Institut for Biomedicin

Birgitte Mønster Christensen

Axial heterogeneity in basolateral AQP2 localization in rat kidney: effect of vasopressin

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Axial heterogeneity in basolateral AQP2 localization in rat kidney: effect of vasopressin. / Christensen, Birgitte Mønster; Wang, Weidong; Frøkiaer, Jørgen; Nielsen, Søren.

I: American Journal of Physiology: Renal Physiology, Bind 284, Nr. 4, 2002, s. F701-17.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Christensen, BM, Wang, W, Frøkiaer, J & Nielsen, S 2002, 'Axial heterogeneity in basolateral AQP2 localization in rat kidney: effect of vasopressin', American Journal of Physiology: Renal Physiology, bind 284, nr. 4, s. F701-17. https://doi.org/10.1152/ajprenal.00234.2002

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Author

Christensen, Birgitte Mønster ; Wang, Weidong ; Frøkiaer, Jørgen ; Nielsen, Søren. / Axial heterogeneity in basolateral AQP2 localization in rat kidney: effect of vasopressin. I: American Journal of Physiology: Renal Physiology. 2002 ; Bind 284, Nr. 4. s. F701-17.

Bibtex

@article{5cbab5a0cc4611dd9710000ea68e967b,
title = "Axial heterogeneity in basolateral AQP2 localization in rat kidney: effect of vasopressin",
abstract = "The purpose of the present study was to examine whether there is axial heterogeneity in the basolateral plasma membrane (BLM) localization of AQP2 and whether altered vasopressin action or medullary tonicity affects the BLM localization of AQP2. Immunocytochemistry and immunoelectron microscopy revealed AQP2 labeling of the BLM in connecting tubule (CNT) cells and inner medullary collecting duct (IMCD) principal cells in normal rats and vasopressin-deficient Brattleboro rats. In contrast there was little basolateral AQP2 labeling in cortical (CCD) and outer medullary collecting duct principal cells. Short-term desamino-Cys(1), (D)-Arg(8) vasopressin (dDAVP) treatment (2 h) of Brattleboro rats caused no increase in AQP2 labeling of the BLM. In contrast, long-term dDAVP treatment (6 days) of Brattleboro rats caused an increased BLM labeling in CNT, CCD, and IMCD. Treatment of normal rats with V(2)-receptor antagonist for 60 min caused retrieval of AQP2 from the apical plasma membrane. Moreover, AQP2 labeling of the BLM was unchanged in CNT and IMCD but increased in CCD. In conclusion, there is an axial heterogeneity in the subcellular localization of AQP2 with prominent AQP2 labeling of the BLM in CNT and IMCD. There was no increase in AQP2 labeling of the BLM in response to short-term dDAVP. Moreover, acute V(2)-receptor antagonist treatment did not cause retrieval of AQP2 from the BLM. In contrast, long-term dDAVP treatment caused a major increase in AQP2 expression in the BLM in CCD.",
keywords = "Animals, Aquaporin 2, Aquaporin 6, Aquaporins, Cell Membrane, Deamino Arginine Vasopressin, Immunohistochemistry, Kidney Cortex, Kidney Tubules, Male, Rats, Rats, Brattleboro, Rats, Wistar, Receptors, Vasopressin, Renal Agents, Vasopressins",
author = "Christensen, {Birgitte M{\o}nster} and Weidong Wang and J{\o}rgen Fr{\o}kiaer and S{\o}ren Nielsen",
year = "2002",
doi = "10.1152/ajprenal.00234.2002",
language = "English",
volume = "284",
pages = "F701--17",
journal = "American Journal of Physiology: Renal Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "4",

}

RIS

TY - JOUR

T1 - Axial heterogeneity in basolateral AQP2 localization in rat kidney: effect of vasopressin

