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Charlotte Rohde Knudsen

Characterization of human mucin (MUC15) and identification of ovine and caprine orthologs

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Characterization of human mucin (MUC15) and identification of ovine and caprine orthologs. / Pallesen, Lone Tjener; Pedersen, Lise Refstrup Linnebjerg; Petersen, Torben Ellebæk; Knudsen, Charlotte Rohde; Rasmussen, Jan Trige.

I: Journal of Dairy Science, Bind 91, Nr. 12, 2008, s. 4477-83.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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@article{18e82a30e6cf11dd8fc3000ea68e967b,
title = "Characterization of human mucin (MUC15) and identification of ovine and caprine orthologs",
abstract = "The glycoprotein MUC15 (mucin 15) was initially isolated from the bovine milk fat globule membrane. The present work demonstrates the existence of immunologically similar proteins ( approximately 130 kDa) in ovine, caprine, porcine, and buffalo milk samples. Purification and N-terminal amino acid sequencing confirmed the presence of ovine and caprine MUC15 orthologs in milk fat globule membranes. Expression of MUC15 in human milk was demonstrated by immunostaining ( approximately 150 kDa) as well as by mass spectrometry. Screening of a human multiple tissue expression array showed abundant MUC15 gene expression in placenta, salivary gland, thyroid gland, trachea, esophagus, kidney, testis, and the leukemia K-562 cell line. Furthermore, moderate expression was seen in the pancreas, adult and fetal lung, fetal kidney, lymph node, adult and fetal thymus, and parietal lobe. Structural motifs for interactions (epidermal growth factor receptor and Src homology 2 domains) are identified in the intracellular region. Implication of the mucin in signal transduction and the potential physiological function of MUC15 are discussed.",
keywords = "MUC15, ortholog, milk fat globule membrane, mucin expression",
author = "Pallesen, {Lone Tjener} and Pedersen, {Lise Refstrup Linnebjerg} and Petersen, {Torben Elleb{\ae}k} and Knudsen, {Charlotte Rohde} and Rasmussen, {Jan Trige}",
note = "Paper id:: 19038922",
year = "2008",
language = "English",
volume = "91",
pages = "4477--83",
journal = "Journal of Dairy Science",
issn = "0022-0302",
publisher = "Elsevier Inc",
number = "12",

}

RIS

TY - JOUR

T1 - Characterization of human mucin (MUC15) and identification of ovine and caprine orthologs

AU - Pallesen, Lone Tjener

AU - Pedersen, Lise Refstrup Linnebjerg

AU - Petersen, Torben Ellebæk

AU - Knudsen, Charlotte Rohde

AU - Rasmussen, Jan Trige

N1 - Paper id:: 19038922

PY - 2008

Y1 - 2008

N2 - The glycoprotein MUC15 (mucin 15) was initially isolated from the bovine milk fat globule membrane. The present work demonstrates the existence of immunologically similar proteins ( approximately 130 kDa) in ovine, caprine, porcine, and buffalo milk samples. Purification and N-terminal amino acid sequencing confirmed the presence of ovine and caprine MUC15 orthologs in milk fat globule membranes. Expression of MUC15 in human milk was demonstrated by immunostaining ( approximately 150 kDa) as well as by mass spectrometry. Screening of a human multiple tissue expression array showed abundant MUC15 gene expression in placenta, salivary gland, thyroid gland, trachea, esophagus, kidney, testis, and the leukemia K-562 cell line. Furthermore, moderate expression was seen in the pancreas, adult and fetal lung, fetal kidney, lymph node, adult and fetal thymus, and parietal lobe. Structural motifs for interactions (epidermal growth factor receptor and Src homology 2 domains) are identified in the intracellular region. Implication of the mucin in signal transduction and the potential physiological function of MUC15 are discussed.

AB - The glycoprotein MUC15 (mucin 15) was initially isolated from the bovine milk fat globule membrane. The present work demonstrates the existence of immunologically similar proteins ( approximately 130 kDa) in ovine, caprine, porcine, and buffalo milk samples. Purification and N-terminal amino acid sequencing confirmed the presence of ovine and caprine MUC15 orthologs in milk fat globule membranes. Expression of MUC15 in human milk was demonstrated by immunostaining ( approximately 150 kDa) as well as by mass spectrometry. Screening of a human multiple tissue expression array showed abundant MUC15 gene expression in placenta, salivary gland, thyroid gland, trachea, esophagus, kidney, testis, and the leukemia K-562 cell line. Furthermore, moderate expression was seen in the pancreas, adult and fetal lung, fetal kidney, lymph node, adult and fetal thymus, and parietal lobe. Structural motifs for interactions (epidermal growth factor receptor and Src homology 2 domains) are identified in the intracellular region. Implication of the mucin in signal transduction and the potential physiological function of MUC15 are discussed.

KW - MUC15, ortholog, milk fat globule membrane, mucin expression

M3 - Journal article

VL - 91

SP - 4477

EP - 4483

JO - Journal of Dairy Science

JF - Journal of Dairy Science

SN - 0022-0302

IS - 12

ER -