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Charlotte Rohde Knudsen

One-step purification of E. coli elongation factor Tu.

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Standard

One-step purification of E. coli elongation factor Tu. / Knudsen, Charlotte Rohde; Clark, Brian F. C.; Degn, B; Wiborg, Ove.

I: Biochemistry international, Bind 28, Nr. 2, 1993, s. 353-62.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Knudsen, CR, Clark, BFC, Degn, B & Wiborg, O 1993, 'One-step purification of E. coli elongation factor Tu.', Biochemistry international, bind 28, nr. 2, s. 353-62.

APA

Knudsen, C. R., Clark, B. F. C., Degn, B., & Wiborg, O. (1993). One-step purification of E. coli elongation factor Tu. Biochemistry international, 28(2), 353-62.

CBE

Knudsen CR, Clark BFC, Degn B, Wiborg O. 1993. One-step purification of E. coli elongation factor Tu. Biochemistry international. 28(2):353-62.

MLA

Knudsen, Charlotte Rohde o.a.. "One-step purification of E. coli elongation factor Tu.". Biochemistry international. 1993, 28(2). 353-62.

Vancouver

Knudsen CR, Clark BFC, Degn B, Wiborg O. One-step purification of E. coli elongation factor Tu. Biochemistry international. 1993;28(2):353-62.

Author

Knudsen, Charlotte Rohde ; Clark, Brian F. C. ; Degn, B ; Wiborg, Ove. / One-step purification of E. coli elongation factor Tu. I: Biochemistry international. 1993 ; Bind 28, Nr. 2. s. 353-62.

Bibtex

@article{392dbe50da4511dcbc43000ea68e967b,
title = "One-step purification of E. coli elongation factor Tu.",
abstract = "The tuf A gene, encoding the E. coli elongation factor Tu, was cloned in the pGEX gene fusion system. Upon expression EF-Tu is fused to glutathione-S-transferase serving as a purification handle with affinity for glutathione immobilised on agarose. This allows purification of EF-Tu in a one-step procedure. The construct was designed in order to make possible the release of authentic EF-Tu by cleaving the fusion protein with the protease factor Xa. Udgivelsesdato: 1992-Oct",
keywords = "Amino Acid Sequence, Anti-Bacterial Agents, Cloning, Molecular, Electrophoresis, Polyacrylamide Gel, Escherichia coli, Factor Xa, Gene Expression, Glutathione Transferase, Molecular Sequence Data, Mutagenesis, Site-Directed, Peptide Elongation Factor Tu, Plasmids, Pyridones, Recombinant Fusion Proteins",
author = "Knudsen, {Charlotte Rohde} and Clark, {Brian F. C.} and B Degn and Ove Wiborg",
year = "1993",
language = "English",
volume = "28",
pages = "353--62",
journal = "Biochemistry international",
issn = "0158-5231",
publisher = "Academic Press Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - One-step purification of E. coli elongation factor Tu.

AU - Knudsen, Charlotte Rohde

AU - Clark, Brian F. C.

AU - Degn, B

AU - Wiborg, Ove

PY - 1993

Y1 - 1993

N2 - The tuf A gene, encoding the E. coli elongation factor Tu, was cloned in the pGEX gene fusion system. Upon expression EF-Tu is fused to glutathione-S-transferase serving as a purification handle with affinity for glutathione immobilised on agarose. This allows purification of EF-Tu in a one-step procedure. The construct was designed in order to make possible the release of authentic EF-Tu by cleaving the fusion protein with the protease factor Xa. Udgivelsesdato: 1992-Oct

AB - The tuf A gene, encoding the E. coli elongation factor Tu, was cloned in the pGEX gene fusion system. Upon expression EF-Tu is fused to glutathione-S-transferase serving as a purification handle with affinity for glutathione immobilised on agarose. This allows purification of EF-Tu in a one-step procedure. The construct was designed in order to make possible the release of authentic EF-Tu by cleaving the fusion protein with the protease factor Xa. Udgivelsesdato: 1992-Oct

KW - Amino Acid Sequence

KW - Anti-Bacterial Agents

KW - Cloning, Molecular

KW - Electrophoresis, Polyacrylamide Gel

KW - Escherichia coli

KW - Factor Xa

KW - Gene Expression

KW - Glutathione Transferase

KW - Molecular Sequence Data

KW - Mutagenesis, Site-Directed

KW - Peptide Elongation Factor Tu

KW - Plasmids

KW - Pyridones

KW - Recombinant Fusion Proteins

M3 - Journal article

VL - 28

SP - 353

EP - 362

JO - Biochemistry international

JF - Biochemistry international

SN - 0158-5231

IS - 2

ER -