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Charlotte Rohde Knudsen

One-step purification of E. coli elongation factor Tu.

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  • Interdisciplinary Nanoscience Center
  • Molekylærbiologisk Institut
  • Center for psykiatrisk forskning
The tuf A gene, encoding the E. coli elongation factor Tu, was cloned in the pGEX gene fusion system. Upon expression EF-Tu is fused to glutathione-S-transferase serving as a purification handle with affinity for glutathione immobilised on agarose. This allows purification of EF-Tu in a one-step procedure. The construct was designed in order to make possible the release of authentic EF-Tu by cleaving the fusion protein with the protease factor Xa.
Udgivelsesdato: 1992-Oct
OriginalsprogEngelsk
TidsskriftBiochemistry international
Vol/bind28
Nummer2
Sider (fra-til)353-62
Antal sider9
ISSN0158-5231
StatusUdgivet - 1993

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