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Carl-Otto Ottosen

High-throughput sequencing reveals the change of microRNA expression caused by allopolyploidization in Cucumis

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  • X. Yu, Nanjing Agricultural University, National Key Laboratory of Crop Genetics and Germplasm Enhancement
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  • Z. Zhu, Nanjing Agricultural University, National Key Laboratory of Crop Genetics and Germplasm Enhancement
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  • T. Zhang, Shanghai Qingpu Vegetable Technology Promotion Station
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  • J Li, Nanjing Agricultural University, National Key Laboratory of Crop Genetics and Germplasm Enhancement
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  • C. Cheng, Nanjing Agricultural University, National Key Laboratory of Crop Genetics and Germplasm Enhancement
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  • Q. Lou, Nanjing Agricultural University, National Key Laboratory of Crop Genetics and Germplasm Enhancement
  • ,
  • Carl-Otto Ottosen
  • J. Chen, Nanjing Agricultural University, National Key Laboratory of Crop Genetics and Germplasm Enhancement

Allopolyploidy is long recognized as an essential driving force in plant evolution. Recent studies have demonstrated that small RNAs, including microRNAs (miRNAs), play important roles in the process of allopolyploidy. However, the question that how the distinct parent-of-origin miRNAs are maintained in allopolyploids and how these small RNAs affect gene expression and phenotype remain to be answered. Therefore, we investigated the miRNA expression profiles of a synthesized allotetraploid, Cucumis xhytivus and its parents. The different developmental stages of leaves of C. xhytivus showing contrasting leaf colour were compared as well. Following high-throughput sequencing. 546 conserved d 287 novel miRNAs were identified. The expressions of nine miRNAs obtained by real-time quantitative PCR were consistent with the sequencing results. We detected that 15 miRNAs were divergently expressed between the parent species, and 23 miRNAs were differentially expressed in C. xbytivus compared to either of its parents or both, which suggests the significant effect of allopolyploidization on miRNAs accumulation. Additionally, 26 conserved and 13 novel miRNAs differed in expression between the young and mature leaves of C. xhytivus, indicating an essential role of miRNA-mediated regulation of leaf development following allopolyploidization. This study enriches the context of allopolyploidy effect on miRNAs and lays a foundation for the elucidation of the miRNA-mediated regulatory mechanism in phenotypic variation in allopolyploids.

OriginalsprogEngelsk
TidsskriftBiologia Plantarum
Vol/bind64
Sider (fra-til)104-109
Antal sider6
ISSN0006-3134
DOI
StatusUdgivet - 2020

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