Aarhus University Seal / Aarhus Universitets segl

Carl-Otto Ottosen

High Throughput Sequencing of CircRNAs in Tomato Leaves Responding to Multiple Stresses of Drought and Heat

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

  • Rong Zhou
  • Xiaqing Yu
  • ,
  • Carl-Otto Ottosen
  • Tongmin Zhao, Jiangsu Province Academy of Agricultural Science, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Jiangsu Province Academy of Agricultural Sciences
Our aim is to study the roles of a new emerging group of non-coding RNAs, circRNAs, in tomato (Solanum lycopersicum L) plants grown at the combination of drought and heat, two of the most usual stress conditions known to frequently happen in field. Tomato seedlings from cultivar ‘JinlingMeiyu’ were treated without stresses (control), at water shortage, at high temperature and subjected the multiple stresses. In total, 467 circRNAs were identified with 87.82% from exon using high throughput sequencing technology. Among the circRNAs, 70 were from chr1 with the range from 23 to 49 from the other chromosomes. In detail, 156 circRNAs were shared in the four libraries, while 21, 17 and 36 circRNAs were only shown in drought, heat and multiple stresses libraries, respectively. Through a differential expression analysis, four, seven and nine circRNAs were differentially regulated in tomato at drought, heat and multiple stresses as compared with control. These circRNAs played roles on photosynthesis, starch and sucrose metabolism, RNA transport, RNA degradation, spliceosome, ribosome, etc. Our study underlined the potential role of circRNAs involved in the abiotic stress response in tomato, which might pave the way for studying biological roles of circRNAs responding to multiple stresses in plants.
OriginalsprogEngelsk
TidsskriftHorticultural Plant Journal
ISSN2468-0141
StatusUdgivet - 30 sep. 2019

    Forskningsområder

  • Solanum lycopersicum, circRNAs, high throughput sequencing, high temperature, water shortage

Se relationer på Aarhus Universitet Citationsformater

ID: 169169712