Carl-Otto Ottosen

Phenotyping of wheat cultivars for heat tolerance using chlorophyll a fluorescence

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

  • Dew Kumari Sharma, Københavns Universitet, Danmark
  • Svend Bode Andersen, Københavns Universitet, Danmark
  • Carl-Otto Ottosen
  • Eva Rosenqvist, Afgrødevidenskab, Danmark
Abstract. In view of the global climate change, heat stress is an increasing constraint for the productivity of wheat (Triticum aestivum L.). Our aim was to identify contrasting cultivars in terms of heat tolerance by mass screening of 1274 wheat cultivars of diverse origin, based on a physiological trait, the maximum quantum efficiency of PSII (Fv/Fm).
A chlorophyll fluorescence protocol was standardised and used for repeated screening with increased selection pressure with a view to identifying a set of cultivars extreme for the trait. An initial mass screening of 1274 wheat cultivars with a milder heat stress of 38Cin 300 mmolm–2 s–1 for 2 h with preheating at 33–35Cfor 19 h in 7–14 mmolm–2 s–1 light showed a genetic determination of 8.52.7%.Aheat treatment of 40Cin 300 mmolm–2 s–1 for 72 h in the second screening with 138 selected cultivars resulted in larger differentiation of cultivars with an increased genetic component (15.43.6%), which was further increased to 27.96.8% in the third screening with 41 contrasting cultivars. This contrasting set of cultivars was then used to compare the ability of chlorophyll fluorescence parameters to detect genetic difference in heat tolerance. The
identification of a set of wheat cultivars contrasting for their inherent photochemical efficiency may aid future studies to understand the genetic and physiological nature of heat stress tolerance in order to dissect quantitative traits into simpler genetic factors.
OriginalsprogEngelsk
TidsskriftFunctional Plant Biology
Vol/bind39
Nummer11
Sider (fra-til)936-947
Antal sider12
ISSN1445-4408
DOI
StatusUdgivet - 2012

    Forskningsområder

  • genetic determination, Photosynthesis, Screening, Selection, Stress, Temperature

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