Institut for Biomedicin

Annemarie Brüel

The effect of PTH(1-34) on fracture healing during different loading conditions

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

The effect of PTH(1-34) on fracture healing during different loading conditions. / Ellegaard, Maria; Kringelbach, Tina; Syberg, Susanne; Petersen, Solveig; Jensen, Jens-Erik Beck; Brüel, Annemarie; Jørgensen, Niklas Rye; Schwarz, Peter.

I: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 13.04.2013.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Ellegaard, M, Kringelbach, T, Syberg, S, Petersen, S, Jensen, J-EB, Brüel, A, Jørgensen, NR & Schwarz, P 2013, 'The effect of PTH(1-34) on fracture healing during different loading conditions', Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. https://doi.org/10.1002/jbmr.1957

APA

Ellegaard, M., Kringelbach, T., Syberg, S., Petersen, S., Jensen, J-E. B., Brüel, A., Jørgensen, N. R., & Schwarz, P. (2013). The effect of PTH(1-34) on fracture healing during different loading conditions. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. https://doi.org/10.1002/jbmr.1957

CBE

Ellegaard M, Kringelbach T, Syberg S, Petersen S, Jensen J-EB, Brüel A, Jørgensen NR, Schwarz P. 2013. The effect of PTH(1-34) on fracture healing during different loading conditions. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. https://doi.org/10.1002/jbmr.1957

MLA

Ellegaard, Maria o.a.. "The effect of PTH(1-34) on fracture healing during different loading conditions". Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 2013. https://doi.org/10.1002/jbmr.1957

Vancouver

Ellegaard M, Kringelbach T, Syberg S, Petersen S, Jensen J-EB, Brüel A o.a. The effect of PTH(1-34) on fracture healing during different loading conditions. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 2013 apr 13. https://doi.org/10.1002/jbmr.1957

Author

Ellegaard, Maria ; Kringelbach, Tina ; Syberg, Susanne ; Petersen, Solveig ; Jensen, Jens-Erik Beck ; Brüel, Annemarie ; Jørgensen, Niklas Rye ; Schwarz, Peter. / The effect of PTH(1-34) on fracture healing during different loading conditions. I: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 2013.

Bibtex

@article{5d34a27459714e6eac5846115150f949,
title = "The effect of PTH(1-34) on fracture healing during different loading conditions",
abstract = "Parathyroid hormone (PTH) and PTH(1-34) have been shown to promote bone healing in several animal studies. It is known that the mechanical environment is important in fracture healing. Furthermore, PTH and mechanical loading has been suggested to have synergistic effects on intact bone. The aim of the present study was to investigate whether the effect of PTH(1-34) on fracture healing in rats was influenced by reduced mechanical loading. For this purpose we used female, 25-week-old ovariectomized rats. Animals were subjected to closed midshaft fracture of the right tibia ten weeks after ovariectomy. Five days before fracture, half of the animals received Botulinum Toxin A-injections in the muscles of the fractured leg to induce muscle paralysis (unloaded group), while the other half received saline injections (control group). For the following eight weeks, half of the animals in each group received injections of hPTH(1-34) (20 µg/kg/day) and the other half received vehicle treatment. Fracture healing was assessed by radiology, DXA, histology and bone strength analysis. We found that unloading reduced callus area significantly, while no effects of PTH(1-34) on callus area were seen in neither normally nor unloaded animals. PTH(1-34) increased callus bone mineral density (BMD) and bone mineral content (BMC) significantly, while unloading decreased callus BMD and BMC significantly. PTH(1-34)-treatment increased bone volume of the callus in both unloaded and control animals. PTH(1-34)-treatment increased ultimate force of the fracture by 63% in both control and unloaded animals and no interaction of the two interventions could be detected. PTH(1-34) was able to stimulate bone formation in normally loaded as well as unloaded intact bone. In conclusion, the study confirms the stimulatory effect of PTH(1-34) on fracture healing and our data suggest that PTH(1-34) is able to promote fracture healing, as well as intact bone formation during conditions of reduced mechanical loading.",
author = "Maria Ellegaard and Tina Kringelbach and Susanne Syberg and Solveig Petersen and Jensen, {Jens-Erik Beck} and Annemarie Br{\"u}el and J{\o}rgensen, {Niklas Rye} and Peter Schwarz",
note = "Copyright {\textcopyright} 2013 American Society for Bone and Mineral Research.",
year = "2013",
month = apr,
day = "13",
doi = "10.1002/jbmr.1957",
language = "English",
journal = "Journal of Bone and Mineral Research",
issn = "0884-0431",
publisher = "Wiley-Blackwell Publishing, Inc.",

