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Carl-Otto Ottosen

High Throughput Sequencing of CircRNAs in Tomato Leaves Responding to Multiple Stresses of Drought and Heat

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High Throughput Sequencing of CircRNAs in Tomato Leaves Responding to Multiple Stresses of Drought and Heat. / Zhou, Rong; Yu, Xiaqing; Ottosen, Carl-Otto; Zhao, Tongmin.

I: Horticultural Plant Journal, 30.09.2019.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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@article{a97237cf76384a62a1f529347ccd2381,
title = "High Throughput Sequencing of CircRNAs in Tomato Leaves Responding to Multiple Stresses of Drought and Heat",
abstract = "Our aim is to study the roles of a new emerging group of non-coding RNAs, circRNAs, in tomato (Solanum lycopersicum L) plants grown at the combination of drought and heat, two of the most usual stress conditions known to frequently happen in field. Tomato seedlings from cultivar ‘JinlingMeiyu’ were treated without stresses (control), at water shortage, at high temperature and subjected the multiple stresses. In total, 467 circRNAs were identified with 87.82{\%} from exon using high throughput sequencing technology. Among the circRNAs, 70 were from chr1 with the range from 23 to 49 from the other chromosomes. In detail, 156 circRNAs were shared in the four libraries, while 21, 17 and 36 circRNAs were only shown in drought, heat and multiple stresses libraries, respectively. Through a differential expression analysis, four, seven and nine circRNAs were differentially regulated in tomato at drought, heat and multiple stresses as compared with control. These circRNAs played roles on photosynthesis, starch and sucrose metabolism, RNA transport, RNA degradation, spliceosome, ribosome, etc. Our study underlined the potential role of circRNAs involved in the abiotic stress response in tomato, which might pave the way for studying biological roles of circRNAs responding to multiple stresses in plants.",
keywords = "Solanum lycopersicum, circRNAs, high throughput sequencing, high temperature, water shortage",
author = "Rong Zhou and Xiaqing Yu and Carl-Otto Ottosen and Tongmin Zhao",
year = "2019",
month = "9",
day = "30",
doi = "10.1016/j.hpj.2019.12.004",
language = "English",
journal = "Horticultural Plant Journal",
issn = "2468-0141",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - High Throughput Sequencing of CircRNAs in Tomato Leaves Responding to Multiple Stresses of Drought and Heat

AU - Zhou, Rong

AU - Yu, Xiaqing

AU - Ottosen, Carl-Otto

AU - Zhao, Tongmin

PY - 2019/9/30

Y1 - 2019/9/30

N2 - Our aim is to study the roles of a new emerging group of non-coding RNAs, circRNAs, in tomato (Solanum lycopersicum L) plants grown at the combination of drought and heat, two of the most usual stress conditions known to frequently happen in field. Tomato seedlings from cultivar ‘JinlingMeiyu’ were treated without stresses (control), at water shortage, at high temperature and subjected the multiple stresses. In total, 467 circRNAs were identified with 87.82% from exon using high throughput sequencing technology. Among the circRNAs, 70 were from chr1 with the range from 23 to 49 from the other chromosomes. In detail, 156 circRNAs were shared in the four libraries, while 21, 17 and 36 circRNAs were only shown in drought, heat and multiple stresses libraries, respectively. Through a differential expression analysis, four, seven and nine circRNAs were differentially regulated in tomato at drought, heat and multiple stresses as compared with control. These circRNAs played roles on photosynthesis, starch and sucrose metabolism, RNA transport, RNA degradation, spliceosome, ribosome, etc. Our study underlined the potential role of circRNAs involved in the abiotic stress response in tomato, which might pave the way for studying biological roles of circRNAs responding to multiple stresses in plants.

AB - Our aim is to study the roles of a new emerging group of non-coding RNAs, circRNAs, in tomato (Solanum lycopersicum L) plants grown at the combination of drought and heat, two of the most usual stress conditions known to frequently happen in field. Tomato seedlings from cultivar ‘JinlingMeiyu’ were treated without stresses (control), at water shortage, at high temperature and subjected the multiple stresses. In total, 467 circRNAs were identified with 87.82% from exon using high throughput sequencing technology. Among the circRNAs, 70 were from chr1 with the range from 23 to 49 from the other chromosomes. In detail, 156 circRNAs were shared in the four libraries, while 21, 17 and 36 circRNAs were only shown in drought, heat and multiple stresses libraries, respectively. Through a differential expression analysis, four, seven and nine circRNAs were differentially regulated in tomato at drought, heat and multiple stresses as compared with control. These circRNAs played roles on photosynthesis, starch and sucrose metabolism, RNA transport, RNA degradation, spliceosome, ribosome, etc. Our study underlined the potential role of circRNAs involved in the abiotic stress response in tomato, which might pave the way for studying biological roles of circRNAs responding to multiple stresses in plants.

KW - Solanum lycopersicum

KW - circRNAs

KW - high throughput sequencing

KW - high temperature

KW - water shortage

U2 - 10.1016/j.hpj.2019.12.004

DO - 10.1016/j.hpj.2019.12.004

M3 - Journal article

JO - Horticultural Plant Journal

JF - Horticultural Plant Journal

SN - 2468-0141

ER -