes. We aim to change the properties of the HV membranes by varying the chemical composition of the hydrophobic block in the BCPs that have poly(carboxyethyl acrylate) (PCEA) as the hydrophilic part. To this end, statistical copolymers of cholesteryl methacrylate and either butyl methacrylate (BuMA) or 2-hydroxyethyl methacrylate (HEMA) as well as the corresponding homopolymers were synthesized and used as macroinitiator for the extension with PCEA. All the BCPs allowed for the assembly of small and giant HVs with soybean L-α-phosphatidylcholine. The extend of the co-extisting micellar populations varied as shown by transmission electron microscopy and small-angle X-ray spectroscopy. Although the membrane packings derived from spectra when using Laurdan as an environmentally sensitive fluorescent probe were comparable between the different HVs, their permeability towards 5(6)-carboxy-X-rhodamine or carboxyfluorescein depended on the membrane composition, i.e., HEMA-containing membranes had higher permeability than membranes containing the other tested BCPs for small and giant HVs. Further, membranes with BuMA offered the most suitable environment for the association with β-galactosidase illustrated by the efficient substrate conversion. Taken together, the hydrophobic block is a relevant mean to control the morphologies and membrane properties of HVs.

AB - Hybrid vesicles (HVs) assembled from phospholipids and amphiphilic block copolymers (BCPs) are a more recent alternative to liposomes and polymersomes. We aim to change the properties of the HV membranes by varying the chemical composition of the hydrophobic block in the BCPs that have poly(carboxyethyl acrylate) (PCEA) as the hydrophilic part. To this end, statistical copolymers of cholesteryl methacrylate and either butyl methacrylate (BuMA) or 2-hydroxyethyl methacrylate (HEMA) as well as the corresponding homopolymers were synthesized and used as macroinitiator for the extension with PCEA. All the BCPs allowed for the assembly of small and giant HVs with soybean L-α-phosphatidylcholine. The extend of the co-extisting micellar populations varied as shown by transmission electron microscopy and small-angle X-ray spectroscopy. Although the membrane packings derived from spectra when using Laurdan as an environmentally sensitive fluorescent probe were comparable between the different HVs, their permeability towards 5(6)-carboxy-X-rhodamine or carboxyfluorescein depended on the membrane composition, i.e., HEMA-containing membranes had higher permeability than membranes containing the other tested BCPs for small and giant HVs. Further, membranes with BuMA offered the most suitable environment for the association with β-galactosidase illustrated by the efficient substrate conversion. Taken together, the hydrophobic block is a relevant mean to control the morphologies and membrane properties of HVs.

KW - ARTIFICIAL CELLS

KW - BLOCK-COPOLYMERS

KW - GENERALIZED POLARIZATION

KW - LIPOSOMES

KW - MICELLES

KW - NANOSCALE

KW - NANOVESICLES

KW - PHOSPHOLIPID-VESICLES

KW - PLANT STEROLS

KW - X-RAY-SCATTERING

UR - http://www.scopus.com/inward/record.url?scp=85133408817&partnerID=8YFLogxK

U2 - 10.1016/j.apmt.2022.101549

DO - 10.1016/j.apmt.2022.101549

M3 - Journal article

AN - SCOPUS:85133408817

VL - 29

JO - Applied Materials Today

JF - Applied Materials Today

SN - 2352-9407

M1 - 101549

ER -