}

RIS

TY - JOUR

T1 - The effect of PTH(1-34) on fracture healing during different loading conditions

AU - Ellegaard, Maria

AU - Kringelbach, Tina

AU - Syberg, Susanne

AU - Petersen, Solveig

AU - Jensen, Jens-Erik Beck

AU - Brüel, Annemarie

AU - Jørgensen, Niklas Rye

AU - Schwarz, Peter

N1 - Copyright © 2013 American Society for Bone and Mineral Research.

PY - 2013/4/13

Y1 - 2013/4/13

N2 - Parathyroid hormone (PTH) and PTH(1-34) have been shown to promote bone healing in several animal studies. It is known that the mechanical environment is important in fracture healing. Furthermore, PTH and mechanical loading has been suggested to have synergistic effects on intact bone. The aim of the present study was to investigate whether the effect of PTH(1-34) on fracture healing in rats was influenced by reduced mechanical loading. For this purpose we used female, 25-week-old ovariectomized rats. Animals were subjected to closed midshaft fracture of the right tibia ten weeks after ovariectomy. Five days before fracture, half of the animals received Botulinum Toxin A-injections in the muscles of the fractured leg to induce muscle paralysis (unloaded group), while the other half received saline injections (control group). For the following eight weeks, half of the animals in each group received injections of hPTH(1-34) (20 µg/kg/day) and the other half received vehicle treatment. Fracture healing was assessed by radiology, DXA, histology and bone strength analysis. We found that unloading reduced callus area significantly, while no effects of PTH(1-34) on callus area were seen in neither normally nor unloaded animals. PTH(1-34) increased callus bone mineral density (BMD) and bone mineral content (BMC) significantly, while unloading decreased callus BMD and BMC significantly. PTH(1-34)-treatment increased bone volume of the callus in both unloaded and control animals. PTH(1-34)-treatment increased ultimate force of the fracture by 63% in both control and unloaded animals and no interaction of the two interventions could be detected. PTH(1-34) was able to stimulate bone formation in normally loaded as well as unloaded intact bone. In conclusion, the study confirms the stimulatory effect of PTH(1-34) on fracture healing and our data suggest that PTH(1-34) is able to promote fracture healing, as well as intact bone formation during conditions of reduced mechanical loading.

AB - Parathyroid hormone (PTH) and PTH(1-34) have been shown to promote bone healing in several animal studies. It is known that the mechanical environment is important in fracture healing. Furthermore, PTH and mechanical loading has been suggested to have synergistic effects on intact bone. The aim of the present study was to investigate whether the effect of PTH(1-34) on fracture healing in rats was influenced by reduced mechanical loading. For this purpose we used female, 25-week-old ovariectomized rats. Animals were subjected to closed midshaft fracture of the right tibia ten weeks after ovariectomy. Five days before fracture, half of the animals received Botulinum Toxin A-injections in the muscles of the fractured leg to induce muscle paralysis (unloaded group), while the other half received saline injections (control group). For the following eight weeks, half of the animals in each group received injections of hPTH(1-34) (20 µg/kg/day) and the other half received vehicle treatment. Fracture healing was assessed by radiology, DXA, histology and bone strength analysis. We found that unloading reduced callus area significantly, while no effects of PTH(1-34) on callus area were seen in neither normally nor unloaded animals. PTH(1-34) increased callus bone mineral density (BMD) and bone mineral content (BMC) significantly, while unloading decreased callus BMD and BMC significantly. PTH(1-34)-treatment increased bone volume of the callus in both unloaded and control animals. PTH(1-34)-treatment increased ultimate force of the fracture by 63% in both control and unloaded animals and no interaction of the two interventions could be detected. PTH(1-34) was able to stimulate bone formation in normally loaded as well as unloaded intact bone. In conclusion, the study confirms the stimulatory effect of PTH(1-34) on fracture healing and our data suggest that PTH(1-34) is able to promote fracture healing, as well as intact bone formation during conditions of reduced mechanical loading.

U2 - 10.1002/jbmr.1957

DO - 10.1002/jbmr.1957

M3 - Journal article

C2 - 23585311

JO - Journal of Bone and Mineral Research

JF - Journal of Bone and Mineral Research

SN - 0884-0431

ER -