Christian Aalkjær

The human thoracic duct is functionally innervated by adrenergic nerves

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Lymphatic vessels from animals have been shown to be innervated. While morphological studies have confirmed human lymphatic vessels are innervated, functional studies supporting this are lacking. The current study demonstrates a functional innervation of the human thoracic duct that is predominantly adrenergic. The thoracic duct (TD), harvested from 51 patients undergoing esophageal and cardia cancer surgery, were either fixed for structural investigations or maintained in vitro for functional assessment of innervation by isometric force measurements and electrical field stimulation (EFS). Electron microscopy and immunohistochemistry suggested scarce diffuse distribution of nerves in the entire vessel wall but nerve-mediated contractions could be induced with EFS and were sensitive to the muscarinic receptor blocker atropine and the α-adrenoceptor blocker phentolamine. The combination of phentolamine and atropine resulted in a near complete abolishment of the EFS-induced contractions. The presence of sympathetic nerves was further confirmed by contractions induced by the sympathomimetic and catecholamine-releasing agent tyramine. Reactivity to the neurotransmitters norepinephrine, substance P, neuropeptide Y, acetylcholine and methacholine was demonstrated by exogenous application to human TD ring segments. Norepinephrine provided the most consistent responses whereas responses to the other agonists varied. We conclude that the human TD is functionally innervated with both cholinergic and adrenergic components with the latter of the two dominating.
OriginalsprogEngelsk
TidsskriftA J P: Heart and Circulatory Physiology (Online)
Vol/bind306
Nummer2
Sider (fra-til)H206-13
Antal sider8
ISSN1522-1539
DOI
StatusUdgivet - 8 nov. 2013

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