Institut for Biomedicin

Christian Bjerggaard Vægter

Peripheral Nerve Regeneration Is Independent From Schwann Cell p75NTR Expression

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Peripheral Nerve Regeneration Is Independent From Schwann Cell p75NTR Expression. / Gonçalves, Nádia P; Mohseni, Simin; El Soury, Marwa; Ulrichsen, Maj; Richner, Mette; Xiao, Junhua; Wood, Rhiannon J; Andersen, Olav M; Coulson, Elizabeth J; Raimondo, Stefania; Murray, Simon S; Vægter, Christian B.

I: Frontiers in Cellular Neuroscience, Bind 13, 235, 2019.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Gonçalves, NP, Mohseni, S, El Soury, M, Ulrichsen, M, Richner, M, Xiao, J, Wood, RJ, Andersen, OM, Coulson, EJ, Raimondo, S, Murray, SS & Vægter, CB 2019, 'Peripheral Nerve Regeneration Is Independent From Schwann Cell p75NTR Expression', Frontiers in Cellular Neuroscience, bind 13, 235. https://doi.org/10.3389/fncel.2019.00235

APA

CBE

Gonçalves NP, Mohseni S, El Soury M, Ulrichsen M, Richner M, Xiao J, Wood RJ, Andersen OM, Coulson EJ, Raimondo S, Murray SS, Vægter CB. 2019. Peripheral Nerve Regeneration Is Independent From Schwann Cell p75NTR Expression. Frontiers in Cellular Neuroscience. 13. https://doi.org/10.3389/fncel.2019.00235

MLA

Vancouver

Author

Gonçalves, Nádia P ; Mohseni, Simin ; El Soury, Marwa ; Ulrichsen, Maj ; Richner, Mette ; Xiao, Junhua ; Wood, Rhiannon J ; Andersen, Olav M ; Coulson, Elizabeth J ; Raimondo, Stefania ; Murray, Simon S ; Vægter, Christian B. / Peripheral Nerve Regeneration Is Independent From Schwann Cell p75NTR Expression. I: Frontiers in Cellular Neuroscience. 2019 ; Bind 13.

Bibtex

@article{6d13f332ca424babae7eb1d246f3e3fb,
title = "Peripheral Nerve Regeneration Is Independent From Schwann Cell p75NTR Expression",
abstract = "Schwann cell reprogramming and differentiation are crucial prerequisites for neuronal regeneration and re-myelination to occur following injury to peripheral nerves. The neurotrophin receptor p75NTR has been identified as a positive modulator for Schwann cell myelination during development and implicated in promoting nerve regeneration after injury. However, most studies base this conclusion on results obtained from complete p75NTR knockout mouse models and cannot dissect the specific role of p75NTR expressed by Schwann cells. In this present study, a conditional knockout model selectively deleting p75NTR expression in Schwann cells was generated, where p75NTR expression is replaced with that of an mCherry reporter. Silencing of Schwann cell p75NTR expression was confirmed in the sciatic nerve in vivo and in vitro, without altering axonal expression of p75NTR. No difference in sciatic nerve myelination during development or following sciatic nerve crush injury was observed, as determined by quantification of both myelinated and unmyelinated nerve fiber densities, myelinated axonal diameter and myelin thickness. However, the absence of Schwann cell p75NTR reduced motor nerve conduction velocity after crush injury. Our data indicate that the absence of Schwann cell p75NTR expression in vivo is not critical for axonal regrowth or remyelination following sciatic nerve crush injury, but does play a key role in functional recovery. Overall, this represents the first step in redefining the role of p75NTR in the peripheral nervous system, suggesting that the Schwann cell-axon unit functions as a syncytium, with the previous published involvement of p75NTR in remyelination most likely depending on axonal/neuronal p75NTR and/or mutual glial-axonal interactions.",
keywords = "CONDUCTION-VELOCITY, DELETION, GROWTH-FACTOR, LOCALIZATION, MYELIN FORMATION, NEUROTROPHIN RECEPTOR P75(NTR), NUMBER, P75, SCIATIC-NERVE, Schwann cells, TARGETED MUTATION, myelination, nerve injury, p75(NTR), regeneration",
author = "Gon{\cc}alves, {N{\'a}dia P} and Simin Mohseni and {El Soury}, Marwa and Maj Ulrichsen and Mette Richner and Junhua Xiao and Wood, {Rhiannon J} and Andersen, {Olav M} and Coulson, {Elizabeth J} and Stefania Raimondo and Murray, {Simon S} and V{\ae}gter, {Christian B}",
year = "2019",
doi = "10.3389/fncel.2019.00235",
language = "English",
volume = "13",
journal = "Frontiers in Cellular Neuroscience",
issn = "1662-5102",
publisher = "Frontiers Media S.A",

