Institut for Biomedicin

Christian Aalkjær

Extracellular Calcium-Dependent Modulation of Endothelium Relaxation in Rat Mesenteric Small Artery: The Role of Potassium Signaling

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DOI

  • Lise Hangaard, Aarhus, Universitet, Institut for Biomedicin - Vest, Danmark
  • Peter B Jessen, Aarhus, Universitet, Institut for Biomedicin - Vest, Danmark
  • Dmitrii Kamaev
  • ,
  • Christian Aalkjaer
  • Vladimir V Matchkov

The nature of NO- and COX-independent endothelial hyperpolarization (EDH) is not fully understood but activation of small- and intermittent-conductance Ca(2+)-activated K(+) channels (SKCa and IKCa) is important. Previous studies have suggested that the significance of IKCa depends on [Ca(2+)]out. Also it has been suggested that K(+) is important through localized [K(+)]out signaling causing activation of the Na(+),K(+)-ATPase and inward-rectifying K(+) channels (Kir). Here we tested the hypothesis that the modulating effect of [Ca(2+)]out on the EDH-like response depends on [K(+)]out. We addressed this possibility using isometric myography of rat mesenteric small arteries. When [K(+)]out was 4.2 mM, relaxation to acetylcholine (ACh) was stronger at 2.5 mM [Ca(2+)]out than at 1 mM [Ca(2+)]out. Inhibition of IKCa with TRAM34 suppressed the relaxations but did not change the relation between the relaxations at the low and high [Ca(2+)]out. This [Ca(2+)]out-dependence disappeared at 5.9 mM [K(+)]out and in the presence of ouabain or BaCl2. Our results suggest that IKCa are involved in the localized [K(+)]out signaling which acts through the Na(+),K(+)-ATPase and Kir channels and that the significance of this endothelium-dependent pathway is modulated by [Ca(2+)]out.

OriginalsprogEngelsk
TidsskriftBioMed Research International
Vol/bind2015
Sider (fra-til)758346
ISSN2314-6133
DOI
StatusUdgivet - okt. 2015

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