Institut for Biomedicin

Claudia Cecchi Kaadt

An homologous model of gene therapy by in vivo administration of a plasmid containing the mouse growth hormone gene in immunocompetent dwarf mice

Publikation: KonferencebidragPosterForskningpeer review

DOI

An alternative method for growth hormone deficiency (GHD )treatment has been developed using gene therapy associated to electroporation. Sustained circulatory levels of hGH and a highly significant weight increase were obtained in immunodeficient dwarf (lit/scid) mice. This new study aimed at setting up an homologous model of gene therapy based on electrotransfer of genomic mouse growth hormone (gmGH) in the muscle of im-munocompetent dwarf (lit/lit) mice. The plasmid pUC-UBI-gmGH was first transfected into HEK 293 cells and an in vitro expression up to 74.0 ng mGH/106 cells/day, compared to 6.0 ng mGH/106 cells/day for the negative control, was obtained. As apositive control for in vivo expression, this plasmid was administered as naked DNA via hydrodynamic injection into C57BL6 normal mice, and the weight increase of treated mice after 21days was 60.10%–11.02% compared to-2.05%–2.32% for thecontrol group (injected with Ringer’s solution). The plasmid was then administered in a protocol of gene-electrotransfer, applying eight 150 V/cm pulses of 20 ms, for a body weight gain assay into lit/lit mice. The weight increase after 15 days was 13.48%–3.53%for the gmGH-treated group versus 5.66%–2.51% for the control group (injected with saline) and 13.10%–7.54% for the group injected with the same plasmid encoding the ghGH gene, both groups also followed by electroporation. This previous results can be considered very promising as a pre-clinical study in a gene therapy protocol for patients suffering for GHD.

OriginalsprogEngelsk
Udgivelsesår2012
DOI
StatusUdgivet - 2012
BegivenhedAnnual Congress of the European Society of Gene and Cell Therapy - Palais des Congrès de Versailles, Versailles, Frankrig
Varighed: 26 okt. 201229 okt. 2012

Konference

KonferenceAnnual Congress of the European Society of Gene and Cell Therapy
LokationPalais des Congrès de Versailles
LandFrankrig
ByVersailles
Periode26/10/201229/10/2012

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