AU - Christensen, Birgitte Mønster

AU - Wang, Weidong

AU - Frøkiaer, Jørgen

AU - Nielsen, Søren

PY - 2002

Y1 - 2002

N2 - The purpose of the present study was to examine whether there is axial heterogeneity in the basolateral plasma membrane (BLM) localization of AQP2 and whether altered vasopressin action or medullary tonicity affects the BLM localization of AQP2. Immunocytochemistry and immunoelectron microscopy revealed AQP2 labeling of the BLM in connecting tubule (CNT) cells and inner medullary collecting duct (IMCD) principal cells in normal rats and vasopressin-deficient Brattleboro rats. In contrast there was little basolateral AQP2 labeling in cortical (CCD) and outer medullary collecting duct principal cells. Short-term desamino-Cys(1), (D)-Arg(8) vasopressin (dDAVP) treatment (2 h) of Brattleboro rats caused no increase in AQP2 labeling of the BLM. In contrast, long-term dDAVP treatment (6 days) of Brattleboro rats caused an increased BLM labeling in CNT, CCD, and IMCD. Treatment of normal rats with V(2)-receptor antagonist for 60 min caused retrieval of AQP2 from the apical plasma membrane. Moreover, AQP2 labeling of the BLM was unchanged in CNT and IMCD but increased in CCD. In conclusion, there is an axial heterogeneity in the subcellular localization of AQP2 with prominent AQP2 labeling of the BLM in CNT and IMCD. There was no increase in AQP2 labeling of the BLM in response to short-term dDAVP. Moreover, acute V(2)-receptor antagonist treatment did not cause retrieval of AQP2 from the BLM. In contrast, long-term dDAVP treatment caused a major increase in AQP2 expression in the BLM in CCD.

AB - The purpose of the present study was to examine whether there is axial heterogeneity in the basolateral plasma membrane (BLM) localization of AQP2 and whether altered vasopressin action or medullary tonicity affects the BLM localization of AQP2. Immunocytochemistry and immunoelectron microscopy revealed AQP2 labeling of the BLM in connecting tubule (CNT) cells and inner medullary collecting duct (IMCD) principal cells in normal rats and vasopressin-deficient Brattleboro rats. In contrast there was little basolateral AQP2 labeling in cortical (CCD) and outer medullary collecting duct principal cells. Short-term desamino-Cys(1), (D)-Arg(8) vasopressin (dDAVP) treatment (2 h) of Brattleboro rats caused no increase in AQP2 labeling of the BLM. In contrast, long-term dDAVP treatment (6 days) of Brattleboro rats caused an increased BLM labeling in CNT, CCD, and IMCD. Treatment of normal rats with V(2)-receptor antagonist for 60 min caused retrieval of AQP2 from the apical plasma membrane. Moreover, AQP2 labeling of the BLM was unchanged in CNT and IMCD but increased in CCD. In conclusion, there is an axial heterogeneity in the subcellular localization of AQP2 with prominent AQP2 labeling of the BLM in CNT and IMCD. There was no increase in AQP2 labeling of the BLM in response to short-term dDAVP. Moreover, acute V(2)-receptor antagonist treatment did not cause retrieval of AQP2 from the BLM. In contrast, long-term dDAVP treatment caused a major increase in AQP2 expression in the BLM in CCD.

KW - Animals

KW - Aquaporin 2

KW - Aquaporin 6

KW - Aquaporins

KW - Cell Membrane

KW - Deamino Arginine Vasopressin

KW - Immunohistochemistry

KW - Kidney Cortex

KW - Kidney Tubules

KW - Male

KW - Rats

KW - Rats, Brattleboro

KW - Rats, Wistar

KW - Receptors, Vasopressin

KW - Renal Agents

KW - Vasopressins

U2 - 10.1152/ajprenal.00234.2002

DO - 10.1152/ajprenal.00234.2002

M3 - Journal article

C2 - 12453871

VL - 284

SP - F701-17

JO - American Journal of Physiology: Renal Physiology

JF - American Journal of Physiology: Renal Physiology

SN - 1931-857X

IS - 4

ER -