}

RIS

TY - JOUR

T1 - Peripheral Nerve Regeneration Is Independent From Schwann Cell p75NTR Expression

AU - Gonçalves, Nádia P

AU - Mohseni, Simin

AU - El Soury, Marwa

AU - Ulrichsen, Maj

AU - Richner, Mette

AU - Xiao, Junhua

AU - Wood, Rhiannon J

AU - Andersen, Olav M

AU - Coulson, Elizabeth J

AU - Raimondo, Stefania

AU - Murray, Simon S

AU - Vægter, Christian B

PY - 2019

Y1 - 2019

N2 - Schwann cell reprogramming and differentiation are crucial prerequisites for neuronal regeneration and re-myelination to occur following injury to peripheral nerves. The neurotrophin receptor p75NTR has been identified as a positive modulator for Schwann cell myelination during development and implicated in promoting nerve regeneration after injury. However, most studies base this conclusion on results obtained from complete p75NTR knockout mouse models and cannot dissect the specific role of p75NTR expressed by Schwann cells. In this present study, a conditional knockout model selectively deleting p75NTR expression in Schwann cells was generated, where p75NTR expression is replaced with that of an mCherry reporter. Silencing of Schwann cell p75NTR expression was confirmed in the sciatic nerve in vivo and in vitro, without altering axonal expression of p75NTR. No difference in sciatic nerve myelination during development or following sciatic nerve crush injury was observed, as determined by quantification of both myelinated and unmyelinated nerve fiber densities, myelinated axonal diameter and myelin thickness. However, the absence of Schwann cell p75NTR reduced motor nerve conduction velocity after crush injury. Our data indicate that the absence of Schwann cell p75NTR expression in vivo is not critical for axonal regrowth or remyelination following sciatic nerve crush injury, but does play a key role in functional recovery. Overall, this represents the first step in redefining the role of p75NTR in the peripheral nervous system, suggesting that the Schwann cell-axon unit functions as a syncytium, with the previous published involvement of p75NTR in remyelination most likely depending on axonal/neuronal p75NTR and/or mutual glial-axonal interactions.

AB - Schwann cell reprogramming and differentiation are crucial prerequisites for neuronal regeneration and re-myelination to occur following injury to peripheral nerves. The neurotrophin receptor p75NTR has been identified as a positive modulator for Schwann cell myelination during development and implicated in promoting nerve regeneration after injury. However, most studies base this conclusion on results obtained from complete p75NTR knockout mouse models and cannot dissect the specific role of p75NTR expressed by Schwann cells. In this present study, a conditional knockout model selectively deleting p75NTR expression in Schwann cells was generated, where p75NTR expression is replaced with that of an mCherry reporter. Silencing of Schwann cell p75NTR expression was confirmed in the sciatic nerve in vivo and in vitro, without altering axonal expression of p75NTR. No difference in sciatic nerve myelination during development or following sciatic nerve crush injury was observed, as determined by quantification of both myelinated and unmyelinated nerve fiber densities, myelinated axonal diameter and myelin thickness. However, the absence of Schwann cell p75NTR reduced motor nerve conduction velocity after crush injury. Our data indicate that the absence of Schwann cell p75NTR expression in vivo is not critical for axonal regrowth or remyelination following sciatic nerve crush injury, but does play a key role in functional recovery. Overall, this represents the first step in redefining the role of p75NTR in the peripheral nervous system, suggesting that the Schwann cell-axon unit functions as a syncytium, with the previous published involvement of p75NTR in remyelination most likely depending on axonal/neuronal p75NTR and/or mutual glial-axonal interactions.

KW - CONDUCTION-VELOCITY

KW - DELETION

KW - GROWTH-FACTOR

KW - LOCALIZATION

KW - MYELIN FORMATION

KW - NEUROTROPHIN RECEPTOR P75(NTR)

KW - NUMBER

KW - P75

KW - SCIATIC-NERVE

KW - Schwann cells

KW - TARGETED MUTATION

KW - myelination

KW - nerve injury

KW - p75(NTR)

KW - regeneration

UR - http://www.scopus.com/inward/record.url?scp=85068446001&partnerID=8YFLogxK

U2 - 10.3389/fncel.2019.00235

DO - 10.3389/fncel.2019.00235

M3 - Journal article

VL - 13

JO - Frontiers in Cellular Neuroscience

JF - Frontiers in Cellular Neuroscience

SN - 1662-5102

M1 - 235

ER -